Mouse Anti-BANF1 Recombinant Antibody (CBYY-0189) (CBMAB-0190-YY)

Name: Mouse Anti-BANF1 Recombinant Antibody (CBYY-0189)
SKU: CBMAB-0190-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0189

Application

ELISA, WB

Immunogen

BANF1 (AAH05942, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

barrier to autointegration factor 1

Introduction

The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forMouse a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009]

Entrez Gene ID

8815

UniProt ID

O75531

Alternative Names

Barrier To Autointegration Factor 1; Breakpoint Cluster Region Protein 1; BAF; Barrier-To-Autointegration Factor; D14S1460; BCRP1; BCRG1; NGPS;

Function

Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.
(Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
(Microbial infection) In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication.

Biological Process

Chromosome condensation Source: GO_Central
Chromosome segregation Source: GO_Central
DNA integration Source: Ensembl
Establishment of integrated proviral latency Source: Reactome
Mitotic nuclear envelope reassembly Source: Reactome
Negative regulation of viral genome replication Source: UniProtKB
Nuclear transport Source: Reactome
Response to virus Source: ProtInc

Cellular Location

Barrier-to-autointegration factor: Nucleus; Nucleus envelope; Cytoplasm; Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. The phosphorylated form (by VRK1 or vaccinia virus B1 kinase) shows a cytoplasmic localization whereas the unphosphorylated form locates almost exclusively in the nucleus (PubMed:24600006, PubMed:16495336). May be included in HIV-1 virions via its interaction with viral GAG polyprotein.

Involvement in disease

Nestor-Guillermo progeria syndrome (NGPS): An atypical progeroid syndrome characterized by normal development in the first years of life, later followed by the emergence of generalized lipoatrophy, severe osteoporosis, and marked osteolysis. The atrophic facial subcutaneous fat pad and the marked osteolysis of the maxilla and mandible result in a typical pseudosenile facial appearance with micrognathia, prominent subcutaneous venous patterning, a convex nasal ridge, and proptosis. Cognitive development is completely normal. Patients do not have cardiovascular dysfunction, atherosclerosis, or metabolic anomalies.

PTM

Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2; Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to bind LEM domain-containing proteins. Non phosphorylated BAF seems to enhance binding between EMD and LMNA. Dephosphorylated by protein phosphatase 2A (PP2A) following interaction with ANKLE2/LEM4 during mitotic exit, leading to mitotic nuclear envelope reassembly.
(Microbial infection) Phosphorylated at the N-terminus by vaccinia virus (VacV) B1 kinase, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity (PubMed:24600006, PubMed:16495336). Hyperphosphorylation is linked to the loss of ability to suppress vaccinia virus replication (PubMed:24600006).

Burgess, J. T., Cheong, C. M., Suraweera, A., Sobanski, T., Beard, S., Dave, K., ... & Bolderson, E. (2021). Barrier-to-autointegration-factor (Banf1) modulates DNA double-strand break repair pathway choice via regulation of DNA-dependent kinase (DNA-PK) activity. Nucleic acids research, 49(6), 3294-3307.

Dharmaraj, T., Guan, Y., Liu, J., Badens, C., Gaborit, B., & Wilson, K. L. (2019). Rare BANF1 alleles and relatively frequent EMD alleles including ‘healthy lipid’emerin p. D149H in the ExAC cohort. Frontiers in cell and developmental biology, 7, 48.

Bolderson, E., Burgess, J. T., Li, J., Gandhi, N. S., Boucher, D., Croft, L. V., ... & Richard, D. J. (2019). Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage. Nature communications, 10(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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