Mouse As3mt ELISA Kit (V2LY-0626-LY1634)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.56 ng/mL
Detection Range
1-400 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
41.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Arsenite Methyltransferase
Function
Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals (PubMed:16407288, PubMed:25997655).
It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2 (PubMed:16407288, PubMed:25997655).
Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH (PubMed:16407288, PubMed:25997655).
Biological Process
Arsonoacetate metabolic process Source: UniProtKB
Methylation Source: UniProtKB
Toxin metabolic process Source: UniProtKB
Cellular Location
Cytosol

Sun, M., Tan, J., Wang, M., Wen, W., & He, Y. (2021). Inorganic arsenic‐mediated upregulation of AS3MT promotes proliferation of nonsmall cell lung cancer cells by regulating cell cycle genes. Environmental Toxicology, 36(2), 204-212.

Roy, N. K., Murphy, A., & Costa, M. (2020). Arsenic methyltransferase and methylation of inorganic arsenic. Biomolecules, 10(9), 1351.

Torbøl Pedersen, J., De Loma, J., Levi, M., Palmgren, M., & Broberg, K. (2020). Predicted AS3MT Proteins Methylate Arsenic and Support Two Major Phylogenetic AS3MT Groups. Chemical research in toxicology, 33(12), 3041-3047.

Liu, W. S., Wang, X. Y., Lu, J., Zhang, Y. M., Ye, X. M., Li, J. M., ... & Hai, X. (2020). Polymorphisms in arsenic (+ 3 oxidation state) methyltransferase (AS3MT) predict the occurrence of hyperleukocytosis and arsenic metabolism in APL patients treated with As 2 O 3. Archives of toxicology, 94(4), 1203-1213.

Chernoff, M., Tong, L., Demanelis, K., Vander Griend, D., Ahsan, H., & Pierce, B. L. (2020). Genetic determinants of reduced arsenic metabolism efficiency in the 10q24. 32 region are associated with reduced AS3MT expression in multiple human tissue types. Toxicological Sciences, 176(2), 382-395.

Lin, Y. C., Chen, W. J., Huang, C. Y., Shiue, H. S., Su, C. T., Ao, P. L., ... & Hsueh, Y. M. (2018). Polymorphisms of arsenic (+ 3 oxidation state) methyltransferase and arsenic methylation capacity affect the risk of bladder cancer. Toxicological Sciences, 164(1), 328-338.

García-Alvarado, F. J., & Neri-Meléndez, H. (2018). Polymorphisms of the arsenite methyltransferase (AS3MT) gene and urinary efficiency of arsenic metabolism in a population in Northern Mexico. Revista peruana de medicina experimental y salud publica, 35(1), 72-76.

De Loma, J., Skröder, H., Raqib, R., Vahter, M., & Broberg, K. (2018). Arsenite methyltransferase (AS3MT) polymorphisms and arsenic methylation in children in rural Bangladesh. Toxicology and applied pharmacology, 357, 80-87.

De la Rosa, R., Steinmaus, C., Akers, N. K., Conde, L., Ferreccio, C., Kalman, D., ... & Smith, M. T. (2017). Associations between arsenic (+ 3 oxidation state) methyltransferase (AS3MT) and N‐6 adenine‐specific DNA methyltransferase 1 (N6AMT1) polymorphisms, arsenic metabolism, and cancer risk in a chilean population. Environmental and molecular mutagenesis, 58(6), 411-422.

Palmgren, M., Engström, K., Hallström, B. M., Wahlberg, K., Søndergaard, D. A., Säll, T., ... & Broberg, K. (2017). AS3MT-mediated tolerance to arsenic evolved by multiple independent horizontal gene transfers from bacteria to eukaryotes. PloS one, 12(4), e0175422.

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For research use only. Not intended for any clinical use.

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