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Rabbit Anti-AUP1 Recombinant Antibody (D5M9Q) (CBMAB-A4174-YC)

Provided herein is a Rabbit monoclonal antibody against Human AUP1, Lipid Droplet Regulating VLDL Assembly Factor. The antibody can be used for immunoassay techniques, such as IP, WB.
See all AUP1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D5M9Q
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gln320 of human AUP1, long isoform.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AUP1, Lipid Droplet Regulating VLDL Assembly Factor
Introduction
AUP1 is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigy
Entrez Gene ID
Human550
Mouse11993
Rat680423
UniProt ID
HumanQ9Y679
MouseP70295
RatA1L134
Alternative Names
AUP1, Lipid Droplet Regulating VLDL Assembly Factor; Ancient Ubiquitous Protein 1;
Function
Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome (PubMed:18711132, PubMed:21857022).
Plays a role in lipid droplet formation (PubMed:21857022).
Induces lipid droplet clustering (PubMed:24039768).
Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation (PubMed:23223569, PubMed:21127063).
Also required for the degradation of INSIG1, SREBF1 and SREBF2 (PubMed:23223569).
Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB (PubMed:28183703).
(Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles.
Biological Process
ER-associated misfolded protein catabolic process Source: UniProtKB
Lipid droplet formation Source: UniProtKB
Lipid droplet organization Source: UniProtKB
Lipophagy Source: UniProtKB
Protein localization to lipid droplet Source: UniProtKB
Response to virus Source: UniProtKB
Retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Ubiquitin-dependent ERAD pathway Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum membrane; Lipid droplet; Autophagosome. (Microbial infection) Upon Dengue virus infection, relocates from lipid droplets to autophagosomes.
Topology
Cytoplasmic: 1-20 aa
Intramembrane: 21-41 aa
Cytoplasmic: 42-410 aa
PTM
Monoubiquitinated and diubiquitinated.
(Microbial infection) Not ubiquitinated following Dengue virus infection.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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