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Rabbit Anti-BANF1 Recombinant Antibody (CBYY-0187) (CBMAB-0188-YY)

This product is rabbit antibody that recognizes BANF1. The antibody CBYY-0187 can be used for immunoassay techniques such as: WB, IHC-P, ICC
See all BANF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYY-0187
Antibody Isotype
IgG
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Synthetic peptide within Human BANF1 aa 1-50.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500
IF(ICC)1:50-1:100
IHC1:50-1:200
FC1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, pH 7.4, 40% glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
barrier to autointegration factor 1
Introduction
The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forMouse a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009]
Entrez Gene ID
Human8815
Mouse23825
Rat114087
Dog611961
UniProt ID
HumanO75531
MouseO54962
RatQ9R1T1
Alternative Names
Barrier To Autointegration Factor 1; Breakpoint Cluster Region Protein 1; BAF; Barrier-To-Autointegration Factor; D14S1460; BCRP1; BCRG1; NGPS;
Function
Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.
(Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
(Microbial infection) In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication.
Biological Process
Chromosome condensation Source: GO_Central
Chromosome segregation Source: GO_Central
DNA integration Source: Ensembl
Establishment of integrated proviral latency Source: Reactome
Mitotic nuclear envelope reassembly Source: Reactome
Negative regulation of viral genome replication Source: UniProtKB
Nuclear transport Source: Reactome
Response to virus Source: ProtInc
Cellular Location
Barrier-to-autointegration factor: Nucleus; Nucleus envelope; Cytoplasm; Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. The phosphorylated form (by VRK1 or vaccinia virus B1 kinase) shows a cytoplasmic localization whereas the unphosphorylated form locates almost exclusively in the nucleus (PubMed:24600006, PubMed:16495336). May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
Involvement in disease
Nestor-Guillermo progeria syndrome (NGPS): An atypical progeroid syndrome characterized by normal development in the first years of life, later followed by the emergence of generalized lipoatrophy, severe osteoporosis, and marked osteolysis. The atrophic facial subcutaneous fat pad and the marked osteolysis of the maxilla and mandible result in a typical pseudosenile facial appearance with micrognathia, prominent subcutaneous venous patterning, a convex nasal ridge, and proptosis. Cognitive development is completely normal. Patients do not have cardiovascular dysfunction, atherosclerosis, or metabolic anomalies.
PTM
Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2; Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to bind LEM domain-containing proteins. Non phosphorylated BAF seems to enhance binding between EMD and LMNA. Dephosphorylated by protein phosphatase 2A (PP2A) following interaction with ANKLE2/LEM4 during mitotic exit, leading to mitotic nuclear envelope reassembly.
(Microbial infection) Phosphorylated at the N-terminus by vaccinia virus (VacV) B1 kinase, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity (PubMed:24600006, PubMed:16495336). Hyperphosphorylation is linked to the loss of ability to suppress vaccinia virus replication (PubMed:24600006).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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