Human Recombinant BCCIP protein, GST Tag (V2LY-0526-LY2307)

Name: Human Recombinant BCCIP protein, GST Tag
SKU: V2LY-0526-LY2307
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant BCCIP protein, GST Tag consist of Amino Acid: 1-314 and predicts a molecular mass of 64 kDa.

Molecule Mass

64 kDa

Sequence

Amino Acid: 1-314

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

BRCA2 and CDKN1A interacting protein

Function

During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342).
Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta (PubMed:28394342).
May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).

Biological Process

DNA repair Source: UniProtKB-KW
Establishment of mitotic spindle orientation Source: UniProtKB
Microtubule anchoring Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Mitotic spindle assembly Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Neuroendocrine cell differentiation Source: UniProtKB
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI

Cellular Location

Centriole; Spindle pole; Nucleus. Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648). In interphase, preferential localizes to the mother centriole (PubMed:28394342). Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342).
Isoform 1: Centrosome; Spindle pole. Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha.
Isoform 2: Centrosome; Spindle pole. Isoform 2/alpha tends to be more abundant at, and more strongly associated with, centrosomes than isoform 1/beta.

Lu, H., Ye, C., Liu, J., Rabson, A. B., Verzi, M., De, S., & Shen, Z. (2021). Requirement of Bccip for the regeneration of intestinal progenitors. The American Journal of Pathology, 191(1), 66-78.

Shi, J., Lv, X., Li, W., Ming, Z., Zeng, L., Yuan, J., ... & Yang, S. (2021). Overexpression of BCCIP predicts an unfavorable prognosis and promotes the proliferation and migration of lung adenocarcinoma. Thoracic Cancer, 12(17), 2324-2338.

Choi, W. S., Liu, B., Shen, Z., & Yang, W. (2021). Structure of human BCCIP and implications for binding and modification of partner proteins. Protein Science, 30(3), 693-699.

Lu, H., Ye, C., Feng, X., Liu, J., Bhaumik, M., Xia, B., ... & Shen, Z. (2020). Spontaneous development of hepatocellular carcinoma and B-cell lymphoma in mosaic and heterozygous Brca2 and Cdkn1a interacting protein knockout mice. The American journal of pathology, 190(6), 1175-1187.

Sui, Y., Wu, T., Li, F., Wang, F., Cai, Y., & Jin, J. (2019). Yy1/bccip coordinately regulates p53-responsive element (p53re)-mediated transactivation of p21waf1/cip1. International journal of molecular sciences, 20(9), 2095.

Zeng, D., Peng, M., Yang, Q., Yang, C., Liao, Z., Li, Q., ... & Zhao, Y. (2019). The Penaeus stylirostris densovirus capsid protein interacts with the Litopenaeus vannamei BCCIP protein. Fish & shellfish immunology, 88, 198-206.

Sui, Y., Li, F., Wu, T., Ding, J., Lu, Z., Wang, L., ... & Cai, Y. (2018). BCCIP binds to and activates its promoter in a YY 1‐dependent fashion in HCT 116 cells. The FEBS journal, 285(16), 3026-3040.

Droz-Rosario, R., Lu, H., Liu, J., Liu, N. A., Ganesan, S., Xia, B., ... & Shen, Z. (2017). Roles of BCCIP deficiency in mammary tumorigenesis. Breast Cancer Research, 19(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BCCIP protein (CAT#: V2LY-0526-LY2306)

Related Products

Mouse Anti-BCCIP Recombinant Antibody (CBYY-1938) (CAT#: CBMAB-2287-YY)

Human Recombinant BCCIP protein (CAT#: V2LY-0526-LY2306)

Isotype control

For research use only. Not intended for any clinical use.

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