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Mouse Anti-BCAS3 Recombinant Antibody (CBYY-0291) (CBMAB-0292-YY)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBYY-0291
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-300 mapping at the N-terminus of BCAS3 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
breast carcinoma amplified sequence 3
Introduction
Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation (By similarity). Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin.
Entrez Gene ID
54828
UniProt ID
Q9H6U6
Alternative Names
BCAS3, Microtubule Associated Cell Migration Factor; Rudhira; GAOB1; Breast Carcinoma Amplified Sequence 4/3 Fusion Protein; Metastasis Associated Antigen Of Breast Cancer; Breast Carcinoma Amplified Sequence 3;
Function
Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation (By similarity).
Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor-responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin. Plays a regulatory role in autophagic activity. In complex with PHAF1, associates with the preautophagosomal structure during both non-selective and selective autophagy (PubMed:33499712).
Probably binds phosphatidylinositol 3-phosphate (PtdIns3P) which would mediate the recruitment preautophagosomal structures (PubMed:33499712).
Biological Process
Activation of GTPase activity Source: UniProtKB
Angiogenesis Source: UniProtKB-KW
Cellular response to estrogen stimulus Source: UniProtKB
Golgi organization Source: UniProtKB
Microtubule organizing center organization Source: UniProtKB
Negative regulation of focal adhesion assembly Source: UniProtKB
Negative regulation of GTPase activity Source: UniProtKB
Positive regulation of actin cytoskeleton reorganization Source: UniProtKB
Positive regulation of catalytic activity Source: UniProtKB
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of filopodium assembly Source: UniProtKB
Positive regulation of GTPase activity Source: UniProtKB
Positive regulation of intracellular protein transport Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of establishment of cell polarity Source: UniProtKB
Response to starvation Source: GO_Central
Tube formation Source: UniProtKB
Cellular Location
Cytoplasm; Cytoskeleton; Nucleus; Preautophagosomal structure. Localizes in the cytoplasm in stationary cells. Translocates from the cytoplasm to the leading edge in motile cells. Colocalizes with microtubules and intermediate filaments in both stationary and motile cells (By similarity). Associates with chromatin. Recruited to estrogen receptor-induced promoters in a PELP1-dependent manner. The BCAS3:PHAF1 complex is recruited to the preautophagosomal structures adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1 dependent manner (PubMed:33499712).

Hengel, H., Hannan, S. B., Dyack, S., MacKay, S. B., Schatz, U., Fleger, M., ... & Genomics England Research Consortium. (2021). Bi-allelic loss-of-function variants in BCAS3 cause a syndromic neurodevelopmental disorder. The American Journal of Human Genetics, 108(6), 1069-1082.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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