Human Recombinant BCR protein, GST Tag (V2LY-0526-LY2331)

Name: Human Recombinant BCR protein, GST Tag
SKU: V2LY-0526-LY2331
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant BCR protein, GST Tag consist of Amino Acid: 1-695 and predicts a molecular mass of 120 kDa.

Molecule Mass

120 kDa

Sequence

Amino Acid: 1-695

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

BCR, RhoGEF And GTPase Activating Protein

Function

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed:7479768, PubMed:1903516, PubMed:17116687).
The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed:7479768, PubMed:23940119).
The amino terminus contains an intrinsic kinase activity (PubMed:1657398).
Functions as an important negative regulator of neuronal RAC1 activity (By similarity).
Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687).
Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed:23940119).

Biological Process

Activation of GTPase activity Source: UniProtKB
Focal adhesion assembly Source: UniProtKB
Keratinocyte differentiation Source: UniProtKB
Modulation of chemical synaptic transmission Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: Ensembl
Protein autophosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: UniProtKB

Cellular Location

Postsynaptic density; Dendritic spine; Axon; Synapse

Involvement in disease

Leukemia, chronic myeloid (CML): A clonal myeloproliferative disorder of a pluripotent stem cell with a specific cytogenetic abnormality, the Philadelphia chromosome (Ph), involving myeloid, erythroid, megakaryocytic, B-lymphoid, and sometimes T-lymphoid cells, but not marrow fibroblasts.
A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

PTM

Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.

Chen, J. A., Hou, Y., Roskin, K. M., Arber, D. A., Bangs, C. D., Baughn, L. B., ... & Gotlib, J. (2021). Lymphoid blast transformation in an MPN with BCR-JAK2 treated with ruxolitinib: putative mechanisms of resistance. Blood Advances, 5(17), 3492-3496.

Scott, S., Cartwright, A., Francis, S., Whitby, L., Sanzone, A. P., Mulder, A., ... & Chantry, A. (2021). Assessment of droplet digital polymerase chain reaction for measuring BCR‐ABL1 in chronic myeloid leukaemia in an international interlaboratory study. British journal of haematology.

Mokhtar, A. M. A., & binti Hashim, I. F. (2021). Small Rho GTPases and their associated RhoGEFs mutations promote immunological defects in Primary Immunodeficiencies. The International Journal of Biochemistry & Cell Biology, 106034.

Ciccarelli, B. T., Hu, T., Wang, Q., Kim, J. J., & Whitehead, I. P. (2020). Examination of clinically-derived p210 BCR/ABL1 RhoGEF mutations in a murine bone marrow transplantation model of CML. Leukemia Research, 97, 106440.

Peiris, M. N., Li, F., & Donoghue, D. J. (2019). BCR: a promiscuous fusion partner in hematopoietic disorders. Oncotarget, 10(28), 2738.

Mousinho, F., Azevedo, A. P., Mendes, T., Santos, P. S. E., Cerqueira, R., Matos, S., ... & Lima, F. (2018). Concomitant presence of JAK2V617F mutation and BCR‑ABL translocation in two patients: A new entity or a variant of myeloproliferative neoplasms (Case report). Molecular medicine reports, 18(1), 1001-1006.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BCR & RET, Active protein, GST Tag (CAT#: V2LY-0526-LY2330)

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For research use only. Not intended for any clinical use.

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