Sign in or Register   Sign in or Register
  |  

Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178) (CBMAB-1162-CN)

This product is a mouse antibody that recognizes ATP1A1 of human. The antibody CBLNA-178 can be used for immunoassay techniques such as: WB.
See all ATP1A1 antibodies
Published Data
Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178) (CBMAB-1162-CN) in IF
(A) Schematic diagram depicting the expansion strategy of human CECs using a dual media approach, inter-switching between a M5-Endo medium for cellular stabilization/maintenance and a M4-F99 medium for cell proliferation. (B) Representative micrograph of a confluent homogeneous monolayer of P0 human CECs maintained for several days in M5-Endo medium following proliferative expansion in M4-F99 medium. Representative micrographic indirect immuno-fluorescent images of human CECs taken at the first passage which express (C) NA1/K1-ATPase, (D) ZO-1, (E) GPC-4 and (F) CD-200, indicative of the corneal endothelium. Scale Bars - B: 100 mm; C-F: 50 mm ...View More
Peh, G. S., Adnan, K., George, B. L., Ang, H. P., Seah, X. Y., Tan, D. T., & Mehta, J. S. (2015). The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach. Scientific reports, 5(1), 9167.
Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178) (CBMAB-1162-CN) in WB
Expression of Na,K-ATPase α- and β-subunit isoforms in cerebella of C57BL/6J mice at indicated ages, α-tubulin served as a loading control. ...View More
Litan, A., Li, Z., Tokhtaeva, E., Kelly, P., Vagin, O., & Langhans, S. A. (2019). A functional interaction between Na, K-ATPase β 2-subunit/AMOG and NF2/merlin regulates growth factor signaling in cerebellar granule cells. Molecular neurobiology, 56, 7557-7571.
Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178) (CBMAB-1162-CN) in IP
(A) Primary cultures of human CEnCs) were treated with tert-butyl hydroperoxide (tBHP) for 3 h. pCEnCs were biotinylated and cell surface proteins purified by immunoprecipitation. The flow-through, unbound fraction served as a control for membrane fractionation. (B) pCEnCs and (C) A549 cells were treated with tBHP for 3 h and plasma membrane was isolated by density dependent centrifugation. Data from three independent experiments ± SEM is shown. Prdx6 levels were normalized to Na+/K+-ATPase and expressed relative to untreated (−) controls. Student t-test was performed to evaluate statistical significance (** p-value < 0.005). ...View More
Lovatt, M., Adnan, K., Peh, G. S., & Mehta, J. S. (2018). Regulation of oxidative stress in corneal endothelial cells by Prdx6. Antioxidants, 7(12), 180.
Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178) (CBMAB-1162-CN) in IF
To assess the efect of storage duration, cells were stored in Endo-SFM(+) at 4 °C and viability was assessed with Annexin V/propidium iodide at several time points (pre-storage, 2 days, 4 days, and 6 days). HCEnCs were either placed directly into storage, or subject to simulated transportation on a shaker plate for the frst 2 days. Statistical signifcance was determined via Dunnett's post-hoc test with pre-storage viability as the control. (*p<0.05, **p<0.01; n=3). ...View More
Wahlig, S., Peh, G. S., Adnan, K., Ang, H. P., Lwin, C. N., Morales-Wong, F., ... & Mehta, J. S. (2020). Optimisation of storage and transportation conditions of cultured corneal endothelial cells for cell replacement therapy. Scientific Reports, 10(1), 1681.

Summary

Host Animal
Mouse
Specificity
Dog, Human, Monkey, Mouse, Pig, Rat, Frog
Clone
CBLNA-178
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Purified rabbit renal outer medulla.
Specificity
Dog, Human, Monkey, Mouse, Pig, Rat, Frog
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase Na+/K+ Transporting Subunit Alpha 1
Introduction
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Entrez Gene ID
476
UniProt ID
P05023
Alternative Names
sodium/potassium-transporting ATPase subunit alpha-1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase 1; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; EC 3.6.3.9
Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Biological Process
Cardiac muscle cell action potential involved in contraction Source: BHF-UCL
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular response to mechanical stimulus Source: Ensembl
Cellular response to steroid hormone stimulus Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Membrane hyperpolarization Source: Ensembl
Membrane repolarization Source: BHF-UCL
Membrane repolarization during cardiac muscle cell action potential Source: BHF-UCL
Negative regulation of glucocorticoid biosynthetic process Source: Ensembl
Negative regulation of heart contraction Source: Ensembl
Positive regulation of heart contraction Source: Ensembl
Positive regulation of striated muscle contraction Source: Ensembl
Potassium ion import across plasma membrane Source: BHF-UCL
Proton transmembrane transport Source: GO_Central
Regulation of blood pressure Source: Ensembl
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle cell contraction Source: Ensembl
Regulation of sodium ion transport Source: UniProtKB
Regulation of the force of heart contraction Source: Ensembl
Relaxation of cardiac muscle Source: BHF-UCL
Response to drug Source: Ensembl
Response to glycoside Source: BHF-UCL
Sodium ion export across plasma membrane Source: BHF-UCL
Cellular Location
Basolateral cell membrane; Sarcolemma; Axon; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Involvement in disease
Charcot-Marie-Tooth disease 2DD (CMT2DD): A dominant axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Hypomagnesemia, seizures, and mental retardation 2 (HOMGSMR2): An autosomal dominant disease characterized by generalized seizures in infancy, severe hypomagnesemia, and renal magnesium wasting. Seizures persist despite magnesium supplementation and are associated with significant intellectual disability.
Topology
Cytoplasmic: 6-87 aa
Helical: 88-108 aa
Extracellular: 109-131 aa
Helical: 132-152 aa
Cytoplasmic: 153-288 aa
Helical: 289-308 aa
Extracellular: 309-320 aa
Helical: 321-338 aa
Cytoplasmic: 339-772 aa
Helical: 773-792 aa
Extracellular: 793-802 aa
Helical: 803-823 aa
Cytoplasmic: 824-843 aa
Helical: 844-866 aa
Extracellular: 867-918 aa
Helical: 919-938 aa
Cytoplasmic: 939-951 aa
Helical: 952-970 aa
Extracellular: 971-985 aa
Helical: 986-1006 aa
Cytoplasmic: 1007-1023 aa
PTM
Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ATP1A1 Recombinant Antibody (CBLNA-178)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare