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Mouse Anti-ATP6AP1 Recombinant Antibody (85) (CBMAB-A0634-LY)

The product is antibody recognizes ATP6AP1. The antibody 3A2 immunoassay techniques such as: WB, ELISA.
See all ATP6AP1 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
85
Antibody Isotype
IgG1, κ
Application
WB, IP, ELISA

Basic Information

Immunogen
Recombinant ATP6AP1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATPase H+ Transporting Accessory Protein 1
Introduction
This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq]
Entrez Gene ID
Human537
Rat83615
UniProt ID
HumanQ15904
RatO54715
Alternative Names
16A; ATP6IP1; ATP6S1; Ac45; CF2; MGC129781; VATPS1; XAP-3; XAP3
Function
Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca2+-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
Biological Process
Cellular iron ion homeostasis Source: UniProtKB
Cellular response to increased oxygen levels Source: UniProtKB
Establishment of organelle localization Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
pH reduction Source: UniProtKB
Positive regulation of bone resorption Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of exocytosis Source: UniProtKB
Positive regulation of osteoblast differentiation Source: UniProtKB
Positive regulation of osteoclast development Source: UniProtKB
Proton transmembrane transport Source: ProtInc
Regulation of cellular pH Source: GO_Central
Transferrin transport Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Not detected in trans-Golgi network.
Involvement in disease
Immunodeficiency 47 (IMD47): A complex immunodeficiency syndrome characterized by hypogammaglobulinemia, recurrent bacterial infections, defective glycosylation of serum proteins, and liver disease with neonatal jaundice and hepatosplenomegaly. Some patients may also have neurologic features, including seizures, mild intellectual disability, and behavioral abnormalities. Inheritance is X-linked recessive.
Topology
Vacuolar: 42-419 aa
Helical: 420-440 aa
Cytoplasmic: 441-470 aa
PTM
N-glycosylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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