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Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY)

This product is mouse antibody that recognizes ATP4B. The antibody CBFYH-0030 can be used for immunoassay techniques such as: ICC, IHC-Fr, IHC-P, IP, WB, FuncS.
See all ATP4B antibodies
Published Data
Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF
BChE+ mouse gastric cells. (a-c) Representative confocal microscopy images of the glandular stomach in the corpus region showing BChE+ cells (red) that also express H+,K+-ATPase, a widely used marker of mouse gastric parietal cells (green). (d-f) At higher magnification, nearly all BChE+ cells (red) are also H+,K+-ATPase-positive (green). (g-i) Representative confocal images from the glandular stomach in the corpus region showing BChE+ cells (red) that are negative for, but very close, to cells positive for GIF, a widely used marker of mouse gastric chief cells (green). (j-l) Representative confocal images of the glandular stomach in the corpus region showing BChE+ cells (red) that are negative for, but in close proximity to, cells positive for CgA, a widely used marker of mouse gastric neuroendocrine cells (green). ...View More
Severi, I., Abbatelli, S., Perugini, J., Di Mercurio, E., Senzacqua, M., & Giordano, A. (2023). Butyrylcholinesterase distribution in the mouse gastrointestinal tract: An immunohistochemical study. Journal of Anatomy, 242(2), 245-256.
Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF
Fluorescent immunohistochemistry of parietal cell marker Hþ/Kþ adenosine triphosphatase (ATPase) (green, clone C-4) performed on FFPE sections. Nuclei were stained with Hoechst (blue). The staining shows depletion of Hþ/Kþ ATPase, which is consistent with the loss of parietal cells on H&E. Hþ/Kþ ATPase staining was replicated 3 independent times with 2 inflamed controls and 2 noninflamed controls. Inflamed control shown in the figure is positive for H pylori. Lysozyme staining was replicated in at least 3 independent experiments including a total of 3 noninflamed controls and 4 inflamed controls. Identical
thresholding parameters for each marker were used. ...View More
Al-Shaibi, A. A., Abdel-Motal, U. M., Hubrack, S. Z., Bullock, A. N., Al-Marri, A. A., Agrebi, N., ... & Lo, B. (2021). Human AGR2 deficiency causes mucus barrier dysfunction and infantile inflammatory bowel disease. Cellular and Molecular Gastroenterology and Hepatology, 12(5), 1809-1830.
Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in Immunoblot
Immunoblots revealing H+/K+-ATPase-β expression. Cancer cell line proteins were separated on an 11.25% SDS‑PAGE gel and probed with anti‑H+/K+-ATPase-β Ab. ...View More
Takeda, A., Takano, N., Kokuba, H., Hino, H., Moriya, S., Abe, A., ... & Miyazawa, K. (2020). Macrolide antibiotics enhance the antitumor effect of lansoprazole resulting in lysosomal membrane permeabilization‑associated cell death. International Journal of Oncology, 57(6), 1280-1292.
Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF
Representative immunofluorescence image from corpus gland in Cck2r-CreERT;R26- tdTomato mice at 24h after TAM, showing abundant single recombined cells (in red), with immunostaining for H/K-ATPase-expressing parietal cells shown in green. Yellow arrows indicate Cck2r-expressing parietal cells. ...View More
Chang, W., Wang, H., Kim, W., Liu, Y., Deng, H., Liu, H., ... & Wang, T. C. (2020). Hormonal suppression of stem cells inhibits symmetric cell division and gastric tumorigenesis. Cell Stem Cell, 26(5), 739-754.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYH-0030
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Peptide mapping within an extracellular domain of H+/K+ ATPase β of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+/K+ exchanging, beta polypeptide
Introduction
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
Entrez Gene ID
Human496
Mouse11945
Rat24217
Pig397131
Cattle614308
Rabbit100009125
Dog404020
UniProt ID
HumanP51164
MouseP50992
RatP18598
PigP18434
CattleE1BLX8
RabbitP18597
DogP33704
Alternative Names
ATPase H+/K+ Transporting Beta Subunit; ATPase, H+/K+ Exchanging, Beta Polypeptide; Gastric H(+)/K(+) ATPase Subunit Beta; Proton Pump Beta Chain; ATPase, H+/K+ Transporting, Beta Polypeptide; Potassium-Transporting ATPase Subunit Beta
Function
The beta subunit of the gastric H+/K+ ATPase pump which transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity).
Within a transport cycle, the transfer of a H+ ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity).
Biological Process
Cell adhesion Source: UniProtKB-KW
Cellular potassium ion homeostasis Source: GO_Central
Cellular sodium ion homeostasis Source: GO_Central
Ion transmembrane transport Source: Reactome
pH reduction Source: Ensembl
Potassium ion import across plasma membrane Source: GO_Central
Response to lipopolysaccharide Source: Ensembl
Response to organonitrogen compound Source: Ensembl
Sodium ion export across plasma membrane Source: GO_Central
Cellular Location
Apical cell membrane; Cell membrane. Localized in the apical canalicular membrane of parietal cells.
Topology
Cytoplasmic: 1-36 aa
Helical: 37-57 aa
Extracellular: 58-291 aa
PTM
N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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