Mouse Anti-ATG4C Recombinant Antibody (CBT2187) (V2LY-0625-LY757)

Name: Mouse Anti-ATG4C Recombinant Antibody (CBT2187)
SKU: V2LY-0625-LY757
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ATG4C Recombinant Antibody (CBT2187) (V2LY-0625-LY757) in ELISA

Mouse Anti-ATG4C Recombinant Antibody (CBT2187) (V2LY-0625-LY757) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2187

Application

Immunogen

Purified recombinant fragment of human ATG4C (AA: 321-458) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Autophagy Related 4C Cysteine Peptidase

Entrez Gene ID

84938

UniProt ID

Q96DT6

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Biological Process

Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB

Cellular Location

Cytoplasm

Wen, Z. P., Zeng, W. J., Chen, Y. H., Li, H., Wang, J. Y., Cheng, Q., ... & Chen, X. P. (2019). Knockdown ATG4C inhibits gliomas progression and promotes temozolomide chemosensitivity by suppressing autophagic flux. Journal of Experimental & Clinical Cancer Research, 38(1), 1-15.

Antonelli, M., Strappazzon, F., Arisi, I., Brandi, R., D’Onofrio, M., Sambucci, M., ... & Stagni, V. (2017). ATM kinase sustains breast cancer stem-like cells by promoting ATG4C expression and autophagy. Oncotarget, 8(13), 21692.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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