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Mouse Anti-ATP1A1 Recombinant Antibody (C464.6) (CBMAB-N0624-WJ)

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Basic Information

Host Animal
Mouse
Clone
C464.6
Application
ICC, IF, IHC-P, IP, WB
Immunogen
Purified rabbit renal outer medulla.
Specificity
Dog, Human, Monkey, Mouse, Pig, Rat, Frog
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
ATPase Na+/K+ Transporting Subunit Alpha 1
Introduction
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Entrez Gene ID
Human476
Mouse11928
Rat24211
Dog403992
Pig397481
Frog395044
Monkey710368
UniProt ID
HumanP05023
MouseQ8VDN2
RatP06685
DogP50997
PigP05024
FrogQ92123
MonkeyA0A1U7QLM8
Alternative Names
ATPase Na+/K+ Transporting Subunit Alpha 1; Sodium Pump Subunit Alpha-1; EC 3.6.3.9; Sodium/Potassium-Transporting ATPase Subunit Alpha-1; Sodium-Potassium ATPase Catalytic Subunit Alpha-1; ATPase, Na+/K+ Transporting, Alpha 1 Polypeptide; Na(+)/K(+) ATPase Alpha-1 Subunit; EC 3.6.3;
Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Biological Process
Cardiac muscle cell action potential involved in contraction Source: BHF-UCL
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular response to mechanical stimulus Source: Ensembl
Cellular response to steroid hormone stimulus Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Membrane hyperpolarization Source: Ensembl
Membrane repolarization Source: BHF-UCL
Membrane repolarization during cardiac muscle cell action potential Source: BHF-UCL
Negative regulation of glucocorticoid biosynthetic process Source: Ensembl
Negative regulation of heart contraction Source: Ensembl
Positive regulation of heart contraction Source: Ensembl
Positive regulation of striated muscle contraction Source: Ensembl
Potassium ion import across plasma membrane Source: BHF-UCL
Proton transmembrane transport Source: GO_Central
Regulation of blood pressure Source: Ensembl
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle cell contraction Source: Ensembl
Regulation of sodium ion transport Source: UniProtKB
Regulation of the force of heart contraction Source: Ensembl
Relaxation of cardiac muscle Source: BHF-UCL
Response to drug Source: Ensembl
Response to glycoside Source: BHF-UCL
Sodium ion export across plasma membrane Source: BHF-UCL
Cellular Location
Basolateral cell membrane; Sarcolemma; Axon; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Charcot-Marie-Tooth disease 2DD (CMT2DD): A dominant axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Hypomagnesemia, seizures, and mental retardation 2 (HOMGSMR2): An autosomal dominant disease characterized by generalized seizures in infancy, severe hypomagnesemia, and renal magnesium wasting. Seizures persist despite magnesium supplementation and are associated with significant intellectual disability.
Topology
Cytoplasmic: 6-87 aa
Helical: 88-108 aa
Extracellular: 109-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-288 aa
Helical: 289-308 aa
Extracellular: 309-320 aa
Helical: 321-338 aa
Cytoplasmic: 339-772 aa
Helical: 773-792 aa
Extracellular: 793-802 aa
Helical: 803-823 aa
Cytoplasmic: 824-843 aa
Helical: 844-866 aa
Extracellular: 867-918 aa
Helical: 919-938 aa
Cytoplasmic: 939-951 aa
Helical: 952-970 aa
Extracellular: 971-985 aa
Helical: 986-1006 aa
Cytoplasmic: 1007-1023 aa
PTM
Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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