Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) (CBMAB-C5754-CQ)

Name: Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792)
SKU: CBMAB-C5754-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) CBMAB-C5754-CQ in IHC

AS3MT expression by immunohistochemistry in control and iAs-treated livers. ...View More

Qiu, T., Wu, C., Yao, X., Han, Q., Wang, N., Yuan, W., ... & Sun, X. (2022). AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance. Cell Biology and Toxicology, 1-17.

Mouse Anti-AS3MT Recombinant Antibody (CBXC-1792) CBMAB-C5754-CQ in IP

AS3MT and NLRP3 binding domains were explored using full length and truncated AS3MT and NLRP3 expression plasmids and Co-IP assays, followed by western blotting. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1792

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-300 mapping at the N-terminus of cyt19 of mouse origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Arsenite Methyltransferase

Introduction

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

57412

UniProt ID

Q9HBK9

Alternative Names

Arsenite Methyltransferase; S-Adenosyl-L-Methionine:Arsenic(III) Methyltransferase; Arsenic (+3 Oxidation State) Methyltransferase; Methylarsonite Methyltransferase; CYT19; S-Adenosylmethionine:Arsenic (III) Methyltransferase; Methyltransferase Cyt19; EC 2.1.1.137;

Function

Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals (PubMed:16407288, PubMed:25997655).
It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2 (PubMed:16407288, PubMed:25997655).
Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH (PubMed:16407288, PubMed:25997655).

Biological Process

Arsonoacetate metabolic process Source: UniProtKB
Methylation Source: UniProtKB
Toxin metabolic process Source: UniProtKB

Cellular Location

Cytosol

Sun, M., Tan, J., Wang, M., Wen, W., & He, Y. (2021). Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. Environmental Toxicology, 36(2), 204-212.

Roy, N. K., Murphy, A., & Costa, M. (2020). Arsenic methyltransferase and methylation of inorganic arsenic. Biomolecules, 10(9), 1351.

Torbøl Pedersen, J., De Loma, J., Levi, M., Palmgren, M., & Broberg, K. (2020). Predicted AS3MT Proteins Methylate Arsenic and Support Two Major Phylogenetic AS3MT Groups. Chemical research in toxicology, 33(12), 3041-3047.

Liu, W. S., Wang, X. Y., Lu, J., Zhang, Y. M., Ye, X. M., Li, J. M., ... & Hai, X. (2020). Polymorphisms in arsenic (+ 3 oxidation state) methyltransferase (AS3MT) predict the occurrence of hyperleukocytosis and arsenic metabolism in APL patients treated with As 2 O 3. Archives of toxicology, 94(4), 1203-1213.

Chernoff, M., Tong, L., Demanelis, K., Vander Griend, D., Ahsan, H., & Pierce, B. L. (2020). Genetic determinants of reduced arsenic metabolism efficiency in the 10q24. 32 region are associated with reduced AS3MT expression in multiple human tissue types. Toxicological Sciences, 176(2), 382-395.

Lin, Y. C., Chen, W. J., Huang, C. Y., Shiue, H. S., Su, C. T., Ao, P. L., ... & Hsueh, Y. M. (2018). Polymorphisms of arsenic (+ 3 oxidation state) methyltransferase and arsenic methylation capacity affect the risk of bladder cancer. Toxicological Sciences, 164(1), 328-338.

García-Alvarado, F. J., & Neri-Meléndez, H. (2018). Polymorphisms of the arsenite methyltransferase (AS3MT) gene and urinary efficiency of arsenic metabolism in a population in Northern Mexico. Revista peruana de medicina experimental y salud publica, 35(1), 72-76.

De Loma, J., Skröder, H., Raqib, R., Vahter, M., & Broberg, K. (2018). Arsenite methyltransferase (AS3MT) polymorphisms and arsenic methylation in children in rural Bangladesh. Toxicology and applied pharmacology, 357, 80-87.

De la Rosa, R., Steinmaus, C., Akers, N. K., Conde, L., Ferreccio, C., Kalman, D., ... & Smith, M. T. (2017). Associations between arsenic (+ 3 oxidation state) methyltransferase (AS3MT) and N‐6 adenine‐specific DNA methyltransferase 1 (N6AMT1) polymorphisms, arsenic metabolism, and cancer risk in a chilean population. Environmental and molecular mutagenesis, 58(6), 411-422.

Palmgren, M., Engström, K., Hallström, B. M., Wahlberg, K., Søndergaard, D. A., Säll, T., ... & Broberg, K. (2017). AS3MT-mediated tolerance to arsenic evolved by multiple independent horizontal gene transfers from bacteria to eukaryotes. PloS one, 12(4), e0175422.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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