Mouse Recombinant ASAH2 protein, His Tag (V2LY-0526-LY7826)

Name: Mouse Recombinant ASAH2 protein, His Tag
SKU: V2LY-0526-LY7826
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant ASAH2 protein, His Tag consist of Amino Acid: 34-756 and predicts a molecular mass of 82 kDa.

Molecule Mass

82 kDa

Sequence

Amino Acid: 34-756

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

N-Acylsphingosine Amidohydrolase 2

Function

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10781606, PubMed:16229686, PubMed:26190575).
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:15946935, PubMed:19345744, PubMed:24798654).
Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:11278489, PubMed:17475390).
Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16061940).
Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity).
This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).

Biological Process

Apoptotic process Source: UniProtKB-KW
Cellular response to cytokine stimulus Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Ceramide catabolic process Source: UniProtKB
Ceramide metabolic process Source: UniProtKB
Lipid digestion Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: GO_Central
Negative regulation of apoptotic signaling pathway Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Sphingosine biosynthetic process Source: UniProtKB
Sphingosine metabolic process Source: UniProtKB

Cellular Location

Neutral ceramidase: Cell membrane; Extracellular exosome; Golgi apparatus membrane; Mitochondrion; Membrane raft; Caveola. Enriched in exosomes upon stimulation by cytokine (PubMed:24798654). Enriched in caveolae and lipid rafts (By similarity). The localization to the mitochondrion could not be confirmed (PubMed:15845354).
Neutral ceramidase soluble form: Secreted

Topology

Cytoplasmic: 1-12 aa
Helical: 13-33 aa
Lumenal: 34-780 aa

PTM

Proteolytic cleavage of the N-terminus removes the signal-anchor and produces a soluble form of the protein.
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxide.

Zhu, H., Klement, J. D., Lu, C., Redd, P. S., Yang, D., Smith, A. D., ... & Liu, K. (2021). Asah2 Represses the p53–Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis. The Journal of Immunology, 206(6), 1395-1404.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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