Mouse Anti-ATF7IP Recombinant Antibody (CBYC-A846) (CBMAB-A3888-YC)

Name: Mouse Anti-ATF7IP Recombinant Antibody (CBYC-A846)
SKU: CBMAB-A3888-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A846

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1058-1161 mapping near the C-terminus of ATF-7IP of mouse origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ACTIVATING TRANSCRIPTION FACTOR 7 INTERACTING PROTEIN

Introduction

ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners.

Entrez Gene ID

55729

UniProt ID

Q6VMQ6

Alternative Names

Activating Transcription Factor 7 Interacting Protein; MBD1-Containing Chromatin-Associated Factor 1; ATFa-Associated Modulator; ATF7-Interacting Protein; ATF-Interacting Protein; ATF-IP; MCAF1;

Function

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed:27732843).
Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1 (PubMed:12665582).
Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) (PubMed:14536086, PubMed:27732843).
Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells (PubMed:19106100).

Biological Process

DNA methylation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of protein stability Source: UniProtKB
Regulation of RNA polymerase II transcription preinitiation complex assembly Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus

Hu, H., Khodadadi-Jamayran, A., Dolgalev, I., Cho, H., Badri, S., Chiriboga, L. A., ... & Wong, K. K. (2021). Targeting the Atf7ip-Setdb1 complex augments antitumor immunity by boosting tumor immunogenicity. Cancer Immunology Research.

Sin, J. H., Zuckerman, C., Cortez, J. T., Eckalbar, W. L., Erle, D. J., Anderson, M. S., & Waterfield, M. R. (2019). The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses. Journal of Experimental Medicine, 216(9), 2024-2037.

Tsusaka, T., Kikuchi, M., Shimazu, T., Suzuki, T., Sohtome, Y., Akakabe, M., ... & Shinkai, Y. (2018). Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics & chromatin, 11(1), 1-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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