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Mouse Anti-ATP4B Recombinant Antibody (2G11) (CBMAB-A4039-YC)

Provided herein is a Mouse monoclonal antibody against Pig ATPase H+/K+ Transporting Beta Subunit. The antibody can be used for immunoassay techniques, such as ChIP, FC.
See all ATP4B antibodies
Published Data
Mouse Anti-ATP4B Recombinant Antibody (2G11) (CBMAB-A4039-YC) in Immunoblot
Immunoblotting for ATP4B and ZnT3-HA in anti-HA or anti-ATP4B antibody immunoprecipitates prepared from astrocytes transfected with ZnT3-HA for 72 hr. ATP4B was co-immunoprecipitated with ZnT3-HA (upper panels) and ZnT3-HA was co-immunoprecipitated with ATP4B (lower panels). Arrowheads show specific signals detected by each antibody and asterisk indicates the light chain of immunoglobulin. Experiments were repeated at least five times and representative images were shown. ...View More
Lee, H., & Koh, J. Y. (2021). Roles for H+/K+-ATPase and zinc transporter 3 in cAMP-mediated lysosomal acidification in bafilomycin A1-treated astrocytes. Glia, 69(5), 1110-1125.
Mouse Anti-ATP4B Recombinant Antibody (2G11) (CBMAB-A4039-YC) in IHC
Comparison of gene expression between two subtypes of EPN-PF. a Representative photomicrographs stained with ATP4B. The expression of ATP4B in EPN-PFB was signifcantly higher than that in EPN-PFA (P=0.0363). b Representative photomicrographs stained with CCDC151. The expression of CCDC151 in EPN-PFB was signifcantly higher than that in EPN-PFA (P=0.0280). c Representative photomicrographs stained with DMKN. The expression of DMKN in EPN-PFB was signifcantly higher than that in EPN-PFA (P=0.0003). d Representative photomicrographs stained with SCN4B.The expression of SCN4B in EPN-PFB was higher than that in EPN-PF, but the diference was no statistically signifcant (P=0.0363). ...View More
Wang, G., Jia, Y., Ye, Y., Kang, E., Chen, H., Wang, J., & He, X. (2021). Identification of key methylation differentially expressed genes in posterior fossa ependymoma based on epigenomic and transcriptome analysis. Journal of Translational Medicine, 19, 1-14.
Mouse Anti-ATP4B Recombinant Antibody (2G11) (CBMAB-A4039-YC) in FC
Quantification of flow cytometric data. ...View More
Bertaux-Skeirik, N., Feng, R., Schumacher, M. A., Li, J., Mahe, M. M., Engevik, A. C., ... & Zavros, Y. (2015). CD44 plays a functional role in Helicobacter pylori-induced epithelial cell proliferation. PLoS pathogens, 11(2), e1004663.
Fig.4 Immunolocalization of H+/K+-ATPases in Capan-1 cells grown on permeable membranes.
...View More
Fig.5 Immunolocalization of H+/K+-ATPases in sections of human pancreas.
...View More

Summary

Host Animal
Mouse
Specificity
Cattle, Dog, Human, Mouse, Pig, Rabbit, Rat
Clone
2G11
Antibody Isotype
IgG1
Application
IHC, IF, IP, WB, FC

Basic Information

Immunogen
Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.
Specificity
Cattle, Dog, Human, Mouse, Pig, Rabbit, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:4,000
IHC-P1:2,000
FC1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+/K+ exchanging, beta polypeptide
Introduction
ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump tha
Entrez Gene ID
397131
UniProt ID
P18434
Alternative Names
ATPase H+/K+ Transporting Beta Subunit; ATPase, H+/K+ Exchanging, Beta Polypeptide; Gastric H(+)/K(+) ATPase Subunit Beta; Proton Pump Beta Chain; ATPase, H+/K+ Transporting, Beta Polypeptide; Potassium-Transporting ATPase Subunit Beta;
Function
The beta subunit of the gastric H+/K+ ATPase pump which transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity).
Within a transport cycle, the transfer of a H+ ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity).
Biological Process
Cell adhesion Source: UniProtKB-KW
Cellular potassium ion homeostasis Source: GO_Central
Cellular sodium ion homeostasis Source: GO_Central
Ion transmembrane transport Source: Reactome
pH reduction Source: Ensembl
Potassium ion import across plasma membrane Source: GO_Central
Response to lipopolysaccharide Source: Ensembl
Response to organonitrogen compound Source: Ensembl
Sodium ion export across plasma membrane Source: GO_Central
Cellular Location
Apical cell membrane; Cell membrane. Localized in the apical canalicular membrane of parietal cells.
Topology
Cytoplasmic: 1-36 aa
Helical: 37-57 aa
Extracellular: 58-291 aa
PTM
N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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