Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) (CBMAB-A4062-YC)

Name: Mouse Anti-ATP5IF1 Recombinant Antibody (A4062)
SKU: CBMAB-A4062-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) in WB

Western blot analysis and quantification for the relative abundance of the fusion protein TOM20·HA and organelle-specific proteins in whole-cell lysates (W) and lysates from mitoTag immunoprecipitates (IP). ...View More

Gella, A., Prada-Dacasa, P., Carrascal, M., Urpi, A., González-Torres, M., Abian, J., ... & Quintana, A. (2020). Mitochondrial proteome of affected glutamatergic neurons in a mouse model of Leigh syndrome. Frontiers in Cell and Developmental Biology, 8, 660.

Mouse Anti-ATP5IF1 Recombinant Antibody (A4062) in IF

BTSC73 and BTSC147 were subjected to immunostaining using antibodies to OSMR (green) and the mitochondrial matrix protein ATP synthase inhibitor F1 (ATPIF1, red). Nuclei were stained with DAPI. White rectangles mark the inset to demonstrate the co-localization of OSMR with ATPIF1. ...View More

Sharanek, A., Burban, A., Laaper, M., Heckel, E., Joyal, J. S., Soleimani, V. D., & Jahani-Asl, A. (2020). OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nature communications, 11(1), 4116.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A4062

Application

ICC, IF, WB, IHC

Immunogen

Recombinant IF1.

Specificity

Human, Cattle, Mouse, Rat, Dog

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
IF(ICC)	1-5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP Synthase Inhibitory Factor Subunit 1

Introduction

ATP5IF1 is a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

Entrez Gene ID

Human	93974
Mouse	11983
Rat	25392
Cattle	327699

UniProt ID

Human	Q9UII2
Mouse	O35143
Rat	Q03344
Cattle	P01096

Alternative Names

ATP Synthase Inhibitory Factor Subunit 1; ATPase Inhibitory Factor 1; ATPIF1; ATP Synthase Inhibitor Protein; Inhibitor Of F(1)F(O)-ATPase; ATPase Inhibitor Protein; IF(1);

Function

Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.

Biological Process

Angiogenesis Source: UniProtKB
Erythrocyte differentiation Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Heme biosynthetic process Source: UniProtKB
Mitochondrial depolarization Source: ParkinsonsUK-UCL
Negative regulation of ATPase activity Source: ParkinsonsUK-UCL
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of hydrolase activity Source: UniProtKB
Positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: Ensembl
Positive regulation of proteolysis involved in cellular protein catabolic process Source: ParkinsonsUK-UCL
Reactive oxygen species metabolic process Source: Ensembl
Regulation of ATP metabolic process Source: ParkinsonsUK-UCL
Regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL

Cellular Location

Mitochondrion

PTM

Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.

Esparza-Moltó, P. B., Nuevo-Taρioles, C., Chamorro, M., Nájera, L., Torresano, L., Santacatterina, F., & Cuezva, J. M. (2019). Tissue‐specific expression and post‐transcriptional regulation of the ATPase inhibitory factor 1 (IF1) in human and mouse tissues. The FASEB Journal, 33(2), 1836-1851.

García-Aguilar, A., & Cuezva, J. M. (2018). A review of the inhibition of the mitochondrial ATP synthase by IF1 in vivo: reprogramming energy metabolism and inducing mitohormesis. Frontiers in physiology, 9, 1322.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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