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Mouse Anti-ATP1A3 Recombinant Antibody (CBWJN-0809) (CBMAB-N0628-WJ)

This product is a Mouse antibody that recognizes ATP1A3. The antibody CBWJN-0809 can be used for immunoassay techniques such as: IHC, WB.
See all ATP1A3 antibodies
Published Data
Mouse Anti-ATP1A3 Recombinant Antibody (CBWJN-0809) (CBMAB-N0628-WJ) in WB
Western blotting results of Na, K-ATPase α1 and α3 in HeLa cells treated with arenobufagin(Arg) at different doses for 24 h. ...View More
Yue, Q., Zhen, H., Huang, M., Zheng, X., Feng, L., Jiang, B., ... & Guo, D. (2016). Proteasome inhibition contributed to the cytotoxicity of arenobufagin after its binding with Na, K-ATPase in human cervical carcinoma HeLa cells. PloS one, 11(7), e0159034.

Summary

Host Animal
Mouse
Specificity
Human, Rat, Mouse
Clone
CBWJN-0809
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-65 mapping at the N-terminus of Na+/K+-ATPase α3 of human origin.
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase Na+/K+ Transporting Subunit Alpha 3
Introduction
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Entrez Gene ID
Human478
Rat24213
Dog612463
Sheep101103751
Rabbit100349382
Monkey706513
UniProt ID
HumanP13637
RatP06687
DogF1P767
SheepW5P3P0
RabbitG1TZA0
MonkeyA0A1D5QT51
Alternative Names
ATPase Na+/K+ Transporting Subunit Alpha 3; Sodium Pump Subunit Alpha-3; Sodium/Potassium-Transporting ATPase Subunit Alpha-3; Sodium-Potassium ATPase Catalytic Subunit Alpha-3; ATPase, Na+/K+ Transporting, Alpha 3 Polypeptide; Na(+)/K(+) ATPase Alpha(III) Subunit; Na(+)/K(+) ATPase Alpha-3 Subunit; EC 3.6.3.9; Na+, K+ Activated Adenosine Triphosphatase Alpha Subunit; Sodium/Potassium-Transporting ATPase Alpha-3 Chain;
Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Biological Process
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular response to amyloid-beta Source: ARUK-UCL
Cellular response to steroid hormone stimulus Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Neuron projection maintenance Source: ARUK-UCL
Potassium ion import across plasma membrane Source: BHF-UCL
Proton transmembrane transport Source: GO_Central
Regulation of cardiac conduction Source: Reactome
Regulation of resting membrane potential Source: ARUK-UCL
Response to glycoside Source: BHF-UCL
Sodium ion export across plasma membrane Source: BHF-UCL
Cellular Location
Cell membrane
Involvement in disease
Dystonia 12 (DYT12): An autosomal dominant dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT12 patients develop dystonia and parkinsonism between 15 and 45 years of age. The disease is characterized by an unusually rapid evolution of signs and symptoms. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
Alternating hemiplegia of childhood 2 (AHC2): A rare syndrome of episodic hemi- or quadriplegia lasting minutes to days. Most cases are accompanied by dystonic posturing, choreoathetoid movements, nystagmus, other ocular motor abnormalities, autonomic disturbances, and progressive cognitive impairment. It is typically distinguished from familial hemiplegic migraine by infantile onset and high prevalence of associated neurological deficits that become increasingly obvious with age.
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss (CAPOS): An autosomal dominant neurologic disorder characterized by relapsing and partially remitting, early-onset cerebellar ataxia following a febrile illness. Other features include progressive optic atrophy and sensorineural hearing loss, generalized hypotonia, areflexia and pes cavus without evidence of a peripheral neuropathy on neurophysiological studies.
Topology
Cytoplasmic: 1-77 aa
Helical: 78-98 aa
Extracellular: 99-121 aa
Helical: 122-142 aa
Cytoplasmic: 143-278 aa
Helical: 279-298 aa
Extracellular: 299-310 aa
Helical: 311-328 aa
Cytoplasmic: 329-762 aa
Helical: 763-782 aa
Extracellular: 783-792 aa
Helical: 793-813 aa
Cytoplasmic: 814-833 aa
Helical: 834-856 aa
Extracellular: 857-908 aa
Helical: 909-928 aa
Cytoplasmic: 929-941 aa
Helical: 942-960 aa
Extracellular: 961-975 aa
Helical: 976-996 aa
Cytoplasmic: 997-1013 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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