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Mouse Anti-B3GAT3 Recombinant Antibody (CBYY-0080) (CBMAB-0080-YY)

This product is mouse antibody that recognizes B3GAT3. The antibody CBYY-0080 can be used for immunoassay techniques such as: ELISA, WB
See all B3GAT3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0080
Antibody Isotype
IgM, κ
Application
ELISA, WB, IHC-P, IF, IP

Basic Information

Immunogen
Amino acids 323-334 at the C-terminus of GlcAT-I of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
Introduction
The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans. A pseudogene of this gene has been identified on chromosome 3.
Entrez Gene ID
26229
UniProt ID
O94766
Alternative Names
Beta-1,3-Glucuronyltransferase 3; Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 3; Beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I); UDP-GlcUA:Gal Beta-1,3-Gal-R Glucuronyltransferase; Glucuronosyltransferase I; EC 2.4.1.135;
Function
Glycosaminoglycans biosynthesis (PubMed:25893793).
Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation (PubMed:24425863).
Biological Process
Carbohydrate metabolic process Source: GO_Central
Chondroitin sulfate proteoglycan biosynthetic process Source: MGI
Dermatan sulfate proteoglycan biosynthetic process Source: MGI
Glycosaminoglycan biosynthetic process Source: UniProtKB
Glycosaminoglycan metabolic process Source: Reactome
Heparan sulfate proteoglycan biosynthetic process Source: MGI
Positive regulation of catalytic activity Source: UniProtKB
Positive regulation of intracellular protein transport Source: UniProtKB
Protein glycosylation Source: UniProtKB-UniPathway
Cellular Location
Golgi apparatus membrane; Cis-Golgi network
Involvement in disease
Multiple joint dislocations, short stature, and craniofacial dysmorphism with or without congenital heart defects (JDSCD): An autosomal recessive disease characterized by dysmorphic facies, bilateral dislocations of the elbows, hips, and knees, clubfeet, and short stature, as well as cardiovascular defects.
Topology
Cytoplasmic: 1-7 aa
Helical: 8-28 aa
Lumenal: 29-335 aa
PTM
N-glycosylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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