Mouse Anti-BEST3 Recombinant Antibody (CBYY-1896) (CBMAB-2245-YY)

Name: Mouse Anti-BEST3 Recombinant Antibody (CBYY-1896)
SKU: CBMAB-2245-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYY-1896

Application

WB, IF, FC

Immunogen

Full length human recombinant protein of human BEST3 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IF(ICC)	1:100
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bestrophin 3

Introduction

BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron Bovineundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).

Entrez Gene ID

144453

UniProt ID

Q8N1M1

Alternative Names

Bestrophin 3; Vitelliform Macular Dystrophy 2-Like Protein 3; Vitelliform Macular Dystrophy 2-Like 3; VMD2L3; Bestrophin-3;

Function

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Biological Process

Chloride transport Source: UniProtKB-KW
Negative regulation of ion transport Source: Ensembl

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-25 aa
Helical: 26-46 aa
Extracellular: 47-70 aa
Helical: 71-91 aa
Cytoplasmic: 92-178 aa
Helical: 179-199 aa
Extracellular: 200-228 aa
Intramembrane: 229-249 aa
Extracellular: 250-270 aa
Helical: 271-291 aa
Cytoplasmic: 292-668 aa

Wang, X., Zhang, G., Zhu, C., Lin, L., Zhao, Z., Yu, X., ... & Wang, J. (2019). Vitamin C Prevents Hydrocortisone-Induced Injury in HMEC-1 through Promoting Bestrophin-3 Expression. Nutrition and cancer, 71(5), 852-860.

Golubinskaya, V., Vontell, R., Supramaniam, V., Wyatt-Ashmead, J., Gustafsson, H., Mallard, C., & Nilsson, H. (2019). Bestrophin-3 expression in a subpopulation of astrocytes in the neonatal brain after hypoxic-ischemic injury. Frontiers in physiology, 10, 23.

Kajii, T. S., Oka, A., Saito, F., Mitsui, J., & Iida, J. (2019). Whole-exome sequencing in a Japanese pedigree implicates a rare non-synonymous single-nucleotide variant in BEST3 as a candidate for mandibular prognathism. Bone, 122, 193-198.

Singh, S., Wang, Z., Shahin, M., Langaee, T. Y., Gong, Y., Turner, S. T., ... & Johnson, J. A. (2018). Targeted Sequencing Identifies Missense variant in the BEST3 gene associated with Antihypertensive Response to Hydrochlorothiazide. Pharmacogenetics and genomics, 28(11), 251.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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