Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN)

Name: Mouse Anti-BCRP1 Recombinant Antibody (5D3)
SKU: CBMAB-C1093-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in FC

The cell apop‑ totic rate of HepG2, HepG2/pcDNA3.1, HepG2/ADM and HepG2/ABCG2 cells was detected by flow cytometry. ...View More

Li, Y., Ding, B., Liu, J., Li, X., He, C., Wang, J., & Liu, L. (2023). Drug resistance of hepatoma cells induced by ATP‑binding cassette transporter G2 by reducing intracellular drug concentration. Experimental and Therapeutic Medicine, 25(3), 1-8.

Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB

The expression level of P-gp, MRP1 and BCRP were compared between KB and KB/VCR cells. ...View More

Zhu, H., Liu, Z., Tang, L., Liu, J., Zhou, M., Xie, F., ... & Yu, L. (2012). Reversal of P-gp and MRP1-mediated multidrug resistance by H6, a gypenoside aglycon from Gynostemma pentaphyllum, in vincristine-resistant human oral cancer (KB/VCR) cells. European journal of pharmacology, 696(1-3), 43-53.

Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB

Effect of H6 on the expression of P-gp, MRP1 and ABCG2 were measured in KB/VCR cells. KB/VCR cells were treated with the described concentration of H6 for 36 h and 48 h, the total RNA and protein were extracted, the mRNA and protein levels of P-gp, MRP1 and BCRP were detected using Quantitative real-time PCR and western blot. ...View More

Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB

Analysis of BCRP expression in BCRP-mediated drug-resistant cells (A) and intrinsically BCRP-expressing cells (B). Protein extracts prepared from the cells were subjected to Western blotting as described in
Materials and Methods. ...View More

Yamazaki, R., Nishiyama, Y., Furuta, T., Hatano, H., Igarashi, Y., Asakawa, N., ... & Sugimoto, Y. (2011). Novel acrylonitrile derivatives, YHO-13177 and YHO-13351, reverse BCRP/ABCG2-mediated drug resistance in vitro and in vivo. Molecular cancer therapeutics, 10(7), 1252-1263.

Mouse Anti-BCRP1 Recombinant Antibody (5D3) (CBMAB-C1093-CN) in WB and FC

Effect of YHO-13177 on expression levels of BCRP protein. A, dose dependence of effect of YHO-13177 on expression levels of BCRP protein. HCT116 and HCT116/ BCRP cells, or A549 and A549/SN4 cells were cultured in the absence or presence of YHO13177 for 96 hours. B, time dependence of effect of YHO13177 on expression levels of BCRP protein. HCT116 and HCT116/BCRP cells were cultured in the presence of 1
mmol/L YHO-13177 for various times. Protein extracts prepared from the cells were subjected to Western blotting. The amount of BCRP was semi-quantified by densitometric analysis and calculated relative to that in control cells after normalizing for the amount of a-tubulin. C, effect of YHO-13177 on cell surface expression levels of BCRP protein. HCT116 and HCT116/ BCRP cells were cultured in the presence of 1 mmol/L YHO-13177 for various times, and the cell surface expression of BCRP was analyzed by flow cytometry. ...View More

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5D3

Application

IP, IF, FC

Immunogen

3T3 cells transfected with recombinant ABCG2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:50-1:500
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Breakpoint Cluster Region Pseudogene 1

Introduction

BCRP1 (Breakpoint Cluster Region Pseudogene 1) is a Pseudogene.

Entrez Gene ID

644079

UniProt ID

Q9UNQ0

Alternative Names

Breakpoint Cluster Region Pseudogene 1; BCR-1;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CAT#: CBMAB-0461-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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