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Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN)

This product is a Mouse antibody that recognizes BCRP1. The antibody 10B1436 can be used for immunoassay techniques such as: IHC, WB.
See all BCRP1 antibodies
Published Data
Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in FC
The cell apop‑ totic rate of HepG2, HepG2/pcDNA3.1, HepG2/ADM and HepG2/ABCG2 cells was detected by flow cytometry. ...View More
Li, Y., Ding, B., Liu, J., Li, X., He, C., Wang, J., & Liu, L. (2023). Drug resistance of hepatoma cells induced by ATP‑binding cassette transporter G2 by reducing intracellular drug concentration. Experimental and Therapeutic Medicine, 25(3), 1-8.
Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB
The expression level of P-gp, MRP1 and BCRP were compared between KB and KB/VCR cells. ...View More
Zhu, H., Liu, Z., Tang, L., Liu, J., Zhou, M., Xie, F., ... & Yu, L. (2012). Reversal of P-gp and MRP1-mediated multidrug resistance by H6, a gypenoside aglycon from Gynostemma pentaphyllum, in vincristine-resistant human oral cancer (KB/VCR) cells. European journal of pharmacology, 696(1-3), 43-53.
Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB
Effect of H6 on the expression of P-gp, MRP1 and ABCG2 were measured in KB/VCR cells. KB/VCR cells were treated with the described concentration of H6 for 36 h and 48 h, the total RNA and protein were extracted, the mRNA and protein levels of P-gp, MRP1 and BCRP were detected using Quantitative real-time PCR and western blot. ...View More
Zhu, H., Liu, Z., Tang, L., Liu, J., Zhou, M., Xie, F., ... & Yu, L. (2012). Reversal of P-gp and MRP1-mediated multidrug resistance by H6, a gypenoside aglycon from Gynostemma pentaphyllum, in vincristine-resistant human oral cancer (KB/VCR) cells. European journal of pharmacology, 696(1-3), 43-53.
Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB
Analysis of BCRP expression in BCRP-mediated drug-resistant cells (A) and intrinsically BCRP-expressing cells (B). Protein extracts prepared from the cells were subjected to Western blotting as described in
Materials and Methods. ...View More
Yamazaki, R., Nishiyama, Y., Furuta, T., Hatano, H., Igarashi, Y., Asakawa, N., ... & Sugimoto, Y. (2011). Novel acrylonitrile derivatives, YHO-13177 and YHO-13351, reverse BCRP/ABCG2-mediated drug resistance in vitro and in vivo. Molecular cancer therapeutics, 10(7), 1252-1263.
Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB and FC
Effect of YHO-13177 on expression levels of BCRP protein. A, dose dependence of effect of YHO-13177 on expression levels of BCRP protein. HCT116 and HCT116/ BCRP cells, or A549 and A549/SN4 cells were cultured in the absence or presence of YHO13177 for 96 hours. B, time dependence of effect of YHO13177 on expression levels of BCRP protein. HCT116 and HCT116/BCRP cells were cultured in the presence of 1
mmol/L YHO-13177 for various times. Protein extracts prepared from the cells were subjected to Western blotting. The amount of BCRP was semi-quantified by densitometric analysis and calculated relative to that in control cells after normalizing for the amount of a-tubulin. C, effect of YHO-13177 on cell surface expression levels of BCRP protein. HCT116 and HCT116/ BCRP cells were cultured in the presence of 1 mmol/L YHO-13177 for various times, and the cell surface expression of BCRP was analyzed by flow cytometry. ...View More
Yamazaki, R., Nishiyama, Y., Furuta, T., Hatano, H., Igarashi, Y., Asakawa, N., ... & Sugimoto, Y. (2011). Novel acrylonitrile derivatives, YHO-13177 and YHO-13351, reverse BCRP/ABCG2-mediated drug resistance in vitro and in vivo. Molecular cancer therapeutics, 10(7), 1252-1263.

Summary

Host Animal
Mouse
Specificity
Human
Clone
5D3
Antibody Isotype
IgG2b, κ
Application
IP, IF, FC

Basic Information

Immunogen
3T3 cells transfected with recombinant ABCG2 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:50-1:500
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml

Target

Full Name
Breakpoint Cluster Region Pseudogene 1
Introduction
BCRP1 (Breakpoint Cluster Region Pseudogene 1) is a Pseudogene.
Entrez Gene ID
644079
UniProt ID
Q9UNQ0
Alternative Names
Breakpoint Cluster Region Pseudogene 1; BCR-1;
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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