Mouse Anti-ATP10A Recombinant Antibody (G-9) (CBMAB-A3999-YC)

Name: Mouse Anti-ATP10A Recombinant Antibody (G-9)
SKU: CBMAB-A3999-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

G-9

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1001-1064 mapping near the C-terminus of ATP10A of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATPase Phospholipid Transporting 10A (Putative)

Introduction

ATP10A belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to ano

Entrez Gene ID

Human	57194
Mouse	11982
Rat	365266

UniProt ID

Human	O60312
Mouse	O54827
Rat	A0A0G2JWQ8

Alternative Names

ATPase Phospholipid Transporting 10A (Putative); ATP10C; P4-ATPase Flippase Complex Alpha Subunit ATP10A; Aminophospholipid Translocase VA; EC 3.6.3.1; ATPVA; ATPVC; ATPase Type IV, Phospholipid Transporting (P-Type);

Function

Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane (PubMed:25947375, PubMed:29599178, PubMed:30530492).
Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking (PubMed:29599178).
Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix (PubMed:29599178, PubMed:25947375).
Has low flippase activity toward glucosylceramide (GlcCer) (PubMed:30530492).

Biological Process

Ion transmembrane transport Source: Reactome
Phospholipid translocation Source: GO_Central
Positive regulation of membrane tubulation Source: UniProtKB
Regulation of cell shape Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Cell membrane. Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not that of TMEM30B.

Topology

Cytoplasmic: 1-86 aa
Helical: 87-106 aa
Exoplasmic loop: 107-110 aa
Helical: 111-128 aa
Cytoplasmic: 129-309 aa
Helical: 310-332 aa
Exoplasmic loop: 333-362 aa
Helical: 363-384 aa
Cytoplasmic: 385-1087 aa
Helical: 1088-1108 aa
Exoplasmic loop: 1109-1119 aa
Helical: 1120-1140 aa
Cytoplasmic: 1141-1170 aa
Helical: 1171-1192 aa
Exoplasmic loop: 1193-1199 aa
Helical: 1200-1222 aa
Cytoplasmic: 1223-1228 aa
Helical: 1229-1249 aa
Exoplasmic loop: 1250-1267 aa
Helical: 1268-1292 aa
Cytoplasmic: 1293-1499 aa

PTM

Autophosphorylated at the conserved aspartate of the P-type ATPase signature sequence.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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