Mouse Anti-BCCIP Recombinant Antibody (CBYY-1938) (CBMAB-2287-YY)

Name: Mouse Anti-BCCIP Recombinant Antibody (CBYY-1938)
SKU: CBMAB-2287-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-1938

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 271-301 near the C-terminus of TOK-1β of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BRCA2 and CDKN1A interacting protein

Introduction

This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforMouse have been described for this gene.

Entrez Gene ID

56647

UniProt ID

Q9P287

Alternative Names

BRCA2 And CDKN1A Interacting Protein; BRCA2 And CDKN1A-Interacting Protein; P21- And CDK-Associated Protein 1; Protein TOK-1; TOK1; BRCA2 And Cip1/P21 Interacting Protein; Cdk Inhibitor P21 Binding Protein;

Function

During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342).
Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta (PubMed:28394342).
May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).

Biological Process

DNA repair Source: UniProtKB-KW
Establishment of mitotic spindle orientation Source: UniProtKB
Microtubule anchoring Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Mitotic spindle assembly Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Neuroendocrine cell differentiation Source: UniProtKB
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI

Cellular Location

Centriole; Spindle pole; Nucleus. Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648). In interphase, preferential localizes to the mother centriole (PubMed:28394342). Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342).
Isoform 1: Centrosome; Spindle pole. Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha.
Isoform 2: Centrosome; Spindle pole. Isoform 2/alpha tends to be more abundant at, and more strongly associated with, centrosomes than isoform 1/beta.

Lu, H., Ye, C., Liu, J., Rabson, A. B., Verzi, M., De, S., & Shen, Z. (2021). Requirement of Bccip for the regeneration of intestinal progenitors. The American Journal of Pathology, 191(1), 66-78.

Shi, J., Lv, X., Li, W., Ming, Z., Zeng, L., Yuan, J., ... & Yang, S. (2021). Overexpression of BCCIP predicts an unfavorable prognosis and promotes the proliferation and migration of lung adenocarcinoma. Thoracic Cancer, 12(17), 2324-2338.

Choi, W. S., Liu, B., Shen, Z., & Yang, W. (2021). Structure of human BCCIP and implications for binding and modification of partner proteins. Protein Science, 30(3), 693-699.

Lu, H., Ye, C., Feng, X., Liu, J., Bhaumik, M., Xia, B., ... & Shen, Z. (2020). Spontaneous development of hepatocellular carcinoma and B-cell lymphoma in mosaic and heterozygous Brca2 and Cdkn1a interacting protein knockout mice. The American journal of pathology, 190(6), 1175-1187.

Sui, Y., Wu, T., Li, F., Wang, F., Cai, Y., & Jin, J. (2019). Yy1/bccip coordinately regulates p53-responsive element (p53re)-mediated transactivation of p21waf1/cip1. International journal of molecular sciences, 20(9), 2095.

Zeng, D., Peng, M., Yang, Q., Yang, C., Liao, Z., Li, Q., ... & Zhao, Y. (2019). The Penaeus stylirostris densovirus capsid protein interacts with the Litopenaeus vannamei BCCIP protein. Fish & shellfish immunology, 88, 198-206.

Sui, Y., Li, F., Wu, T., Ding, J., Lu, Z., Wang, L., ... & Cai, Y. (2018). BCCIP binds to and activates its promoter in a YY 1‐dependent fashion in HCT 116 cells. The FEBS journal, 285(16), 3026-3040.

Droz-Rosario, R., Lu, H., Liu, J., Liu, N. A., Ganesan, S., Xia, B., ... & Shen, Z. (2017). Roles of BCCIP deficiency in mammary tumorigenesis. Breast Cancer Research, 19(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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