Mouse Anti-ATP6V1A Recombinant Antibody (4F5) (CBMAB-A4083-YC)

Name: Mouse Anti-ATP6V1A Recombinant Antibody (4F5)
SKU: CBMAB-A4083-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4F5

Application

ELISA, IF, IHC-P, WB

Immunogen

ATP6V1A (NP_001681, 508-617 aa) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 508-617

More Infomation

Target

Full Name

ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A

Introduction

ATP6V1A is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zy

Entrez Gene ID

523

UniProt ID

P38606

Alternative Names

ATPase H+ Transporting V1 Subunit A; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; Vacuolar Proton Pump Subunit Alpha; Vacuolar ATPase Isoform VA68; V-ATPase Subunit A; EC 3.6.3.14; ATP6V1A1; ATP6A1; VPP2; ATPase, H+ Transporting, Lysosomal (Vac

Function

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
May play a role in neurite development and synaptic connectivity (PubMed:29668857).
(Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.

Biological Process

Cellular iron ion homeostasis Source: UniProtKB
Cellular response to amino acid starvation Source: Reactome
Cellular response to increased oxygen levels Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: GO_Central
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome

Cellular Location

Cytoplasm; Secretory vesicle. Co-localizes with WFS1 in the secretory granules in neuroblastoma cell lines.

Involvement in disease

Cutis laxa, autosomal recessive, 2D (ARCL2D): A form of cutis laxa, a disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, and a general connective tissue weakness. Most ARCL2D patients exhibit severe hypotonia as well as cardiovascular and neurologic involvement.
Epileptic encephalopathy, infantile or early childhood, 3 (IECEE3): A form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. IECEE3 is an autosomal dominant form characterized by onset of seizures in the first years of life.The severity of the phenotype is highly variable: some patients may be non-verbal and non-ambulatory with spastic quadriparesis and poor eye contact, whereas others have moderate intellectual disability.

PTM

Phosphorylation at Ser-384 by AMPK downregulates its enzyme activity.

Zhou, Z., Bai, J., Zhong, S., Zhang, R., Kang, K., Zhang, X., ... & Zhao, M. (2021). Downregulation of ATP6V1A Involved in Alzheimer’s Disease via Synaptic Vesicle Cycle, Phagosome, and Oxidative Phosphorylation. Oxidative medicine and cellular longevity, 2021.

Ip, Y. K., Hiong, K. C., Lim, L. J., Choo, C. Y., Boo, M. V., Wong, W. P., ... & Chew, S. F. (2018). Molecular characterization, light-dependent expression, and cellular localization of a host vacuolar-type H+-ATPase (VHA) subunit A in the giant clam, Tridacna squamosa, indicate the involvement of the host VHA in the uptake of inorganic carbon and its supply to the symbiotic zooxanthellae. Gene, 659, 137-148.

Fassio, A., Esposito, A., Kato, M., Saitsu, H., Mei, D., Marini, C., ... & Guerrini, R. (2018). De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy. Brain, 141(6), 1703-1718.

Wang, P., Wang, L., Sha, J., Lou, G., Lu, N., Hang, B., ... & Zou, X. (2017). Expression and transcriptional regulation of human ATP6V1A gene in gastric cancers. Scientific reports, 7(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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