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Mouse Anti-BCCIP Recombinant Antibody (CBYY-1938) (CBMAB-2287-YY)

This product is mouse antibody that recognizes BCCIP. The antibody CBYY-1938 can be used for immunoassay techniques such as: Dot
See all BCCIP antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-1938
Antibody Isotype
IgM
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 271-301 near the C-terminus of TOK-1β of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BRCA2 and CDKN1A interacting protein
Introduction
This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforMouse have been described for this gene.
Entrez Gene ID
56647
UniProt ID
Q9P287
Alternative Names
BRCA2 And CDKN1A Interacting Protein; BRCA2 And CDKN1A-Interacting Protein; P21- And CDK-Associated Protein 1; Protein TOK-1; TOK1; BRCA2 And Cip1/P21 Interacting Protein; Cdk Inhibitor P21 Binding Protein;
Function
During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342).
Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta (PubMed:28394342).
May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
Biological Process
DNA repair Source: UniProtKB-KW
Establishment of mitotic spindle orientation Source: UniProtKB
Microtubule anchoring Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Mitotic spindle assembly Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Neuroendocrine cell differentiation Source: UniProtKB
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
Cellular Location
Centriole; Spindle pole; Nucleus. Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648). In interphase, preferential localizes to the mother centriole (PubMed:28394342). Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342).
Isoform 1: Centrosome; Spindle pole. Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha.
Isoform 2: Centrosome; Spindle pole. Isoform 2/alpha tends to be more abundant at, and more strongly associated with, centrosomes than isoform 1/beta.

Lu, H., Ye, C., Liu, J., Rabson, A. B., Verzi, M., De, S., & Shen, Z. (2021). Requirement of Bccip for the regeneration of intestinal progenitors. The American Journal of Pathology, 191(1), 66-78.

Shi, J., Lv, X., Li, W., Ming, Z., Zeng, L., Yuan, J., ... & Yang, S. (2021). Overexpression of BCCIP predicts an unfavorable prognosis and promotes the proliferation and migration of lung adenocarcinoma. Thoracic Cancer, 12(17), 2324-2338.

Choi, W. S., Liu, B., Shen, Z., & Yang, W. (2021). Structure of human BCCIP and implications for binding and modification of partner proteins. Protein Science, 30(3), 693-699.

Lu, H., Ye, C., Feng, X., Liu, J., Bhaumik, M., Xia, B., ... & Shen, Z. (2020). Spontaneous development of hepatocellular carcinoma and B-cell lymphoma in mosaic and heterozygous Brca2 and Cdkn1a interacting protein knockout mice. The American journal of pathology, 190(6), 1175-1187.

Sui, Y., Wu, T., Li, F., Wang, F., Cai, Y., & Jin, J. (2019). Yy1/bccip coordinately regulates p53-responsive element (p53re)-mediated transactivation of p21waf1/cip1. International journal of molecular sciences, 20(9), 2095.

Zeng, D., Peng, M., Yang, Q., Yang, C., Liao, Z., Li, Q., ... & Zhao, Y. (2019). The Penaeus stylirostris densovirus capsid protein interacts with the Litopenaeus vannamei BCCIP protein. Fish & shellfish immunology, 88, 198-206.

Sui, Y., Li, F., Wu, T., Ding, J., Lu, Z., Wang, L., ... & Cai, Y. (2018). BCCIP binds to and activates its promoter in a YY 1‐dependent fashion in HCT 116 cells. The FEBS journal, 285(16), 3026-3040.

Droz-Rosario, R., Lu, H., Liu, J., Liu, N. A., Ganesan, S., Xia, B., ... & Shen, Z. (2017). Roles of BCCIP deficiency in mammary tumorigenesis. Breast Cancer Research, 19(1), 1-13.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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