Mouse Anti-ATP6V1F Recombinant Antibody (1B8) (CBMAB-V0023-YC)

Name: Mouse Anti-ATP6V1F Recombinant Antibody (1B8)
SKU: CBMAB-V0023-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1B8

Application

WB, FC, IHC, ICC, IF

Immunogen

Full length human recombinant protein of human ATP6V1F produced in E.coli

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IF(ICC)	1:100
IHC	1:150
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATPase H+ Transporting V1 Subunit F

Introduction

ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. ATP6V1F is the V1 domain F subunit protein. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.

Entrez Gene ID

9296

UniProt ID

Q16864

Alternative Names

ATPase H+ Transporting V1 Subunit F; ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F; Vacuolar Proton Pump Subunit F; V-ATPase 14 KDa Subunit; V-ATPase Subunit F; ATP6S14; VATF; H(+)-Transporting Two-Sector ATPase, 14kD Subunit

Function

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Biological Process

Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Transferrin transport Source: Reactome

Cellular Location

Cytosol; Extracellular exosome; Membrane; Proton-transporting two-sector ATPase complex; Proton-transporting V-type ATPase, V1 domain; Vacuolar proton-transporting V-type ATPase complex

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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