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Mouse Anti-ATF3 Recombinant Antibody (6B8) (CBMAB-A3867-YC)

Provided herein is a Mouse monoclonal antibody against Human Activating Transcription Factor 3. The antibody can be used for immunoassay techniques, such as ELISA, RNAi, WB.
See all ATF3 antibodies
Published Data
Mouse Anti-ATF3 Recombinant Antibody (6B8) (CBMAB-A3867-YC) in WB
Expression levels of the selected transcription factor proteins in rabbit limbus (Lm) and cornea (Co). Cell lysates prepared from rabbit limbal and corneal epithelia were fractional on 7.5% SDS-PAGE (10 mg/lane). After transfer, the membranes were blotted with antibodies to CEBPB, CEBPD, STAT3, NANOG, POU5F1, EGR1, JUN, ATF3, ARID5B, or PAX6. The expression levels of p63 in limbus and cornea was also evaluated to show its limbal specificity. GAPDH was blotted and served as a loading control. ...View More
Hsueh, Y. J., Kuo, P. C., & Chen, J. K. (2013). Transcriptional regulators of the ΔNp63: their role in limbal epithelial cell proliferation. Journal of cellular physiology, 228(3), 536-546.
Mouse Anti-ATF3 Recombinant Antibody (6B8) (CBMAB-A3867-YC) in IHC
ATF3 localization. End, Endothelial cells; Ep, epithelial cells; I, inflammatory cells; SM, smooth muscle cells. ...View More
Roussel, L., Robins, S., Schachter, A., Bérubé, J., Hamid, Q., & Rousseau, S. (2011). Steroids and extracellular signal-regulated kinase 1/2 activity suppress activating transcription factor 3 expression in patients with severe asthma. Journal of Allergy and Clinical Immunology, 127(6), 1632-1634.
Mouse Anti-ATF3 Recombinant Antibody (6B8) (CBMAB-A3867-YC) in WB
Western blot analysis of the effect of shRNAmediated repression of KLF6 on CG-12-induced expression of ATF3, Noxa, DAPK2, and PARP cleavage in LNCaP cells, and on CG-12-induced PARP cleavage in DU-145 cells. Cells were transfected with control vector (pRS) or KLF6 shRNA (pRS-shKLF6) for 24 h and then treated with CG-12 for 48 h. ...View More
Chen, C. H., Huang, P. H., Chu, P. C., Chen, M. C., Chou, C. C., Wang, D., ... & Chen, C. S. (2011). Energy restriction-mimetic agents induce apoptosis in prostate cancer cells in part through epigenetic activation of KLF6 tumor suppressor gene expression. Journal of Biological Chemistry, 286(12), 9968-9976.

Summary

Host Animal
Mouse
Specificity
Human
Clone
6B8
Antibody Isotype
IgG3, κ
Application
ELISA, WB

Basic Information

Immunogen
ATF3 (AAH06322, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
activating transcription factor 3
Introduction
ATF3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is inv
Entrez Gene ID
467
UniProt ID
P18847
Alternative Names
Activating Transcription Factor 3; CAMP-Dependent Transcription Factor ATF-3;
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter.
Isoform 2: Activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Biological Process
Cellular response to amino acid starvation Source: Ensembl
Endoplasmic reticulum unfolded protein response Source: Ensembl
Gluconeogenesis Source: Ensembl
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of TRAIL-activated apoptotic signaling pathway Source: ParkinsonsUK-UCL
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Regulation of eIF2 alpha phosphorylation by heme Source: Reactome
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance Source: ParkinsonsUK-UCL
Skeletal muscle cell differentiation Source: Ensembl
Cellular Location
Nucleus

Ku, H. C., & Cheng, C. F. (2020). Master regulator activating transcription factor 3 (ATF3) in metabolic homeostasis and cancer. Frontiers in Endocrinology, 11.

Kole, C., Brommer, B., Nakaya, N., Sengupta, M., Bonet-Ponce, L., Zhao, T., ... & Tomarev, S. (2020). Activating transcription factor 3 (ATF3) protects retinal ganglion cells and promotes functional preservation after optic nerve crush. Investigative ophthalmology & visual science, 61(2), 31-31.

Li, X., Zang, S., Cheng, H., Li, J., & Huang, A. (2019). Overexpression of activating transcription factor 3 exerts suppressive effects in HepG2 cells. Molecular medicine reports, 19(2), 869-876.

Wu, C., Lin, H., & Zhang, X. (2019). Inhibitory effects of pirfenidone on fibroblast to myofibroblast transition in rheumatoid arthritis-associated interstitial lung disease via the downregulation of activating transcription factor 3 (ATF3). International immunopharmacology, 74, 105700.

Förstner, P., Rehman, R., Anastasiadou, S., Haffner-Luntzer, M., Sinske, D., Ignatius, A., ... & Knöll, B. (2018). Neuroinflammation after traumatic brain injury is enhanced in activating transcription factor 3 mutant mice. Journal of neurotrauma, 35(19), 2317-2329.

Rohini, M., Menon, A. H., & Selvamurugan, N. (2018). Role of activating transcription factor 3 and its interacting proteins under physiological and pathological conditions. International journal of biological macromolecules, 120, 310-317.

Zhou, H., Li, N., Yuan, Y., Jin, Y. G., Guo, H., Deng, W., & Tang, Q. Z. (2018). Activating transcription factor 3 in cardiovascular diseases: a potential therapeutic target. Basic research in cardiology, 113(5), 1-13.

Hasim, M. S., Nessim, C., Villeneuve, P. J., Vanderhyden, B. C., & Dimitroulakos, J. (2018). Activating transcription factor 3 as a novel regulator of chemotherapy response in breast cancer. Translational oncology, 11(4), 988-998.

Li, Y., Li, Z., Zhang, C., Li, P., Wu, Y., Wang, C., ... & Du, J. (2017). Cardiac Fibroblast–specific activating transcription factor 3 protects against heart failure by suppressing MAP2K3-p38 signaling. Circulation, 135(21), 2041-2057.

Li, X., Zhou, X., Li, Y., Zu, L., Pan, H., Liu, B., ... & Zhou, Q. (2017). Activating transcription factor 3 promotes malignance of lung cancer cells in vitro. Thoracic cancer, 8(3), 181-191.

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For research use only. Not intended for any clinical use.

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