Mouse Anti-AXIN1 Recombinant Antibody (2B11) (CBMAB-A4221-YC)

Name: Mouse Anti-AXIN1 Recombinant Antibody (2B11)
SKU: CBMAB-A4221-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AXIN1 Recombinant Antibody (2B11) in Immunoblot

Phosphorylation of Axin and its total component have been reduced in RV infected cells. Expression of Nkd1 is suppressed in RV infected cells. ...View More

Banerjee, A., Chawla-Sarkar, M., & Mukherjee, A. (2022). Rotavirus-Mediated Suppression of miRNA-192 Family and miRNA-181a Activates Wnt/β-Catenin Signaling Pathway: An In Vitro Study. Viruses, 14(3), 558.

Mouse Anti-AXIN1 Recombinant Antibody (2B11) in WB

Western blot analysis of the protein expression of Wnt/b-catenin signaling-related genes, including GSK-3b, Axin, Dvl-2, bcatenin, TCF4, LEF1 and c-Myc in control or ASPM-KD SMMC7721 and HepG2 cells. ...View More

Zhang, H., Yang, X., Zhu, L., Li, Z., Zuo, P., Wang, P., ... & Ye, H. (2021). ASPM promotes hepatocellular carcinoma progression by activating Wnt/β-catenin signaling through antagonizing autophagy-mediated Dvl2 degradation. FEBS Open bio, 11(10), 2784-2799.

Mouse Anti-AXIN1 Recombinant Antibody (2B11) in IP

Coimmunoprecipitation of β-catenin, Shc3, Axin, and GSK-3β, which were pulled down using anti-β-catenin or anti-Shc3 in MHCC97L cells. ...View More

Liu, Y., Zhuang, H., Cao, F., Li, J., Guo, Y., Zhang, J., ... & Liu, Y. (2021). Shc3 promotes hepatocellular carcinoma stemness and drug resistance by interacting with β-catenin to inhibit its ubiquitin degradation pathway. Cell Death & Disease, 12(3), 278.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2B11

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

AXIN1 AA 643-740 partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Axin 1

Introduction

AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protei

Entrez Gene ID

8312

UniProt ID

O15169

Alternative Names

Axin 1; Protein Phosphatase 1, Regulatory Subunit 49; Axis Inhibition Protein 1; AXIN; Fused, Mouse, Homolog Of; Axis Inhibitor 1; PPP1R49; Axin-1; HAxin;

Function

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453).
Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039).
In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039).
Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693).
Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684).
Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684).

Biological Process

Activation of protein kinase activity Source: BHF-UCL
Apoptotic process Source: UniProtKB-KW
Beta-catenin destruction complex assembly Source: Reactome
Beta-catenin destruction complex disassembly Source: Reactome
Cellular protein-containing complex assembly Source: BHF-UCL
Multicellular organism development Source: UniProtKB-KW
Negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Positive regulation of canonical Wnt signaling pathway Source: Reactome
Positive regulation of JNK cascade Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Positive regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
Positive regulation of protein catabolic process Source: BHF-UCL
Positive regulation of protein phosphorylation Source: BHF-UCL
Positive regulation of protein ubiquitination Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of ubiquitin-dependent protein catabolic process Source: BHF-UCL
Positive regulation of ubiquitin-protein transferase activity Source: BHF-UCL
Regulation of intracellular estrogen receptor signaling pathway Source: Reactome
Regulation of Wnt signaling pathway Source: Reactome
Wnt signaling pathway Source: Reactome

Cellular Location

Cell membrane; Cytoplasm; Nucleus; Membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).

Involvement in disease

Hepatocellular carcinoma (HCC): A primary malignant neoplasm of epithelial liver cells. The major risk factors for HCC are chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, prolonged dietary aflatoxin exposure, alcoholic cirrhosis, and cirrhosis due to other causes.
Caudal duplication anomaly (CADUA): A condition characterized by the occurrence of duplications of different organs in the caudal region.

PTM

Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

Wang, W., Liu, P., Lavrijsen, M., Li, S., Zhang, R., Li, S., ... & Smits, R. (2021). Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines. Scientific reports, 11(1), 1-13.

Li, J., Knudsen, J. R., Henriquez‐Olguin, C., Li, Z., Birk, J. B., Persson, K. W., ... & Jensen, T. E. (2021). AXIN1 knockout does not alter AMPK/mTORC1 regulation and glucose metabolism in mouse skeletal muscle. The Journal of Physiology, 599(12), 3081-3100.

Yang, W., Cui, G., Ding, M., Yang, M., & Dai, D. (2020). MicroRNA‐124‐3p. 1 promotes cell proliferation through Axin1‐dependent Wnt signaling pathway and predicts a poor prognosis of triple‐negative breast cancer. Journal of clinical laboratory analysis, 34(7), e23266.

Said, N. M., Moustafa, H. Y., & Abdel Aziz, S. (2020). Association between AXIN1 gene polymorphisms (wnt signaling pathway gene) and nephropathy induced by diabetes and hypertension in the Egyptian population. Biochemistry Letters, 16(1), 128-150.

Garrett, S., Zhang, Y. G., & Sun, J. (2019). Intestinal Axin1 Regulation of Epithelial Functions and Host‐Microbial Interactions in Health and Inflammation. The FASEB Journal, 33(S1), 764-5.

Ek, M., Roth, B., Engström, G., & Ohlsson, B. (2019). AXIN1 in plasma or serum is a potential new biomarker for endometriosis. International journal of molecular sciences, 20(1), 189.

Qiao, Y., Wang, J., Karagoz, E., Liang, B., Song, X., Shang, R., ... & Chen, X. (2019). Axis inhibition protein 1 (Axin1) deletion–induced hepatocarcinogenesis requires intact β‐catenin but not notch cascade in mice. Hepatology, 70(6), 2003-2017.

Khan, I., Ansari, I. A., Singh, P., Dass, J. F. P., & Khan, F. (2018). Identification and characterization of functional single nucleotide polymorphisms (SNPs) in Axin 1 gene: a molecular dynamics approach. Cell biochemistry and biophysics, 76(1), 173-185.

Abitbol, S., Dahmani, R., Coulouarn, C., Ragazzon, B., Mlecnik, B., Senni, N., ... & Gilgenkrantz, H. (2018). AXIN deficiency in human and mouse hepatocytes induces hepatocellular carcinoma in the absence of β-catenin activation. Journal of hepatology, 68(6), 1203-1213.

Picco, G., Petti, C., Centonze, A., Torchiaro, E., Crisafulli, G., Novara, L., ... & Medico, E. (2017). Loss of AXIN1 drives acquired resistance to WNT pathway blockade in colorectal cancer cells carrying RSPO 3 fusions. EMBO molecular medicine, 9(3), 293-303.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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