Human Recombinant B3GAT3 protein, His Tag (V2LY-0526-LY2232)

Name: Human Recombinant B3GAT3 protein, His Tag
SKU: V2LY-0526-LY2232
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant B3GAT3 protein, His Tag consist of Amino Acid: 72-335 and predicts a molecular mass of 31.5 kDa.

Molecule Mass

31.5 kDa

Sequence

Amino Acid: 72-335

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)

Function

Glycosaminoglycans biosynthesis (PubMed:25893793).
Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation (PubMed:24425863).

Biological Process

Carbohydrate metabolic process Source: GO_Central
Chondroitin sulfate proteoglycan biosynthetic process Source: MGI
Dermatan sulfate proteoglycan biosynthetic process Source: MGI
Glycosaminoglycan biosynthetic process Source: UniProtKB
Glycosaminoglycan metabolic process Source: Reactome
Heparan sulfate proteoglycan biosynthetic process Source: MGI
Positive regulation of catalytic activity Source: UniProtKB
Positive regulation of intracellular protein transport Source: UniProtKB
Protein glycosylation Source: UniProtKB-UniPathway

Cellular Location

Golgi apparatus membrane; Cis-Golgi network

Involvement in disease

Multiple joint dislocations, short stature, and craniofacial dysmorphism with or without congenital heart defects (JDSCD): An autosomal recessive disease characterized by dysmorphic facies, bilateral dislocations of the elbows, hips, and knees, clubfeet, and short stature, as well as cardiovascular defects.

Topology

Cytoplasmic: 1-7 aa
Helical: 8-28 aa
Lumenal: 29-335 aa

PTM

N-glycosylated.

Colman, M., Van Damme, T., Steichen-Gersdorf, E., Laccone, F., Nampoothiri, S., Syx, D., ... & Malfait, F. (2019). The clinical and mutational spectrum of B3GAT3 linkeropathy: two case reports and literature review. Orphanet journal of rare diseases, 14(1), 1-10.

Yauy, K., Mau-Them, F. T., Willems, M., Coubes, C., Blanchet, P., Herlin, C., ... & Genevieve, D. (2018). B3GAT3-related disorder with craniosynostosis and bone fragility due to a unique mutation. Genetics in Medicine, 20(2), 269-274.

Bloor, S., Giri, D., Didi, M., & Senniappan, S. (2017). Novel splicing mutation in B3GAT3 associated with short stature, GH deficiency, Hypoglycaemia, developmental delay, and multiple congenital anomalies. Case reports in genetics, 2017.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-B3GAT3 Recombinant Antibody (CBYY-0080) (CAT#: CBMAB-0080-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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