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Rabbit Anti-ATG16L1 Recombinant Antibody (CBYC-A851) (CBMAB-A3911-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 16 Like 1. The antibody can be used for immunoassay techniques, such as ELISA, ICC, WB.
See all ATG16L1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
CBYC-A851
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 6-105 of human ATG16L1.
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 16 Like 1
Introduction
ATG16L1 is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease typ
Entrez Gene ID
Human55054
Mouse77040
Rat363278
UniProt ID
HumanQ676U5
MouseQ8C0J2
RatA0A0G2K9U6
Alternative Names
Autophagy Related 16 Like 1; APG16L; ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae); ATG16 Autophagy Related 16-Like (S. Cerevisiae); APG16 Autophagy 16-Like (S. Cerevisiae); ATG16 Autophagy Related 16-Like 1; Autophagy-Related Protein 16-1; WD Repeat
Function
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225).
Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662).
Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340).
Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199).
Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).
Biological Process
Autophagosome assembly Source: GO_Central
Macroautophagy Source: Reactome
Negative stranded viral RNA replication Source: GO_Central
Protein transport Source: UniProtKB-KW
Cellular Location
Cytoplasm; Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
Involvement in disease
Inflammatory bowel disease 10 (IBD10): A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
PTM
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.

El-Refai, S. A., Helwa, M. A., Rakha, E. B., & Atia, A. F. (2021). Autophagy-related 16-like 1 single nucleotide gene polymorphism increases the risk and severity of Cryptosporidium parvum infection. Molecular and Biochemical Parasitology, 245, 111407.

Kanda, T., Yoshida, A., Ikebuchi, Y., Ikeda, H., Sakaguchi, T., Urabe, S., ... & Isomoto, H. (2021). Autophagy‑related 16‑like 1 is influenced by human herpes virus 1‑encoded microRNAs in biopsy samples from the lower esophageal sphincter muscle during per‑oral endoscopic myotomy for esophageal achalasia. Biomedical reports, 14(1), 1-1.

Wang, S. P., Li, W., Li, C., Duan, X. Y., & Duan, J. (2020). Effect of rs4719839 polymorphism on risk of ventilator‐associated pneumonia, expression of microRNA‐148 and autophagy‐related 16‐like 1 (ATG16L1). Journal of Cellular and Molecular Medicine, 24(21), 12599-12607.

Ghavami, S. B., Kabiri, F., Nourian, M., Balaii, H., Shahrokh, S., Chaleshi, V., ... & Aghdaei, H. A. (2019). Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues. Gastroenterology and hepatology from bed to bench, 12(Suppl1), S94.

Liassides, C., Papadopoulos, A., Siristatidis, C., Damoraki, G., Liassidou, A., Chrelias, C., ... & Giamarellos-Bourboulis, E. J. (2019). Single nucleotide polymorphisms of toll-like receptor-4 and of autophagy-related gene 16 like-1 gene for predisposition of premature delivery: a prospective study. Medicine, 98(40).

Li, Y., Zhou, D., Ren, Y., Zhang, Z., Guo, X., Ma, M., ... & Zhang, R. (2019). Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1. Autophagy, 15(3), 478-492.

Palanisamy, K., Tsai, T. H., Yu, T. M., Sun, K. T., Yu, S. H., Lin, F. Y., ... & Li, C. Y. (2019). RNA‐binding protein, human antigen R regulates hypoxia‐induced autophagy by targeting ATG7/ATG16L1 expressions and autophagosome formation. Journal of cellular physiology, 234(5), 7448-7458.

Zhang, K., Chen, J., Zhou, H., Chen, Y., Zhi, Y., Zhang, B., ... & Zhang, C. (2018). PU. 1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib. Cell death & disease, 9(3), 1-16.

Li, X., Chen, M., Zhang, X., Wang, M., Yang, X., Xia, Q., ... & Pan, F. (2018). Single nucleotide polymorphisms of autophagy‐related 16‐like 1 gene are associated with ankylosing spondylitis in females: a case–control study. International journal of rheumatic diseases, 21(1), 322-329.

Chen, R., Li, X., He, B., & Hu, W. (2017). MicroRNA-410 regulates autophagy-related gene ATG16L1 expression and enhances chemosensitivity via autophagy inhibition in osteosarcoma. Molecular medicine reports, 15(3), 1326-1334.

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For research use only. Not intended for any clinical use.

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