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Mouse Anti-ATP6V1A Recombinant Antibody (CBYC-A884) (CBMAB-A4085-YC)

Provided herein is a Mouse monoclonal antibody against Human ATPase H+ Transporting V1 Subunit A. The antibody can be used for immunoassay techniques, such as IHC-P, WB.
See all ATP6V1A antibodies
Published Data
Mouse Anti-ATP6V1A Recombinant Antibody (CBYC-A884) in IP
Anti-GFP immunoprecipitation for the interaction between cystinosin-GFP and the Ragulator-Rag components. EV: empty vector. ...View More
Guo, X., Schmiege, P., Assafa, T. E., Wang, R., Xu, Y., Donnelly, L., ... & Li, X. (2022). Structure and mechanism of human cystine exporter cystinosin. Cell, 185(20), 3739-3752.
Mouse Anti-ATP6V1A Recombinant Antibody (CBYC-A884) in Immunoblot
The assembly of V-ATPase V1-V0 holoenzymes after treatment with an α-tubulin deacetylase inhibitor or a microtubule depolymerizing agent. RPE1 cells were treated with VEH, tubacin (2.5 μM, 20 h), Noco (10 μg/ml, 6 h), or in combination. Cytosolic and membrane fractions were then extracted and analyzed by immunoblotting using antibodies against V-ATPase subunit V1A (ATP6V1A), V1C1 (ATP6V1C1), or V0D (ATP6V0D). LAMP1 and GAPDH were used as loading controls for membrane proteins and cytosolic proteins, respectively. ...View More
Tang, Q., Liu, M., Liu, Y., Hwang, R. D., Zhang, T., & Wang, J. (2021). NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification. The EMBO Journal, 40(19), e107204.
Mouse Anti-ATP6V1A Recombinant Antibody (CBYC-A884) in IF
Co-labelling of TBC1D25 and proteins expressed at the ruffled membrane. Human osteoclasts cultured on bone slices were double-stained using antibodies directed against TBC1D25 (red) and antibodies against Rab7 (2-A), α1 ATPase (2-B), and cathepsin K (2-C) (shown in green for clear comparison), and counterstaining was performed with DAPI (blue). Images are representative of 3 independent experiments. Optical sections at bone matrix level are shown (scale bar 10 μm). 2-C: Co-labelling of TBC1D25 and cathepsin K: Optical sections at bone matrix level are shown. Colocalization is represented using white for overlapped pixels. The signals were quantified and the overlap coefficient at the ruffled membrane is indicated. 2-D, 2E: Negative controls (mouse and rabbit Ig) are represented. Optical sections at nuclear level and bone matrix level are shown (scale bar 10 μm). ...View More
Roy, M., Stephens, E., Bouhour, S., & Roux, S. (2021). RabGAP TBC1D25 is involved in human osteoclast activity. European Journal of Cell Biology, 100(3), 151145.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A884
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IHC-P, WB, IP, FC

Basic Information

Immunogen
Amino acids 508-617 of V-ATPase α1 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Introduction
ATP6V1A is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zy
Entrez Gene ID
Human523
Mouse11964
Rat685232
UniProt ID
HumanP38606
MouseP50516
RatD4A133
Alternative Names
ATPase H+ Transporting V1 Subunit A; ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A; Vacuolar Proton Pump Subunit Alpha; Vacuolar ATPase Isoform VA68; V-ATPase Subunit A; EC 3.6.3.14; ATP6V1A1; ATP6A1; VPP2; ATPase, H+ Transporting, Lysosomal (Vac
Function
Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
May play a role in neurite development and synaptic connectivity (PubMed:29668857).
(Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.
Biological Process
Cellular iron ion homeostasis Source: UniProtKB
Cellular response to amino acid starvation Source: Reactome
Cellular response to increased oxygen levels Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: GO_Central
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Cellular Location
Cytoplasm; Secretory vesicle. Co-localizes with WFS1 in the secretory granules in neuroblastoma cell lines.
Involvement in disease
Cutis laxa, autosomal recessive, 2D (ARCL2D): A form of cutis laxa, a disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, and a general connective tissue weakness. Most ARCL2D patients exhibit severe hypotonia as well as cardiovascular and neurologic involvement.
Epileptic encephalopathy, infantile or early childhood, 3 (IECEE3): A form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. IECEE3 is an autosomal dominant form characterized by onset of seizures in the first years of life.The severity of the phenotype is highly variable: some patients may be non-verbal and non-ambulatory with spastic quadriparesis and poor eye contact, whereas others have moderate intellectual disability.
PTM
Phosphorylation at Ser-384 by AMPK downregulates its enzyme activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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