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Rabbit Anti-ASAH2 Recombinant Antibody (2) (CBMAB-A3705-YC)

Provided herein is a Rabbit monoclonal antibody against Mouse N-Acylsphingosine Amidohydrolase 2. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ASAH2 antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse
Clone
2
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
Recombinant mouse ASAH2 protein
Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-Acylsphingosine Amidohydrolase 2
Introduction
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosp
Entrez Gene ID
56624
UniProt ID
Q9NR71
Alternative Names
N-Acylsphingosine Amidohydrolase 2; Non-Lysosomal Ceramidase; N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2; Acylsphingosine Deacylase 2; Neutral Ceramidase; EC 3.5.1.23; N-CDase; BCDase;
Function
Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10781606, PubMed:16229686, PubMed:26190575).
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:15946935, PubMed:19345744, PubMed:24798654).
Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:11278489, PubMed:17475390).
Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16061940).
Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity).
This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).
Biological Process
Apoptotic process Source: UniProtKB-KW
Cellular response to cytokine stimulus Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Ceramide catabolic process Source: UniProtKB
Ceramide metabolic process Source: UniProtKB
Lipid digestion Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: GO_Central
Negative regulation of apoptotic signaling pathway Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Sphingosine biosynthetic process Source: UniProtKB
Sphingosine metabolic process Source: UniProtKB
Cellular Location
Neutral ceramidase: Cell membrane; Extracellular exosome; Golgi apparatus membrane; Mitochondrion; Membrane raft; Caveola. Enriched in exosomes upon stimulation by cytokine (PubMed:24798654). Enriched in caveolae and lipid rafts (By similarity). The localization to the mitochondrion could not be confirmed (PubMed:15845354).
Neutral ceramidase soluble form: Secreted
Topology
Cytoplasmic: 1-12 aa
Helical: 13-33 aa
Lumenal: 34-780 aa
PTM
Proteolytic cleavage of the N-terminus removes the signal-anchor and produces a soluble form of the protein.
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxide.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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