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Mouse Anti-BCR Recombinant Antibody (CBYY-0455) (CBMAB-0458-YY)

This product is mouse antibody that recognizes BCR. The antibody CBYY-0455 can be used for immunoassay techniques such as: WB, IP, IF, ELISA
See all BCR antibodies
Published Data
Mouse Anti-BCR Recombinant Antibody (CBYY-0455) (CBMAB-0458-YY) in IP
Left panel shows tyrosine-phosphorylation of p85 PI3K in HL-60.Bcr ± Abl observed in the same blot. ...View More
Amarante-Mendes, G. P., Jascur, T., Nishioka, W. K., Mustelin, T., & Green, D. R. (1997). Bcr-Abl-mediated resistance to apoptosis is independent of PI 3-kinase activity. Cell Death & Differentiation, 4(7), 548-554.
Mouse Anti-BCR Recombinant Antibody (CBYY-0455) (CBMAB-0458-YY) in IP
The indicated cell lysates were subjected to anti-Runx1 or IgG immunoprecipitation and immunoblotted using the
indicated antibodies. ...View More
Liu, H., Cui, Y., Wang, G. F., Dong, Q., Yao, Y., Li, P., ... & Liu, X. (2018). The nonreceptor tyrosine kinase c-Abl phosphorylates Runx1 and regulates Runx1-mediated megakaryocyte maturation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1865(8), 1060-1072.
Mouse Anti-BCR Recombinant Antibody (CBYY-0455) (CBMAB-0458-YY) in IF
K562 cells were treated with imatinib (2 mM for 48 hrs) in the presence and absence of 3-MA (5 mM). Following treatment, these cells were assessed for expression of Bcr-Abl (red-left panels), LC3 (green-second set of panels from the left), and co-localization of Bcr-Abl and LC3 (third set of panels from the left) using immunofluorescence. To enable the visualization of the co-localization images more clearly, Bcr-Abl and LC3 images are merged without the nuclei (right set of panels). ...View More
Elzinga, B. M., Nyhan, M. J., Crowley, L. C., O'Donovan, T. R., Cahill, M. R., & McKenna, S. L. (2013). Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. American journal of hematology, 88(6), 455-462.
Mouse Anti-BCR Recombinant Antibody (CBYY-0455) (CBMAB-0458-YY) in IP
Western blot analyses of proteins extracted from PLAT-gp cells were performed with an anti-BCR antibody (a) or with an anti-IKAROS antibody (b) (see Materials and methods). The expression of α-tubulin served as a loading control. ...View More
Suzuki, K., Ono, R., Ohishi, K., Masuya, M., Kataoka, I., Liu, B., ... & Nosaka, T. (2012). IKAROS isoform 6 enhances BCR-ABL1-mediated proliferation of human CD34+ hematopoietic cells on stromal cells. International journal of oncology, 40(1), 53-62.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0455
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Synthetic peptide from the amino acid sequence of the Bcr gene product.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BCR, RhoGEF And GTPase Activating Protein
Introduction
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforMouse have been found for this gene.
Entrez Gene ID
Human613
Mouse110279
Rat309696
UniProt ID
HumanP11274
MouseQ6PAJ1
RatF1LXF1
Alternative Names
BCR, RhoGEF And GTPase Activating Protein; Renal Carcinoma Antigen NY-REN-26; Breakpoint Cluster Region; EC 2.7.11.1; D22S11; BCR1; Breakpoint Cluster Region Protein;
Function
Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed:7479768, PubMed:1903516, PubMed:17116687).
The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed:7479768, PubMed:23940119).
The amino terminus contains an intrinsic kinase activity (PubMed:1657398).
Functions as an important negative regulator of neuronal RAC1 activity (By similarity).
Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687).
Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed:23940119).
Biological Process
Activation of GTPase activity Source: UniProtKB
Focal adhesion assembly Source: UniProtKB
Keratinocyte differentiation Source: UniProtKB
Modulation of chemical synaptic transmission Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: Ensembl
Protein autophosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: UniProtKB
Cellular Location
Postsynaptic density; Dendritic spine; Axon; Synapse
Involvement in disease
Leukemia, chronic myeloid (CML): A clonal myeloproliferative disorder of a pluripotent stem cell with a specific cytogenetic abnormality, the Philadelphia chromosome (Ph), involving myeloid, erythroid, megakaryocytic, B-lymphoid, and sometimes T-lymphoid cells, but not marrow fibroblasts.
A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
PTM
Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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