Mouse Anti-ATP9A Recombinant Antibody (3G2) (CBMAB-A0653-LY)

The product is antibody recognizes ATP9A. The antibody 3G2 immunoassay techniques such as: WB, ELISA.
See all ATP9A antibodies

Published Data

Fig. 1 Co-immunoprecipitation of csecretase components with ATP9A and Trk-B.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3G2

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

ATP9A (NP_006036, 358 a.a. ~ 456 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ATPase, class II, type 9A

Entrez Gene ID

10079

UniProt ID

O75110

Alternative Names

ATPIIA; KIAA0611

Function

Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport (PubMed:27733620, PubMed:30213940).
In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation (PubMed:30213940).
Appears to be implicated in intercellular communication by negatively regulating the release of exosomes (PubMed:30947313).
The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved (PubMed:30947313).

Biological Process

Endocytosis Source: GO_Central
Negative regulation of exosomal secretion Source: UniProtKB
Phospholipid translocation Source: GO_Central
Regulation of endocytic recycling Source: UniProtKB
Regulation of retrograde transport, endosome to Golgi Source: UniProtKB
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central

Cellular Location

Early endosome membrane; Recycling endosome membrane; Cell membrane; Trans-Golgi network membrane. Efficient exit from the endoplasmic reticulum does not require TMEM30A, nor TMEM30B (PubMed:21914794). Transiently expressed in the plasma membrane (PubMed:30947313).

Topology

Cytoplasmic: 2-69 aa
Helical: 70-91 aa
Extracellular: 92-96 aa
Helical: 97-119 aa
Cytoplasmic: 120-303 aa
Helical: 304-325 aa
Extracellular: 326-332 aa
Helical: 333-354 aa
Cytoplasmic: 355-841 aa
Helical: 842-862 aa
Extracellular: 863-874 aa
Helical: 875-893 aa
Cytoplasmic: 894-923 aa
Helical: 924-942 aa
Extracellular: 943-949 aa
Helical: 950-972 aa
Cytoplasmic: 973-978 aa
Helical: 979-999 aa
Extracellular: 1000-1006 aa
Helical: 1007-1030 aa
Cytoplasmic: 1031-1047 aa

References

Vogt, G., Verheyen, S., Schwartzmann, S., Ehmke, N., Potratz, C., Schwerin-Nagel, A., ... & Boschann, F. (2021). Biallelic truncating variants in ATP9A cause a novel neurodevelopmental disorder involving postnatal microcephaly and failure to thrive. Journal of Medical Genetics.

Mattioli, F., Darvish, H., Paracha, S. A., Tafakhori, A., Firouzabadi, S. G., Chapi, M., ... & Ansar, M. (2021). Biallelic truncation variants in ATP9A are associated with a novel autosomal recessive neurodevelopmental disorder. medRxiv.

Xu, X., Xu, L., Zhang, P., Ouyang, K., Xiao, Y., Xiong, J., ... & Duan, L. (2020). Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition. Oxidative Medicine and Cellular Longevity, 2020.

Naik, J., Hau, C. M., Ten Bloemendaal, L., Mok, K. S., Hajji, N., Wehman, A. M., ... & Bosma, P. J. (2019). The P4-ATPase ATP9A is a novel determinant of exosome release. PloS one, 14(4), e0213069.

McGough, I. J., De Groot, R. E., Jellett, A. P., Betist, M. C., Varandas, K. C., Danson, C. M., ... & Cullen, P. J. (2018). SNX3-retromer requires an evolutionary conserved MON2: DOPEY2: ATP9A complex to mediate Wntless sorting and Wnt secretion. Nature communications, 9(1), 1-13.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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