Mouse Anti-ATG16L1 Recombinant Antibody (A3908) (CBMAB-A3908-YC)

Name: Mouse Anti-ATG16L1 Recombinant Antibody (A3908)
SKU: CBMAB-A3908-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A3908

Application

IF, WB

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:6,000
IF(ICC)	1:200-1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Autophagy Related 16 Like 1

Introduction

ATG16L1 is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease typ

Entrez Gene ID

55054

UniProt ID

Q676U5

Alternative Names

Autophagy Related 16 Like 1; APG16L; ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae); ATG16 Autophagy Related 16-Like (S. Cerevisiae); APG16 Autophagy 16-Like (S. Cerevisiae); ATG16 Autophagy Related 16-Like 1; Autophagy-Related Protein 16-1; WD Repeat

Function

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225).
Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662).
Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340).
Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199).
Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).

Biological Process

Autophagosome assembly Source: GO_Central
Macroautophagy Source: Reactome
Negative stranded viral RNA replication Source: GO_Central
Protein transport Source: UniProtKB-KW

Cellular Location

Cytoplasm; Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.

Involvement in disease

Inflammatory bowel disease 10 (IBD10): A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

PTM

Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.

El-Refai, S. A., Helwa, M. A., Rakha, E. B., & Atia, A. F. (2021). Autophagy-related 16-like 1 single nucleotide gene polymorphism increases the risk and severity of Cryptosporidium parvum infection. Molecular and Biochemical Parasitology, 245, 111407.

Kanda, T., Yoshida, A., Ikebuchi, Y., Ikeda, H., Sakaguchi, T., Urabe, S., ... & Isomoto, H. (2021). Autophagy‑related 16‑like 1 is influenced by human herpes virus 1‑encoded microRNAs in biopsy samples from the lower esophageal sphincter muscle during per‑oral endoscopic myotomy for esophageal achalasia. Biomedical reports, 14(1), 1-1.

Wang, S. P., Li, W., Li, C., Duan, X. Y., & Duan, J. (2020). Effect of rs4719839 polymorphism on risk of ventilator‐associated pneumonia, expression of microRNA‐148 and autophagy‐related 16‐like 1 (ATG16L1). Journal of Cellular and Molecular Medicine, 24(21), 12599-12607.

Ghavami, S. B., Kabiri, F., Nourian, M., Balaii, H., Shahrokh, S., Chaleshi, V., ... & Aghdaei, H. A. (2019). Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues. Gastroenterology and hepatology from bed to bench, 12(Suppl1), S94.

Liassides, C., Papadopoulos, A., Siristatidis, C., Damoraki, G., Liassidou, A., Chrelias, C., ... & Giamarellos-Bourboulis, E. J. (2019). Single nucleotide polymorphisms of toll-like receptor-4 and of autophagy-related gene 16 like-1 gene for predisposition of premature delivery: a prospective study. Medicine, 98(40).

Li, Y., Zhou, D., Ren, Y., Zhang, Z., Guo, X., Ma, M., ... & Zhang, R. (2019). Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1. Autophagy, 15(3), 478-492.

Palanisamy, K., Tsai, T. H., Yu, T. M., Sun, K. T., Yu, S. H., Lin, F. Y., ... & Li, C. Y. (2019). RNA‐binding protein, human antigen R regulates hypoxia‐induced autophagy by targeting ATG7/ATG16L1 expressions and autophagosome formation. Journal of cellular physiology, 234(5), 7448-7458.

Zhang, K., Chen, J., Zhou, H., Chen, Y., Zhi, Y., Zhang, B., ... & Zhang, C. (2018). PU. 1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell death & disease, 9(3), 1-16.

Li, X., Chen, M., Zhang, X., Wang, M., Yang, X., Xia, Q., ... & Pan, F. (2018). Single nucleotide polymorphisms of autophagy‐related 16‐like 1 gene are associated with ankylosing spondylitis in females: a case–control study. International journal of rheumatic diseases, 21(1), 322-329.

Chen, R., Li, X., He, B., & Hu, W. (2017). MicroRNA-410 regulates autophagy-related gene ATG16L1 expression and enhances chemosensitivity via autophagy inhibition in osteosarcoma. Molecular medicine reports, 15(3), 1326-1334.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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