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Rabbit Anti-BIRC6 Recombinant Antibody (D8B5) (CBMAB-CP0002-LY)

The product is antibody recognizes BIRC6. The antibody D8B5 immunoassay techniques such as: WB.
See all BIRC6 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Hamster, Monkey
Clone
D8B5
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp147 in human BIRC6 protein.
Specificity
Human, Mouse, Rat, Hamster, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
baculoviral IAP repeat containing 6
Introduction
This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.
Entrez Gene ID
Human57448
Mouse12211
Rat313876
Hamster100689237
Monkey709443
UniProt ID
HumanQ9NR09
MouseO88738
RatF1LY70
HamsterA0A1U7QV43
MonkeyA0A2K5UQ74
Alternative Names
Baculoviral IAP Repeat Containing 6; Apollon; BIR Repeat-Containing Ubiquitin-Conjugating Enzyme; RING-Type E3 Ubiquitin Transferase BIRC6; BRUCE; Ubiquitin-Conjugating BIR-Domain Enzyme Apollon;
Function
Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Labyrinthine layer development Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Protein phosphorylation Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of cell population proliferation Source: UniProtKB
Regulation of cytokinesis Source: UniProtKB
Spongiotrophoblast layer development Source: Ensembl
Ubiquitin-dependent protein catabolic process Source: GO_Central
Cellular Location
Trans-Golgi network membrane; Spindle pole; Centrosome; Endosome; Midbody ring. Exhibits cell cycle-dependent localization. Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis. On the midbody, localizes to the midbody ring, also called Flemming body (PubMed:18329369). In interphase cells, localizes to the trans-Golgi network membrane and endosomes. During cytokinesis, a fraction moves to the midzone where it specifically arrives at the midbody ring. After abscission completion, travels with the midbody remnant into one daughter cell, and remains bound to it until a new midbody ring is formed during the next cell division (PubMed:18329369).
PTM
Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY.

Bergna, S. M. G., Marchesini, A., Morales, L. C. A., Arrías, P. N., Farina, H. G., Romanowski, V., ... & Pidre, M. L. (2021). Exploring the metastatic role of the inhibitor of apoptosis BIRC6 in Breast Cancer. bioRxiv.

SM, G. B., Marchesini, A., LC, A. M., Arrias, P. N., Farina, H. G., Romanowski, V., ... & Pidre, M. L. (2021). Exploring the metastatic role of the inhibitor of apoptosis BIRC6 in Breast Cancer.

Hao, T., Wang, Z., Yang, J., Zhang, Y., Shang, Y., & Sun, J. (2020). MALAT1 knockdown inhibits prostate cancer progression by regulating miR-140/BIRC6 axis. Biomedicine & Pharmacotherapy, 123, 109666.

Liu, Y., Guo, J., Shen, K., Wang, R., Chen, C., Liao, Z., & Zhou, J. (2020). Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/YWHAZ Axis. OncoTargets and therapy, 13, 9377.

Jin, Z., Gao, B., Gong, Y., & Guan, L. (2020). Depletion of circ-BIRC6, a circular RNA, suppresses non-small cell lung cancer progression by targeting miR-4491. BioScience Trends.

Yang, G., Wang, X., Liu, B., Lu, Z., Xu, Z., Xiu, P., ... & Li, J. (2019). circ-BIRC6, a circular RNA, promotes hepatocellular carcinoma progression by targeting the miR-3918/Bcl2 axis. Cell Cycle, 18(9), 976-989.

Wang, Z., Luo, H., Fang, Z., Fan, Y., Liu, X., Zhang, Y., ... & Zhang, M. (2018). MiR-204 acts as a potential therapeutic target in acute myeloid leukemia by increasing BIRC6-mediated apoptosis. BMB reports, 51(9), 444.

Zhuang, W., Zhang, C., Hao, F., & Sun, X. (2018). Baculoviral IAP repeat containing 6 (BIRC6) is a predictor of prognosis in prostate cancer. Medical science monitor: international medical journal of experimental and clinical research, 24, 839.

Ikeda, F. (2018). The anti-apoptotic ubiquitin conjugating enzyme BIRC6/BRUCE regulates autophagosome-lysosome fusion. Autophagy, 14(7), 1283-1284.

Okumu, D. O., East, M. P., Levine, M., Herring, L. E., Zhang, R., Gilbert, T. S., ... & Graves, L. M. (2017). BIRC6 mediates imatinib resistance independently of Mcl-1. PLoS One, 12(5), e0177871.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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