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Mouse Anti-ATF7IP Recombinant Antibody (CBFYM-0870) (CBMAB-M1017-FY)

This product is mouse antibody that recognizes ATF7IP. The antibody CBFYM-0870 can be used for immunoassay techniques such as: WB.
See all ATF7IP antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYM-0870
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1058-1161 mapping near the C-terminus of ATF-7IP of mouse origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
N-terminus

Target

Full Name
ACTIVATING TRANSCRIPTION FACTOR 7 INTERACTING PROTEIN
Introduction
ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners.
Entrez Gene ID
55729
UniProt ID
Q6VMQ6
Alternative Names
Activating Transcription Factor 7 Interacting Protein; MBD1-Containing Chromatin-Associated Factor 1; ATFa-Associated Modulator; ATF7-Interacting Protein; ATF-Interacting Protein; ATF-IP; MCAF1
Function
Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed:27732843).
Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1 (PubMed:12665582).
Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) (PubMed:14536086, PubMed:27732843).
Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells (PubMed:19106100).
Biological Process
DNA methylation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of protein stability Source: UniProtKB
Regulation of RNA polymerase II transcription preinitiation complex assembly Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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