Mouse Anti-AZIN1 Recombinant Antibody (1F11) (CBMAB-A0676-LY)

Name: Mouse Anti-AZIN1 Recombinant Antibody (1F11)
SKU: CBMAB-A0676-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F11

Application

WB, ELISA

Immunogen

AZIN1 (NP_056962, 339 a.a. ~ 447 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

antizyme inhibitor 1

Introduction

Ornithine decarboxylase (ODC) catalyzes the conversion of ornithine to putrescine in the first and apparently rate-limiting step in polyamine biosynthesis. Ornithine decarboxylase antizymes play a role in the regulation of polyamine synthesis by binding to and inhibiting ornithine decarboxylase. The protein encoded by this gene is highly similar to ODC. It binds to ODC antizyme and stabilizes ODC, thus inhibiting antizyme-mediated ODC degradation. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Entrez Gene ID

51582

UniProt ID

O14977

Alternative Names

MGC3832; MGC691; OAZI; OAZIN; ODC1L

Function

Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding (PubMed:17900240, PubMed:26305948).
Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production (PubMed:17900240).

Biological Process

Negative regulation of protein catabolic process Source: UniProtKB
Positive regulation of polyamine transmembrane transport Source: UniProtKB
Putrescine biosynthetic process from ornithine Source: GO_Central
Regulation of cellular amino acid metabolic process Source: Reactome

Cellular Location

Nucleus

PTM

Ubiquitinated, leading to its proteasomal degradation; a process that is reduced in presence of antizyme OAZ1.

Tay, D. J. T., Song, Y., Peng, B., Toh, T. B., Hooi, L., Toh, D. F. K., ... & Chen, L. (2021). Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer. Molecular Therapy.

Chen, C. H., Wang, Y. H., Tsai, S. F., Yu, T. M., Chen, S. Y., & Tsai, F. J. (2019). Antizyme inhibitor 1 genetic polymorphisms associated with diabetic patients validated in the livers of diabetic mice. Experimental and therapeutic medicine, 18(4), 3139-3146.

Wang, F., He, J., Cheng, H., Ma, Y., Liu, S., Yang, L., ... & Cheng, T. (2019). RNA EDITOME ANALYSIS OF HEMATOPOIETIC CELLS REVEALS ANTIZYME INHIBITOR 1 AS A FUNCTIONAL REGULATOR IN HEMATOPOIETIC STEM AND PROGENITOR CELLS. Experimental Hematology, 76, S91.

Hu, X., Chen, J., Shi, X., Feng, F., Lau, K. W., Chen, Y., ... & Li, J. (2017). RNA editing of AZIN1 induces the malignant progression of non-small-cell lung cancers. Tumor Biology, 39(8), 1010428317700001.

Qiu, S., Liu, J., & Xing, F. (2017). Antizyme inhibitor 1: a potential carcinogenic molecule. Cancer science, 108(2), 163-169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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