Mouse Anti-BMP10 Recombinant Antibody (CBYY-0656) (CBMAB-0659-YY)
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Published Data
CBMAB-0659-YY in Neutralization
EC number and proliferation in HHT mice: Effect of palbociclib.
(A) Schematic representation of the i.p. injection protocol. Arrowheads indicate the postnatal days (P) of injection (AM or PM). Pups were euthanized at P6 for analysis. (B) Representative staining using isolectin B4 (IB4, green), and of ERG (blue) and EdU (red) in the peri-optic nerve and mid-plexus regions of retinas from PBS controls, vehicle-treated BMP9/10ib mice, and palbociclib-treated BMP9/10ib mice. Arrows denote AVMs; a, artery; v, vein. Scale bars, 50 μm. (C and D) Scatter plots showing the total number of ERG+ cells per 200 μm2 in arteries (C) and veins (D) in the peri-optic nerve region across three groups: PBS (n=11), vehicle-treated BMP9/10ib (n=9), and palbociclib-treated BMP9/10ib (n=12) mice. (E and F) Scatter plots showing ERG+ EdU+ cells in the peri-optic nerve (E) and mid-plexus (F) regions across three groups: PBS (n=4-6), vehicle-treated BMP9/10ib (n=4-6), and palbociclib-treated BMP9/10ib (n=6) mice. Data represent individual retinas and mean ± SEM, one-way ANOVA with Tukey's multiple comparisons test. ns, not significant; *P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001. ...View More
(A) Schematic representation of the i.p. injection protocol. Arrowheads indicate the postnatal days (P) of injection (AM or PM). Pups were euthanized at P6 for analysis. (B) Representative staining using isolectin B4 (IB4, green), and of ERG (blue) and EdU (red) in the peri-optic nerve and mid-plexus regions of retinas from PBS controls, vehicle-treated BMP9/10ib mice, and palbociclib-treated BMP9/10ib mice. Arrows denote AVMs; a, artery; v, vein. Scale bars, 50 μm. (C and D) Scatter plots showing the total number of ERG+ cells per 200 μm2 in arteries (C) and veins (D) in the peri-optic nerve region across three groups: PBS (n=11), vehicle-treated BMP9/10ib (n=9), and palbociclib-treated BMP9/10ib (n=12) mice. (E and F) Scatter plots showing ERG+ EdU+ cells in the peri-optic nerve (E) and mid-plexus (F) regions across three groups: PBS (n=4-6), vehicle-treated BMP9/10ib (n=4-6), and palbociclib-treated BMP9/10ib (n=6) mice. Data represent individual retinas and mean ± SEM, one-way ANOVA with Tukey's multiple comparisons test. ns, not significant; *P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001. ...View More
Dinakaran, S., Zhao, H., Tang, Y., Wang, Z., Ruiz, S., Nomura-Kitabayashi, A., ... & Marambaud, P. (2023). CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia. bioRxiv.
CBMAB-0659-YY in ELISA
Levels of (a) BMP9 and (b) pBMP10 in plasma were measured with specific ELISAs.
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Hodgson, J., Ruiz-Llorente, L., McDonald, J., Quarrell, O., Ugonna, K., Bentham, J., ... & Upton, P. D. (2021). Homozygous GDF2 nonsense mutations result in a loss of circulating BMP9 and BMP10 and are associated with either PAH or an "HHT-like" syndrome in children. Molecular Genetics & Genomic Medicine, 9(12), e1685.
CBMAB-0659-YY in ELISA
Plasma samples from controls or patients with either pre-cirrhotic liver fibrosis or with cirrhosis were assayed by ELISA for pBMP10. Error bars show median and interquartile range.
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Owen, N. E., Alexander, G. J., Sen, S., Bunclark, K., Polwarth, G., Pepke-Zaba, J., ... & Upton, P. D. (2020). Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis. EBioMedicine, 56.
CBMAB-0659-YY in ELISA
Plasma samples from a separate cohort of controls and patients with confirmed PoPH were assayed by ELISA pBMP10.
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Owen, N. E., Alexander, G. J., Sen, S., Bunclark, K., Polwarth, G., Pepke-Zaba, J., ... & Upton, P. D. (2020). Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis. EBioMedicine, 56.
CBMAB-0659-YY in WB
A, schematic representation of recombinant BMP9 and BMP10 (pro-BMPs), containing respectively a His6 epitope and a myc epitope downstream of the furin-cleavage site. B, Western blot (WB) analysis of CM from HEK-293-transfected cells. HEK-293 cells were stably transfected with plasmids encoding His-BMP9, myc-BMP10, or both plasmids (indicated BMP9-BMP10). Proteins were detected with antibodies directed against the mature domain of BMP9 or BMP10 as indicated. All samples were run under non-reduced conditions. NT, nontransfected cells. C-E, HisTrapTM chromatography of CM from cells cotransfected with His-BMP9/myc-BMP10. C, CM applied under native conditions. D, CM denaturated by 8 mol/liter of urea and heated at 65°C for 20 min before being loaded onto the column. E, CM denatured as above, then reduced by DTT and alkylated before loading. Flow-through (FT) was collected. Columns were washed with 20 mmol/liter of imidazole (wash) and eluted by 200 mmol/liter of imidazole (elution). Fractions were analyzed by Western blot with the indicated antibodies under non-reduced (C and D) or reduced conditions (E). Note that the anti-myc antibody gives a strong non-specific band around 100 kDa (*). In C and E, elution tracks are surrounded by a black line to highlight spliced 10 mm imidazole washing fractions. F and G, heterodimerization of a mutant BMP and a wildtype (WT) counterpart. Fully-cleaved mature WT BMP9 and BMP10 have been produced without any tag sequence. BMP9 and BMP10 furin-cleavage sites have been mutated to create uncleavable sequences, respectively, RQAA and RIAA. Wildtype and mutant BMPs have been solely transfected (0.5 ng of plasmid in each condition), or co-transfected (0.5 ng of plasmid coding for WT BMP and 1.5 ng of mutant BMP) into HEK-293 cells. CM were analyzed by Western blotting with the indicated antibodies directed against mature BMPs (F) or for their BRE activity on ALK1-transfected 3T3 cells (G).
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Tillet, E., Ouarné, M., Desroches-Castan, A., Mallet, C., Subileau, M., Didier, R., ... & Bailly, S. (2018). A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma. Journal of Biological Chemistry, 293(28), 10963-10974.
CBMAB-0659-YY in WB
Western blotting (WB) detection of purified BMP9-10 heterodimer under reduced (BMP9) or unreduced (BMP10) conditions.
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Tillet, E., Ouarné, M., Desroches-Castan, A., Mallet, C., Subileau, M., Didier, R., ... & Bailly, S. (2018). A heterodimer formed by bone morphogenetic protein 9 (BMP9) and BMP10 provides most BMP biological activity in plasma. Journal of Biological Chemistry, 293(28), 10963-10974.
CBMAB-0659-YY in Blocking
Transmammary transfer of BMP9 and BMP10 blocking Abs into the circulation of mouse neonates.
(A-D) ELISAs were performed to measure IgG2a (A,C) and anti-BMP9 Ab (B,D) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with (A,B), or by direct i.p. injections of (C,D), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5-7 pups per group from 2 dams); ****P < 0.0001, ANOVA. ...View More
(A-D) ELISAs were performed to measure IgG2a (A,C) and anti-BMP9 Ab (B,D) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with (A,B), or by direct i.p. injections of (C,D), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5-7 pups per group from 2 dams); ****P < 0.0001, ANOVA. ...View More
Ruiz, S., Zhao, H., Chandakkar, P., Chatterjee, P. K., Papoin, J., Blanc, L., ... & Marambaud, P. (2016). A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10. Scientific reports, 6(1), 37366.
CBMAB-0659-YY in WB
PITX2, IRX3, and BMP10 protein expression level measured by Western blot of total protein lysates.
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Kahr, P. C., Piccini, I., Fabritz, L., Greber, B., Schöler, H., Scheld, H. H., ... & Kirchhof, P. (2011). Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue. PloS one, 6(10), e26389.
Datasheet
Target
References
Q & As
Review & reward
Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
CBYY-0656
Application
N, WB
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-10 Asn317-Arg424.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
| Application | Note |
| WB | 1 µg/ml |
| Neutralization | 0.15-0.9 µg/ml |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 35-84
More Infomation
Target
Full Name
Bone Morphogenetic Protein 10
Introduction
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which binds to the activin receptor-like kinase 1 (ALK1) and plays important roles in cardiovascular development including cardiomyocyte proliferation and regulation of heart size, closure of the ductus arteriosus, angiogenesis and ventricular trabeculation. [provided by RefSeq, Aug 2016]
Entrez Gene ID
UniProt ID
Alternative Names
Bovinene Morphogenetic Protein 10; BMP-10;
Function
Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.
Biological Process
Activin receptor signaling pathway Source: BHF-UCL
Adult heart development Source: BHF-UCL
Atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
BMP signaling pathway Source: BHF-UCL
Cardiac muscle cell proliferation Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Heart trabecula formation Source: BHF-UCL
Kidney development Source: Ensembl
Negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of endothelial cell migration Source: BHF-UCL
Pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Positive regulation of cardiac muscle cell proliferation Source: BHF-UCL
Positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
Positive regulation of cartilage development Source: Ensembl
Positive regulation of cell proliferation involved in heart morphogenesis Source: BHF-UCL
Positive regulation of gene expression Source: Ensembl
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Positive regulation of sarcomere organization Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Regulation of cardiac muscle contraction Source: BHF-UCL
Regulation of cardiac muscle hypertrophy in response to stress Source: BHF-UCL
Sarcomere organization Source: BHF-UCL
SMAD protein signal transduction Source: GO_Central
Ventricular cardiac muscle cell development Source: BHF-UCL
Ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
Adult heart development Source: BHF-UCL
Atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
BMP signaling pathway Source: BHF-UCL
Cardiac muscle cell proliferation Source: UniProtKB
Cell adhesion Source: UniProtKB-KW
Heart trabecula formation Source: BHF-UCL
Kidney development Source: Ensembl
Negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of endothelial cell migration Source: BHF-UCL
Pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Positive regulation of cardiac muscle cell proliferation Source: BHF-UCL
Positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
Positive regulation of cartilage development Source: Ensembl
Positive regulation of cell proliferation involved in heart morphogenesis Source: BHF-UCL
Positive regulation of gene expression Source: Ensembl
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Positive regulation of sarcomere organization Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Regulation of cardiac muscle contraction Source: BHF-UCL
Regulation of cardiac muscle hypertrophy in response to stress Source: BHF-UCL
Sarcomere organization Source: BHF-UCL
SMAD protein signal transduction Source: GO_Central
Ventricular cardiac muscle cell development Source: BHF-UCL
Ventricular cardiac muscle tissue morphogenesis Source: BHF-UCL
Cellular Location
Secreted
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols
For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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