Rabbit Anti-ASAH2 Recombinant Antibody (2) (CBMAB-A3705-YC)

Name: Rabbit Anti-ASAH2 Recombinant Antibody (2)
SKU: CBMAB-A3705-YC
Rating: 5 (1 reviews)

See all ASAH2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

Application

ELISA, WB

Immunogen

Recombinant mouse ASAH2 protein

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

N-Acylsphingosine Amidohydrolase 2

Introduction

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosp

Entrez Gene ID

56624

UniProt ID

Q9NR71

Alternative Names

N-Acylsphingosine Amidohydrolase 2; Non-Lysosomal Ceramidase; N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2; Acylsphingosine Deacylase 2; Neutral Ceramidase; EC 3.5.1.23; N-CDase; BCDase;

Function

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10781606, PubMed:16229686, PubMed:26190575).
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:15946935, PubMed:19345744, PubMed:24798654).
Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:11278489, PubMed:17475390).
Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16061940).
Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity).
This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).

Biological Process

Apoptotic process Source: UniProtKB-KW
Cellular response to cytokine stimulus Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Ceramide catabolic process Source: UniProtKB
Ceramide metabolic process Source: UniProtKB
Lipid digestion Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: GO_Central
Negative regulation of apoptotic signaling pathway Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Sphingosine biosynthetic process Source: UniProtKB
Sphingosine metabolic process Source: UniProtKB

Cellular Location

Neutral ceramidase: Cell membrane; Extracellular exosome; Golgi apparatus membrane; Mitochondrion; Membrane raft; Caveola. Enriched in exosomes upon stimulation by cytokine (PubMed:24798654). Enriched in caveolae and lipid rafts (By similarity). The localization to the mitochondrion could not be confirmed (PubMed:15845354).
Neutral ceramidase soluble form: Secreted

Topology

Cytoplasmic: 1-12 aa
Helical: 13-33 aa
Lumenal: 34-780 aa

PTM

Proteolytic cleavage of the N-terminus removes the signal-anchor and produces a soluble form of the protein.
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxide.

Zhu, H., Klement, J. D., Lu, C., Redd, P. S., Yang, D., Smith, A. D., ... & Liu, K. (2021). Asah2 Represses the p53–Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis. The Journal of Immunology, 206(6), 1395-1404.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-ASAH2 Recombinant Antibody (2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry