Mouse Anti-BAP1 Recombinant Antibody (CBYY-0191) (CBMAB-0192-YY)

Name: Mouse Anti-BAP1 Recombinant Antibody (CBYY-0191)
SKU: CBMAB-0192-YY
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYY-0191

Application

ELISA, WB, IF, IP

Immunogen

Amino acids 430-729 of BAP1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BRCA1 Associated Protein 1

Introduction

This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]

Entrez Gene ID

8314

UniProt ID

Q92560

Alternative Names

BRCA1 Associated Protein 1; BRCA1 Associated Protein-1 (Ubiquitin Carboxy-Terminal Hydrolase); Cerebral Protein 6; Ubiquitin Carboxyl-Terminal Hydrolase BAP1; Ubiquitin Carboxy-Terminal Hydrolase; BRCA1-Associated Protein 1; Cerebral Protein-13;

Function

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922).
Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (PubMed:20436459, PubMed:25451922).
Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459).
Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555).
Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555).
Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993).
It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993).
Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950).
Acts as a tumor suppressor (PubMed:9528852).

Biological Process

Cellular protein modification process Source: UniProtKB
Double-strand break repair Source: Reactome
Macrophage homeostasis Source: Ensembl
Monoubiquitinated histone H2A deubiquitination Source: UniProtKB
Monoubiquitinated protein deubiquitination Source: UniProtKB
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein deubiquitination Source: UniProtKB
Protein K48-linked deubiquitination Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of cytokine production involved in inflammatory response Source: Ensembl
Regulation of inflammatory response Source: Ensembl
Response to inorganic substance Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: InterPro

Cellular Location

Nucleus; Cytoplasm. Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).

Involvement in disease

Mesothelioma, malignant (MESOM): An aggressive neoplasm of the serosal lining of the chest. It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos.
Tumor predisposition syndrome (TPDS): A condition characterized by predisposition to develop a variety of tumors, including benign melanocytic tumors as well as several malignant tumors, including uveal melanoma, cutaneous melanoma, malignant mesothelioma on exposure to asbestos, lung adenocarcinoma and meningioma.

PTM

Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention.

Bruno, R., Alì, G., Poma, A. M., Proietti, A., Libener, R., Mariani, N., ... & Fontanini, G. (2020). Differential diagnosis of malignant pleural mesothelioma on cytology: a gene expression panel versus BRCA1-associated protein 1 and p16 tests. The Journal of Molecular Diagnostics, 22(4), 457-466.

Oh, H., Lee, Y. J., Kang, S. G., Ahn, B., Kim, E., Chae, Y. S., ... & Kim, C. H. (2020). BRCA1-associated protein 1 expression and prognostic role in prostate adenocarcinoma. Investigative and clinical urology, 61(2), 166-172.

Okonska, A., Bühler, S., Rao, V., Ronner, M., Blijlevens, M., van der Meulen-Muileman, I. H., ... & Felley-Bosco, E. (2020). Functional genomic screen in mesothelioma reveals that loss of function of BRCA1-associated protein 1 induces chemoresistance to ribonucleotide reductase inhibition. Molecular cancer therapeutics, 19(2), 552-563.

Zhang, A. J., Rush, P. S., Tsao, H., & Duncan, L. M. (2019). BRCA1‐associated protein (BAP1)‐inactivated melanocytic tumors. Journal of cutaneous pathology, 46(12), 965-972.

Glasgow, B. J., & McCannel, T. A. (2018). Correlation of immunocytochemistry of BRCA1-associated protein-1 (BAP1) with other prognostic markers in uveal melanoma. American journal of ophthalmology, 189, 122-126.

Cozzi, I., Oprescu, F. A., Rullo, E., & Ascoli, V. (2018). Loss of BRCA1‐associated protein 1 (BAP 1) expression is useful in diagnostic cytopathology of malignant mesothelioma in effusions. Diagnostic cytopathology, 46(1), 9-14.

Szalai, E., Wells, J. R., Ward, L., & Grossniklaus, H. E. (2018). Uveal melanoma nuclear BRCA1-associated protein-1 immunoreactivity is an indicator of metastasis. Ophthalmology, 125(2), 203-209.

Wang, L. M., Shi, Z. W., Wang, J. L., Lv, Z., Du, F. B., Yang, Q. B., & Wang, Y. (2017). Diagnostic accuracy of BRCA1–associated protein 1 in malignant mesothelioma: a meta-analysis. Oncotarget, 8(40), 68863.

Loeser, H., Waldschmidt, D., Kuetting, F., Schallenberg, S., Zander, T., Bollschweiler, E., ... & Quaas, A. (2017). Somatic BRCA1‑associated protein 1 (BAP1) loss is an early and rare event in esophageal adenocarcinoma. Molecular and clinical oncology, 7(2), 225-228.

Owen, D., Sheffield, B. S., Ionescu, D., & Churg, A. (2017). Loss of BRCA1-associated protein 1 (BAP1) expression is rare in non–small cell lung cancer. Human pathology, 60, 82-85.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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