Rat Anti-B3GALT5 Recombinant Antibody (CBYY-0071) (CBMAB-0071-YY)

This product is rat antibody that recognizes B3GALT5. The antibody CBYY-0071 can be used for immunoassay techniques such as: WB, FC, IF, IHC, IHC-Fr, IHC-P, IP
See all B3GALT5 antibodies

Datasheet

Summary

Host Animal

Rat

Specificity

Human, Mouse

Clone

CBYY-0071

Antibody Isotype

IgM

Application

WB, FC, IF, IHC, IP

Basic Information

Immunogen

4-8 cell stage mouse embryos.

Specificity

Human, Mouse

Antibody Isotype

IgM

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Beta-1,3-Galactosyltransferase 5

Introduction

This gene encodes a member of a family of membrane-bound glycoproteins. The encoded protein may synthesize type 1 Lewis antigens, which are elevated in gastrointestinal and pancreatic cancers. Alternatively spliced transcript variants using multiple alternate promoters have been observed for this gene. [provided by RefSeq, Sep 2017]

Entrez Gene ID

Human	10317
Mouse	93961

UniProt ID

Human	Q9Y2C3
Mouse	Q9JI67

Alternative Names

Beta-1,3-Galactosyltransferase 5; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,3-Galactosyltransferase 5; UDP-Gal:BetaGlcNAc Beta 1,3-Galactosyltransferase, Polypeptide 5; Homolog Of C. Elegans Bt Toxin Resistance Gene Bre-5; Beta-1,3-GalTase 5; Beta-3-Gx-T5; Beta3Gal-T5; UDP-Gal:Beta-GlcNAc Beta-1,3-Galactosyltransferase 5; UDP-Gal:BetaGlcNAc Beta 1,3-Galactosyltransferase 5; GlcNAc-Beta-1,3-Galactosyltransferase 5;

Function

Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.

Biological Process

Oligosaccharide biosynthetic process Source: Reactome
Poly-N-acetyllactosamine biosynthetic process Source: GO_Central
Protein glycosylation Source: GO_Central

Cellular Location

Golgi apparatus membrane

Topology

Cytoplasmic: 1-7 aa
Helical: 8-28 aa
Lumenal: 29-310 aa

References

Liao, Y. M., Wang, Y. H., Hung, J. T., Lin, Y. J., Huang, Y. L., Liao, G. S., ... & Alice, L. Y. (2021). High B3GALT5 expression confers poor clinical outcome and contributes to tumor progression and metastasis in breast cancer. Breast Cancer Research, 23(1), 1-13.

Lin, R. J., Kuo, M. W., Yang, B. C., Tsai, H. H., Chen, K., Huang, J. R., ... & Yu, J. (2020). B3GALT5 knockout alters glycosphingolipid profile and facilitates transition to human naïve pluripotency. Proceedings of the National Academy of Sciences, 117(44), 27435-27444.

Cai, B. H., Lee, H. Y., Chou, C. K., Wu, P. H., Huang, H. C., Chao, C. C., ... & Kannagi, R. (2020). SSEA3 and Sialyl Lewis a glycan expression is controlled by B3GALT5 LTR through Lamin A-NFYA and SIRT1-STAT3 signaling in human ES cells. Cells, 9(1), 177.

Kuo, H. H., Lin, R. J., Hung, J. T., Hsieh, C. B., Hung, T. H., Lo, F. Y., ... & Alice, L. Y. (2017). High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma. Scientific reports, 7(1), 1-13.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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