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Mouse Anti-BDP1 Recombinant Antibody (CBYJT-1047) (CBMAB-T0054-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to BDP1 (B Double Prime 1, Subunit Of RNA Polymerase III Transcription Initiation Factor IIIB). The antibody can be used for immunoassay techniques, such as WB, IHC-P, Dot.
See all BDP1 antibodies
Published Data
Mouse Anti-BDP1 Recombinant Antibody (CBYJT-1047) (CBMAB-T0054-YJ) in WB
BT474 cells were cultured in the absence or in the presence of the indicated concentrations of lapatinib, bortezomib or both drugs together (left panel) or BT474 cells were cultured in the absence or presence of the indicated concentrations of lapatinib, carfilzomib or in combination (right panel). After 30 hours cells were harvested. Western blots of the protein lysates were probed with the indicated antibodies. ...View More
Thaler, S., Schmidt, M., Roβwag, S., Thiede, G., Schad, A., & Sleeman, J. P. (2017). Proteasome inhibitors prevent bi-directional HER2/estrogen-receptor cross-talk leading to cell death in endocrine and lapatinib-resistant HER2+/ER+ breast cancer cells. Oncotarget, 8(42), 72281.
Mouse Anti-BDP1 Recombinant Antibody (CBYJT-1047) (CBMAB-T0054-YJ) in WB
MCF7 cells stably expressing HER2 wt or HER2 insYVMA and negative parental control cells were cultured with the indicated carfilzomib and bortezomib concentrations for 32 hours. Western blots of protein lysates were probed with the indicated antibodies. ...View More
Thaler, S., Schmidt, M., Roβwag, S., Thiede, G., Schad, A., & Sleeman, J. P. (2017). Proteasome inhibitors prevent bi-directional HER2/estrogen-receptor cross-talk leading to cell death in endocrine and lapatinib-resistant HER2+/ER+ breast cancer cells. Oncotarget, 8(42), 72281.
Mouse Anti-BDP1 Recombinant Antibody (CBYJT-1047) (CBMAB-T0054-YJ) in WB
BT474 cells were cultured in the presence of 250 nM carfilzomib or 25 nM bortezomib. Cells were harvested at the indicated time points. Western blots of protein lysates were probed with the indicated antibodies. ...View More
Thaler, S., Schmidt, M., Roβwag, S., Thiede, G., Schad, A., & Sleeman, J. P. (2017). Proteasome inhibitors prevent bi-directional HER2/estrogen-receptor cross-talk leading to cell death in endocrine and lapatinib-resistant HER2+/ER+ breast cancer cells. Oncotarget, 8(42), 72281.
Mouse Anti-BDP1 Recombinant Antibody (CBYJT-1047) (CBMAB-T0054-YJ) in WB
(A) BT474 and (B) MDA-MB-361 cells were cultured in the presence of the indicated carfilzomib (right panels) and bortezomib (left panels) concentrations for 32 hours. Western blots of protein lysates were probed with the indicated antibodies. ...View More
Thaler, S., Schmidt, M., Roβwag, S., Thiede, G., Schad, A., & Sleeman, J. P. (2017). Proteasome inhibitors prevent bi-directional HER2/estrogen-receptor cross-talk leading to cell death in endocrine and lapatinib-resistant HER2+/ER+ breast cancer cells. Oncotarget, 8(42), 72281.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJT-1047
Antibody Isotype
IgG1, λ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 241-453 mapping at the C-terminus of BDP1 of mouse origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, λ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Internal region

Target

Full Name
B Double Prime 1, Subunit Of RNA Polymerase III Transcription Initiation Factor IIIB
Introduction
BDP1 is a subunit of the TFIIIB transcription initiation complex, which recruits RNA polymerase III to target promoters in order to initiate transcription. BDP1 localizes to concentrated aggregates in the nucleus, and is required for transcription from all three types of polymerase III promoters. It is phosphorylated by casein kinase II during mitosis, resulting in its release from chromatin and suppression of polymerase III transcription.
Entrez Gene ID
55814
UniProt ID
A6H8Y1
Alternative Names
B Double Prime 1, Subunit Of RNA Polymerase III Transcription Initiation Factor IIIB; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase III, GTF3B Subunit 1; Transcription Factor-Like Nuclear Regulator; Transcription Factor IIIB 150; TFIIIB150; TFNR; RNA Polymerase III Transcription Initiation Factor B; Transcription Factor TFIIIB Component B Homolog
Function
General activator of RNA polymerase III transcription. Requires for transcription from all three types of polymerase III promoters. Requires for transcription of genes with internal promoter elements and with promoter elements upstream of the initiation site.
Biological Process
RNA polymerase III preinitiation complex assembly Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
Deafness, autosomal recessive, 112 (DFNB112): A form of non-syndromic, sensorineural deafness characterized by postlingual progressive hearing impairment. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
PTM
Phosphorylated by CSNK2A1 during mitosis, resulting in its release from chromatin and suppression of polymerase III transcription.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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