Mouse Anti-ATG4C Recombinant Antibody (CBT2187) (V2LY-0625-LY757)

Name: Mouse Anti-ATG4C Recombinant Antibody (CBT2187)
SKU: V2LY-0625-LY757
Rating: 5 (1 reviews)

See all ATG4C antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT2187

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Purified recombinant fragment of human ATG4C (AA: 321-458) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Autophagy Related 4C Cysteine Peptidase

Entrez Gene ID

84938

UniProt ID

Q96DT6

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Biological Process

Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

More Infomation

References

Wen, Z. P., Zeng, W. J., Chen, Y. H., Li, H., Wang, J. Y., Cheng, Q., ... & Chen, X. P. (2019). Knockdown ATG4C inhibits gliomas progression and promotes temozolomide chemosensitivity by suppressing autophagic flux. Journal of Experimental & Clinical Cancer Research, 38(1), 1-15.

Antonelli, M., Strappazzon, F., Arisi, I., Brandi, R., D’Onofrio, M., Sambucci, M., ... & Stagni, V. (2017). ATM kinase sustains breast cancer stem-like cells by promoting ATG4C expression and autophagy. Oncotarget, 8(13), 21692.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-ATG4C Recombinant Antibody (CBT2187)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ATG4C Recombinant Antibody (12.2.2)

Rabbit Anti-ATG4C Recombinant Antibody (EG294)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: