Mouse Anti-ATP6AP1 Recombinant Antibody (85) (CBMAB-A0634-LY)

Name: Mouse Anti-ATP6AP1 Recombinant Antibody (85)
SKU: CBMAB-A0634-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATP6AP1 Recombinant Antibody (85) in Immunoblot

Representative immunoblots of IP of WT or L37F NSP6 and ATP6AP1. ...View More

Sun, X., Liu, Y., Huang, Z., Xu, W., Hu, W., Yi, L., ... & Wu, W. K. K. (2022). SARS-CoV-2 non-structural protein 6 triggers NLRP3-dependent pyroptosis by targeting ATP6AP1. Cell Death & Differentiation, 29(6), 1240-1254.

Mouse Anti-ATP6AP1 Recombinant Antibody (85) in Immunoblot

Representative immunoblots with anti-ATP6AP1, ATP6V1A and b-actin using cortical lysates from WTand Ppt1/mice. ...View More

Perez-Canamas, A., Takahashi, H., Lindborg, J. A., & Strittmatter, S. M. (2021). Fronto-temporal dementia risk gene TMEM106B has opposing effects in different lysosomal storage disorders. Brain Communications, 3(1), fcaa200.

Mouse Anti-ATP6AP1 Recombinant Antibody (85) in WB

Representative western blot analysis of EEA1, Rab13, and LAMP1 protein levels in immortalized Schwann cells and HEK293 cells with stable knockdown of ATP6AP1, ATP6AP2, or control. Tubulin was used as protein loading control. Quantification (right) of EEA1, Rab13, and LAMP1 protein levels relative to control. Experiments were performed in replicates (Schwann cells, n ≥ 5; HEK293 cells, n ≥ 4). Error bars, mean ± S.D.; n.s., non significant; *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed unpaired t-test. ...View More

Pareja, F., Brandes, A. H., Basili, T., Selenica, P., Geyer, F. C., Fan, D., ... & Reis-Filho, J. S. (2018). Loss-of-function mutations in ATP6AP1 and ATP6AP2 in granular cell tumors. Nature Communications, 9(1), 3533.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, IP, ELISA

Immunogen

Recombinant ATP6AP1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATPase H+ Transporting Accessory Protein 1

Introduction

This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq]

Entrez Gene ID

Human	537
Rat	83615

UniProt ID

Human	Q15904
Rat	O54715

Alternative Names

16A; ATP6IP1; ATP6S1; Ac45; CF2; MGC129781; VATPS1; XAP-3; XAP3

Function

Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca2+-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).

Biological Process

Cellular iron ion homeostasis Source: UniProtKB
Cellular response to increased oxygen levels Source: UniProtKB
Establishment of organelle localization Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
pH reduction Source: UniProtKB
Positive regulation of bone resorption Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of exocytosis Source: UniProtKB
Positive regulation of osteoblast differentiation Source: UniProtKB
Positive regulation of osteoclast development Source: UniProtKB
Proton transmembrane transport Source: ProtInc
Regulation of cellular pH Source: GO_Central
Transferrin transport Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Not detected in trans-Golgi network.

Involvement in disease

Immunodeficiency 47 (IMD47): A complex immunodeficiency syndrome characterized by hypogammaglobulinemia, recurrent bacterial infections, defective glycosylation of serum proteins, and liver disease with neonatal jaundice and hepatosplenomegaly. Some patients may also have neurologic features, including seizures, mild intellectual disability, and behavioral abnormalities. Inheritance is X-linked recessive.

Topology

Vacuolar: 42-419 aa
Helical: 420-440 aa
Cytoplasmic: 441-470 aa

PTM

N-glycosylated.

Wang, J., Liu, Y., & Zhang, S. (2021). Prognostic and immunological value of ATP6AP1 in breast cancer: implications for SARS-CoV-2. Aging (Albany NY), 13(13), 16904.

Tvina, A., Thomsen, A., & Palatnik, A. (2020). Prenatal and postnatal phenotype of a pathologic variant in the ATP6AP1 gene. European journal of medical genetics, 63(6), 103881.

Ondruskova, N., Honzik, T., Vondrackova, A., Stranecky, V., Tesarova, M., Zeman, J., & Hansikova, H. (2020). Severe phenotype of ATP6AP1‐CDG in two siblings with a novel mutation leading to a differential tissue‐specific ATP6AP1 protein pattern, cellular oxidative stress and hepatic copper accumulation. Journal of inherited metabolic disease, 43(4), 694-700.

Dimitrov, B., Himmelreich, N., Ederveen, A. L. H., Lüchtenborg, C., Okun, J. G., Breuer, M., ... & Thiel, C. (2018). Cutis laxa, exocrine pancreatic insufficiency and altered cellular metabolomics as additional symptoms in a new patient with ATP6AP1-CDG. Molecular genetics and metabolism, 123(3), 364-374.

Dimitrov, B. (2017). Klinische, molekularbiologische und biochemische Charakterisierung eines neuen Patientendefekts im „ATPase H+-transporting accessory protein 1 “(ATP6AP1)-Gen (Doctoral dissertation).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ATP6AP1 Matched Antibody Pair (117) (CAT#: APMAB-117LY)

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Mouse Anti-ATP6AP1 Recombinant Antibody (CBT4152) (CAT#: V2LY-0625-LY1742)

ATP6AP1 Matched Antibody Pair (117) (CAT#: APMAB-117LY)

Isotype control

For research use only. Not intended for any clinical use.

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