Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY)

Name: Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030)
SKU: CBMAB-H0840-FY
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Published Data

Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF

BChE+ mouse gastric cells. (a-c) Representative confocal microscopy images of the glandular stomach in the corpus region showing BChE+ cells (red) that also express H+,K+-ATPase, a widely used marker of mouse gastric parietal cells (green). (d-f) At higher magnification, nearly all BChE+ cells (red) are also H+,K+-ATPase-positive (green). (g-i) Representative confocal images from the glandular stomach in the corpus region showing BChE+ cells (red) that are negative for, but very close, to cells positive for GIF, a widely used marker of mouse gastric chief cells (green). (j-l) Representative confocal images of the glandular stomach in the corpus region showing BChE+ cells (red) that are negative for, but in close proximity to, cells positive for CgA, a widely used marker of mouse gastric neuroendocrine cells (green). ...View More

Severi, I., Abbatelli, S., Perugini, J., Di Mercurio, E., Senzacqua, M., & Giordano, A. (2023). Butyrylcholinesterase distribution in the mouse gastrointestinal tract: An immunohistochemical study. Journal of Anatomy, 242(2), 245-256.

Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF

Fluorescent immunohistochemistry of parietal cell marker Hþ/Kþ adenosine triphosphatase (ATPase) (green, clone C-4) performed on FFPE sections. Nuclei were stained with Hoechst (blue). The staining shows depletion of Hþ/Kþ ATPase, which is consistent with the loss of parietal cells on H&E. Hþ/Kþ ATPase staining was replicated 3 independent times with 2 inflamed controls and 2 noninflamed controls. Inflamed control shown in the figure is positive for H pylori. Lysozyme staining was replicated in at least 3 independent experiments including a total of 3 noninflamed controls and 4 inflamed controls. Identical
thresholding parameters for each marker were used. ...View More

Al-Shaibi, A. A., Abdel-Motal, U. M., Hubrack, S. Z., Bullock, A. N., Al-Marri, A. A., Agrebi, N., ... & Lo, B. (2021). Human AGR2 deficiency causes mucus barrier dysfunction and infantile inflammatory bowel disease. Cellular and Molecular Gastroenterology and Hepatology, 12(5), 1809-1830.

Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in Immunoblot

Immunoblots revealing H+/K+-ATPase-β expression. Cancer cell line proteins were separated on an 11.25% SDS‑PAGE gel and probed with anti‑H+/K+-ATPase-β Ab. ...View More

Takeda, A., Takano, N., Kokuba, H., Hino, H., Moriya, S., Abe, A., ... & Miyazawa, K. (2020). Macrolide antibiotics enhance the antitumor effect of lansoprazole resulting in lysosomal membrane permeabilization‑associated cell death. International Journal of Oncology, 57(6), 1280-1292.

Mouse Anti-ATP4B Recombinant Antibody (CBFYH-0030) (CBMAB-H0840-FY) in IF

Representative immunofluorescence image from corpus gland in Cck2r-CreERT;R26- tdTomato mice at 24h after TAM, showing abundant single recombined cells (in red), with immunostaining for H/K-ATPase-expressing parietal cells shown in green. Yellow arrows indicate Cck2r-expressing parietal cells. ...View More

Chang, W., Wang, H., Kim, W., Liu, Y., Deng, H., Liu, H., ... & Wang, T. C. (2020). Hormonal suppression of stem cells inhibits symmetric cell division and gastric tumorigenesis. Cell Stem Cell, 26(5), 739-754.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0030

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Peptide mapping within an extracellular domain of H+/K+ ATPase β of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATPase, H+/K+ exchanging, beta polypeptide

Introduction

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

Entrez Gene ID

Human	496
Mouse	11945
Rat	24217
Pig	397131
Cattle	614308
Rabbit	100009125
Dog	404020

UniProt ID

Human	P51164
Mouse	P50992
Rat	P18598
Pig	P18434
Cattle	E1BLX8
Rabbit	P18597
Dog	P33704

Alternative Names

ATPase H+/K+ Transporting Beta Subunit; ATPase, H+/K+ Exchanging, Beta Polypeptide; Gastric H(+)/K(+) ATPase Subunit Beta; Proton Pump Beta Chain; ATPase, H+/K+ Transporting, Beta Polypeptide; Potassium-Transporting ATPase Subunit Beta

Function

The beta subunit of the gastric H+/K+ ATPase pump which transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity).
Within a transport cycle, the transfer of a H+ ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity).

Biological Process

Cell adhesion Source: UniProtKB-KW
Cellular potassium ion homeostasis Source: GO_Central
Cellular sodium ion homeostasis Source: GO_Central
Ion transmembrane transport Source: Reactome
pH reduction Source: Ensembl
Potassium ion import across plasma membrane Source: GO_Central
Response to lipopolysaccharide Source: Ensembl
Response to organonitrogen compound Source: Ensembl
Sodium ion export across plasma membrane Source: GO_Central

Cellular Location

Apical cell membrane; Cell membrane. Localized in the apical canalicular membrane of parietal cells.

Topology

Cytoplasmic: 1-36 aa
Helical: 37-57 aa
Extracellular: 58-291 aa

PTM

N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane.

Lahner, E., Marzinotto, I., Brigatti, C., Davidson, H., Wenzlau, J., Piemonti, L., ... & Lampasona, V. (2019). Measurement of autoantibodies to gastric H+, K+-ATPase (ATP4A/B) using a luciferase immunoprecipitation system (LIPS). In Autoantibodies (pp. 113-131). Humana Press, New York, NY.

Lahner, E., Brigatti, C., Marzinotto, I., Carabotti, M., Scalese, G., Davidson, H. W., ... & Lampasona, V. (2017). Luminescent immunoprecipitation system (LIPS) for detection of autoantibodies against ATP4A and ATP4B subunits of gastric proton pump H+, K+-ATPase in atrophic body gastritis patients. Clinical and translational gastroenterology, 8(1), e215.

Lin, S., Lin, B., Wang, X., Pan, Y., Xu, Q., He, J. S., ... & Huang, J. (2017). Silencing of ATP4B of ATPase H+/K+ transporting beta subunit by intragenic epigenetic alteration in human gastric cancer cells. Oncology research, 25(3), 317.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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