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Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) (CBMAB-C5754-CQ)

This product is a mouse antibody that recognizes AS3MT. The antibody CBXC-1792 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all AS3MT antibodies
Published Data
Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) CBMAB-C5754-CQ in IHC
AS3MT expression by immunohistochemistry in control and iAs-treated livers. ...View More
Qiu, T., Wu, C., Yao, X., Han, Q., Wang, N., Yuan, W., ... & Sun, X. (2022). AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance. Cell Biology and Toxicology, 1-17.
Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) CBMAB-C5754-CQ in IP
AS3MT and NLRP3 binding domains were explored using full length and truncated AS3MT and NLRP3 expression plasmids and Co-IP assays, followed by western blotting. ...View More
Qiu, T., Wu, C., Yao, X., Han, Q., Wang, N., Yuan, W., ... & Sun, X. (2022). AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance. Cell Biology and Toxicology, 1-17.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXC-1792
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of cyt19 of mouse origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Arsenite Methyltransferase
Introduction
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
57412
UniProt ID
Q9HBK9
Alternative Names
Arsenite Methyltransferase; S-Adenosyl-L-Methionine:Arsenic(III) Methyltransferase; Arsenic (+3 Oxidation State) Methyltransferase; Methylarsonite Methyltransferase; CYT19; S-Adenosylmethionine:Arsenic (III) Methyltransferase; Methyltransferase Cyt19; EC 2.1.1.137;
Function
Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals (PubMed:16407288, PubMed:25997655).
It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2 (PubMed:16407288, PubMed:25997655).
Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH (PubMed:16407288, PubMed:25997655).
Biological Process
Arsonoacetate metabolic process Source: UniProtKB
Methylation Source: UniProtKB
Toxin metabolic process Source: UniProtKB
Cellular Location
Cytosol

Sun, M., Tan, J., Wang, M., Wen, W., & He, Y. (2021). Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. Environmental Toxicology, 36(2), 204-212.

Roy, N. K., Murphy, A., & Costa, M. (2020). Arsenic methyltransferase and methylation of inorganic arsenic. Biomolecules, 10(9), 1351.

Torbøl Pedersen, J., De Loma, J., Levi, M., Palmgren, M., & Broberg, K. (2020). Predicted AS3MT Proteins Methylate Arsenic and Support Two Major Phylogenetic AS3MT Groups. Chemical research in toxicology, 33(12), 3041-3047.

Liu, W. S., Wang, X. Y., Lu, J., Zhang, Y. M., Ye, X. M., Li, J. M., ... & Hai, X. (2020). Polymorphisms in arsenic (+ 3 oxidation state) methyltransferase (AS3MT) predict the occurrence of hyperleukocytosis and arsenic metabolism in APL patients treated with As 2 O 3. Archives of toxicology, 94(4), 1203-1213.

Chernoff, M., Tong, L., Demanelis, K., Vander Griend, D., Ahsan, H., & Pierce, B. L. (2020). Genetic determinants of reduced arsenic metabolism efficiency in the 10q24. 32 region are associated with reduced AS3MT expression in multiple human tissue types. Toxicological Sciences, 176(2), 382-395.

Lin, Y. C., Chen, W. J., Huang, C. Y., Shiue, H. S., Su, C. T., Ao, P. L., ... & Hsueh, Y. M. (2018). Polymorphisms of arsenic (+ 3 oxidation state) methyltransferase and arsenic methylation capacity affect the risk of bladder cancer. Toxicological Sciences, 164(1), 328-338.

García-Alvarado, F. J., & Neri-Meléndez, H. (2018). Polymorphisms of the arsenite methyltransferase (AS3MT) gene and urinary efficiency of arsenic metabolism in a population in Northern Mexico. Revista peruana de medicina experimental y salud publica, 35(1), 72-76.

De Loma, J., Skröder, H., Raqib, R., Vahter, M., & Broberg, K. (2018). Arsenite methyltransferase (AS3MT) polymorphisms and arsenic methylation in children in rural Bangladesh. Toxicology and applied pharmacology, 357, 80-87.

De la Rosa, R., Steinmaus, C., Akers, N. K., Conde, L., Ferreccio, C., Kalman, D., ... & Smith, M. T. (2017). Associations between arsenic (+ 3 oxidation state) methyltransferase (AS3MT) and N‐6 adenine‐specific DNA methyltransferase 1 (N6AMT1) polymorphisms, arsenic metabolism, and cancer risk in a chilean population. Environmental and molecular mutagenesis, 58(6), 411-422.

Palmgren, M., Engström, K., Hallström, B. M., Wahlberg, K., Søndergaard, D. A., Säll, T., ... & Broberg, K. (2017). AS3MT-mediated tolerance to arsenic evolved by multiple independent horizontal gene transfers from bacteria to eukaryotes. PloS one, 12(4), e0175422.

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For research use only. Not intended for any clinical use.

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