Mouse Anti-BET1 Recombinant Antibody (CBYY-0503) (CBMAB-0506-YY)

Name: Mouse Anti-BET1 Recombinant Antibody (CBYY-0503)
SKU: CBMAB-0506-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYY-0503

Application

WB, IP, IF, ELISA

Immunogen

Full length Bet1 of rat origin.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bet1 Golgi Vesicular Membrane Trafficking Protein

Introduction

This gene encodes a golgi-associated membrane protein that participates in vesicular transport from the endoplasmic reticulum (ER) to the Golgi complex. The encoded protein functions as a soluble N-ethylaleimide-sensitive factor attacHumanent protein receptor and may be involved in the docking of ER-derived vesicles with the cis-Golgi membrane. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Mouse	10282
Rat	29631

UniProt ID

Mouse	O35623
Rat	Q62896

Alternative Names

Bet1 Golgi Vesicular Membrane Trafficking Protein; Golgi Vesicular Membrane Trafficking Protein P18; Golgi Vesicular Membrane-Trafficking Protein P18; Bet1p Homolog; HBET1; Blocked Early In Transport 1 Homolog (S. Cerevisiae);

Function

Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane (By similarity).

Biological Process

COPII vesicle coating Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Protein transport Source: UniProtKB-KW
Vesicle fusion with Golgi apparatus Source: GO_Central

Cellular Location

Cis-Golgi network membrane; Golgi apparatus membrane; Endoplasmic reticulum membrane. Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus (By similarity).

Topology

Cytoplasmic: 1-94 aa
Helical: 95-115 aa
Vesicular: 116-118 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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