Human Recombinant BAP1, Active protein, His Tag (V2LY-0526-LY2297)

Name: Human Recombinant BAP1, Active protein, His Tag
SKU: V2LY-0526-LY2297
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant BAP1, Active protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 98 kDa.

Molecule Mass

98 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

BRCA1 Associated Protein 1

Function

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922).
Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (PubMed:20436459, PubMed:25451922).
Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459).
Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555).
Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555).
Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993).
It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993).
Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950).
Acts as a tumor suppressor (PubMed:9528852).

Biological Process

Cellular protein modification process Source: UniProtKB
Double-strand break repair Source: Reactome
Macrophage homeostasis Source: Ensembl
Monoubiquitinated histone H2A deubiquitination Source: UniProtKB
Monoubiquitinated protein deubiquitination Source: UniProtKB
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein deubiquitination Source: UniProtKB
Protein K48-linked deubiquitination Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of cytokine production involved in inflammatory response Source: Ensembl
Regulation of inflammatory response Source: Ensembl
Response to inorganic substance Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: InterPro

Cellular Location

Nucleus; Cytoplasm. Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).

Involvement in disease

Mesothelioma, malignant (MESOM): An aggressive neoplasm of the serosal lining of the chest. It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos.
Tumor predisposition syndrome (TPDS): A condition characterized by predisposition to develop a variety of tumors, including benign melanocytic tumors as well as several malignant tumors, including uveal melanoma, cutaneous melanoma, malignant mesothelioma on exposure to asbestos, lung adenocarcinoma and meningioma.

PTM

Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention.

Bruno, R., Alì, G., Poma, A. M., Proietti, A., Libener, R., Mariani, N., ... & Fontanini, G. (2020). Differential diagnosis of malignant pleural mesothelioma on cytology: a gene expression panel versus BRCA1-associated protein 1 and p16 tests. The Journal of Molecular Diagnostics, 22(4), 457-466.

Oh, H., Lee, Y. J., Kang, S. G., Ahn, B., Kim, E., Chae, Y. S., ... & Kim, C. H. (2020). BRCA1-associated protein 1 expression and prognostic role in prostate adenocarcinoma. Investigative and clinical urology, 61(2), 166-172.

Okonska, A., Bühler, S., Rao, V., Ronner, M., Blijlevens, M., van der Meulen-Muileman, I. H., ... & Felley-Bosco, E. (2020). Functional genomic screen in mesothelioma reveals that loss of function of BRCA1-associated protein 1 induces chemoresistance to ribonucleotide reductase inhibition. Molecular cancer therapeutics, 19(2), 552-563.

Zhang, A. J., Rush, P. S., Tsao, H., & Duncan, L. M. (2019). BRCA1‐associated protein (BAP1)‐inactivated melanocytic tumors. Journal of cutaneous pathology, 46(12), 965-972.

Glasgow, B. J., & McCannel, T. A. (2018). Correlation of immunocytochemistry of BRCA1-associated protein-1 (BAP1) with other prognostic markers in uveal melanoma. American journal of ophthalmology, 189, 122-126.

Cozzi, I., Oprescu, F. A., Rullo, E., & Ascoli, V. (2018). Loss of BRCA1‐associated protein 1 (BAP 1) expression is useful in diagnostic cytopathology of malignant mesothelioma in effusions. Diagnostic cytopathology, 46(1), 9-14.

Szalai, E., Wells, J. R., Ward, L., & Grossniklaus, H. E. (2018). Uveal melanoma nuclear BRCA1-associated protein-1 immunoreactivity is an indicator of metastasis. Ophthalmology, 125(2), 203-209.

Wang, L. M., Shi, Z. W., Wang, J. L., Lv, Z., Du, F. B., Yang, Q. B., & Wang, Y. (2017). Diagnostic accuracy of BRCA1–associated protein 1 in malignant mesothelioma: a meta-analysis. Oncotarget, 8(40), 68863.

Loeser, H., Waldschmidt, D., Kuetting, F., Schallenberg, S., Zander, T., Bollschweiler, E., ... & Quaas, A. (2017). Somatic BRCA1‑associated protein 1 (BAP1) loss is an early and rare event in esophageal adenocarcinoma. Molecular and clinical oncology, 7(2), 225-228.

Owen, D., Sheffield, B. S., Ionescu, D., & Churg, A. (2017). Loss of BRCA1-associated protein 1 (BAP1) expression is rare in non–small cell lung cancer. Human pathology, 60, 82-85.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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