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Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CBMAB-0461-YY)

This product is mouse antibody that recognizes BCRP1. The antibody CBYY-0458 can be used for immunoassay techniques such as: WB, IP, IF, ELISA
See all BCRP1 antibodies
Published Data
Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CBMAB-0461-YY) in WB
Western blotting analysis of ABCG2 and ABCC1 expressions. ...View More
Sabet, Z., Vagiannis, D., Budagaga, Y., Zhang, Y., Novotná, E., Hanke, I., ... & Hofman, J. (2022). Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study. International Journal of Molecular Sciences, 23(22), 14338.
Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CBMAB-0461-YY) in WB
A375 cells were incubated with dHL60-CM, obtained from dHL60 cells previously exposed to CSC-CM for 24 h. After 7 days, Western blot analysis was carried out to analyze the expression of ABCG2. ...View More
Anselmi, M., Fontana, F., Marzagalli, M., Gagliano, N., Sommariva, M., & Limonta, P. (2022). Melanoma Stem Cells Educate Neutrophils to Support Cancer Progression. Cancers, 14(14), 3391.
Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CBMAB-0461-YY) in Immunoblot
A549 cells were transduced with a non-targeting (scrambled) CRISPR-Cas vector or with a CRISPR-Cas vector to knock-out (KO) MRP1 or BCRP. The indicated proteins were measured by immunoblotting. Tubulin was used as control of equal protein loading. The image is representative of one out of three experiments. ...View More
Campani, V., Salaroglio, I. C., Nele, V., Kopecka, J., Bernkop-Schnürch, A., Riganti, C., & De Rosa, G. (2022). Targeted self-emulsifying drug delivery systems to restore docetaxel sensitivity in resistant tumors. Pharmaceutics, 14(2), 292.
Mouse Anti-BCRP1 Recombinant Antibody (CBYY-0458) (CBMAB-0461-YY) in WB
The expression of drug-resistance-related proteins in naive cells and cisplatin cells of Hela was determined by western blotting. ...View More
Li, Y., Wang, J., Gao, C., Hu, Q., & Mao, X. (2021). Integral membrane protein 2A enhances sensitivity to chemotherapy via notch signaling pathway in cervical cancer. Bioengineered, 12(2), 10183-10193.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0458
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 301-370 mapping within an internal region of ABCG2 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Breakpoint Cluster Region Pseudogene 1
Introduction
BCRP1 (Breakpoint Cluster Region Pseudogene 1) is a Pseudogene.
Entrez Gene ID
644079
UniProt ID
Q9UNQ0
Alternative Names
Breakpoint Cluster Region Pseudogene 1; BCR-1;
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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