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Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) (CBMAB-A4062-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Synthase Inhibitory Factor Subunit 1. The antibody can be used for immunoassay techniques, such as ICC, IF, WB.
See all ATP5IF1 antibodies
Published Data
Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) in WB
Western blot analysis and quantification for the relative abundance of the fusion protein TOM20·HA and organelle-specific proteins in whole-cell lysates (W) and lysates from mitoTag immunoprecipitates (IP). ...View More
Gella, A., Prada-Dacasa, P., Carrascal, M., Urpi, A., González-Torres, M., Abian, J., ... & Quintana, A. (2020). Mitochondrial proteome of affected glutamatergic neurons in a mouse model of Leigh syndrome. Frontiers in Cell and Developmental Biology, 8, 660.
Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) in IF
BTSC73 and BTSC147 were subjected to immunostaining using antibodies to OSMR (green) and the mitochondrial matrix protein ATP synthase inhibitor F1 (ATPIF1, red). Nuclei were stained with DAPI. White rectangles mark the inset to demonstrate the co-localization of OSMR with ATPIF1. ...View More
Sharanek, A., Burban, A., Laaper, M., Heckel, E., Joyal, J. S., Soleimani, V. D., & Jahani-Asl, A. (2020). OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nature communications, 11(1), 4116.

Summary

Host Animal
Mouse
Specificity
Human, Cattle, Mouse, Rat, Dog
Clone
A4062
Antibody Isotype
IgG1, κ
Application
ICC, IF, WB, IHC

Basic Information

Immunogen
Recombinant IF1.
Specificity
Human, Cattle, Mouse, Rat, Dog
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml
IF(ICC)1-5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Synthase Inhibitory Factor Subunit 1
Introduction
ATP5IF1 is a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
Entrez Gene ID
Human93974
Mouse11983
Rat25392
Cattle327699
UniProt ID
HumanQ9UII2
MouseO35143
RatQ03344
CattleP01096
Alternative Names
ATP Synthase Inhibitory Factor Subunit 1; ATPase Inhibitory Factor 1; ATPIF1; ATP Synthase Inhibitor Protein; Inhibitor Of F(1)F(O)-ATPase; ATPase Inhibitor Protein; IF(1);
Function
Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Biological Process
Angiogenesis Source: UniProtKB
Erythrocyte differentiation Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Heme biosynthetic process Source: UniProtKB
Mitochondrial depolarization Source: ParkinsonsUK-UCL
Negative regulation of ATPase activity Source: ParkinsonsUK-UCL
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of hydrolase activity Source: UniProtKB
Positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: Ensembl
Positive regulation of proteolysis involved in cellular protein catabolic process Source: ParkinsonsUK-UCL
Reactive oxygen species metabolic process Source: Ensembl
Regulation of ATP metabolic process Source: ParkinsonsUK-UCL
Regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Cellular Location
Mitochondrion
PTM
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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