Rabbit Anti-BIRC6 Recombinant Antibody (D8B5) (CBMAB-CP0002-LY)

Name: Rabbit Anti-BIRC6 Recombinant Antibody (D8B5)
SKU: CBMAB-CP0002-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D8B5

Application

Immunogen

Synthetic peptide corresponding to residues surrounding Asp147 in human BIRC6 protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Hamster, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

baculoviral IAP repeat containing 6

Introduction

This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.

Entrez Gene ID

Human	57448
Mouse	12211
Rat	313876
Hamster	100689237
Monkey	709443

UniProt ID

Human	Q9NR09
Mouse	O88738
Rat	F1LY70
Hamster	A0A1U7QV43
Monkey	A0A2K5UQ74

Alternative Names

Baculoviral IAP Repeat Containing 6; Apollon; BIR Repeat-Containing Ubiquitin-Conjugating Enzyme; RING-Type E3 Ubiquitin Transferase BIRC6; BRUCE; Ubiquitin-Conjugating BIR-Domain Enzyme Apollon;

Function

Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Labyrinthine layer development Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Protein phosphorylation Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of cell population proliferation Source: UniProtKB
Regulation of cytokinesis Source: UniProtKB
Spongiotrophoblast layer development Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Trans-Golgi network membrane; Spindle pole; Centrosome; Endosome; Midbody ring. Exhibits cell cycle-dependent localization. Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis. On the midbody, localizes to the midbody ring, also called Flemming body (PubMed:18329369). In interphase cells, localizes to the trans-Golgi network membrane and endosomes. During cytokinesis, a fraction moves to the midzone where it specifically arrives at the midbody ring. After abscission completion, travels with the midbody remnant into one daughter cell, and remains bound to it until a new midbody ring is formed during the next cell division (PubMed:18329369).

PTM

Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY.

Bergna, S. M. G., Marchesini, A., Morales, L. C. A., Arrías, P. N., Farina, H. G., Romanowski, V., ... & Pidre, M. L. (2021). Exploring the metastatic role of the inhibitor of apoptosis BIRC6 in Breast Cancer. bioRxiv.

SM, G. B., Marchesini, A., LC, A. M., Arrias, P. N., Farina, H. G., Romanowski, V., ... & Pidre, M. L. (2021). Exploring the metastatic role of the inhibitor of apoptosis BIRC6 in Breast Cancer.

Hao, T., Wang, Z., Yang, J., Zhang, Y., Shang, Y., & Sun, J. (2020). MALAT1 knockdown inhibits prostate cancer progression by regulating miR-140/BIRC6 axis. Biomedicine & Pharmacotherapy, 123, 109666.

Liu, Y., Guo, J., Shen, K., Wang, R., Chen, C., Liao, Z., & Zhou, J. (2020). Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/YWHAZ Axis. OncoTargets and therapy, 13, 9377.

Jin, Z., Gao, B., Gong, Y., & Guan, L. (2020). Depletion of circ-BIRC6, a circular RNA, suppresses non-small cell lung cancer progression by targeting miR-4491. BioScience Trends.

Yang, G., Wang, X., Liu, B., Lu, Z., Xu, Z., Xiu, P., ... & Li, J. (2019). circ-BIRC6, a circular RNA, promotes hepatocellular carcinoma progression by targeting the miR-3918/Bcl2 axis. Cell Cycle, 18(9), 976-989.

Wang, Z., Luo, H., Fang, Z., Fan, Y., Liu, X., Zhang, Y., ... & Zhang, M. (2018). MiR-204 acts as a potential therapeutic target in acute myeloid leukemia by increasing BIRC6-mediated apoptosis. BMB reports, 51(9), 444.

Zhuang, W., Zhang, C., Hao, F., & Sun, X. (2018). Baculoviral IAP repeat containing 6 (BIRC6) is a predictor of prognosis in prostate cancer. Medical science monitor: international medical journal of experimental and clinical research, 24, 839.

Ikeda, F. (2018). The anti-apoptotic ubiquitin conjugating enzyme BIRC6/BRUCE regulates autophagosome-lysosome fusion. Autophagy, 14(7), 1283-1284.

Okumu, D. O., East, M. P., Levine, M., Herring, L. E., Zhang, R., Gilbert, T. S., ... & Graves, L. M. (2017). BIRC6 mediates imatinib resistance independently of Mcl-1. PLoS One, 12(5), e0177871.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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