Human Recombinant ATP6V1F protein, GST Tag (V2LY-0526-LY2204)

Name: Human Recombinant ATP6V1F protein, GST Tag
SKU: V2LY-0526-LY2204
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ATP6V1F protein, GST Tag consist of Amino Acid: 1-119 and predicts a molecular mass of 40.2 kDa.

Molecule Mass

40.2 kDa

Sequence

Amino Acid: 1-119

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ATPase H+ Transporting V1 Subunit F

Function

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Biological Process

Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Transferrin transport Source: Reactome

Cellular Location

Cytosol; Extracellular exosome; Membrane; Proton-transporting two-sector ATPase complex; Proton-transporting V-type ATPase, V1 domain; Vacuolar proton-transporting V-type ATPase complex

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ATP6V1F protein, Biotin Conjugated, GST Tag (CAT#: V2LY-0526-LY2205)

Related Products

Mouse Anti-ATP6V1F Recombinant Antibody (1B8) (CAT#: CBMAB-V0023-YC)

Human Recombinant ATP6V1F protein, Biotin Conjugated, GST Tag (CAT#: V2LY-0526-LY2205)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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