Mouse Anti-BNC1 Recombinant Antibody (1F4) (CBMAB-A0841-LY)

Name: Mouse Anti-BNC1 Recombinant Antibody (1F4)
SKU: CBMAB-A0841-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1F4

Antibody Isotype

IgG2b, κ

Application

WB, E

Basic Information

Immunogen

BNC1 (NP_001708, 599 a.a. ~ 705 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

basonuclin 1

Introduction

The protein encoded by this gene is a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. It is also found in abundance in the germ cells of testis and ovary. This protein is thought to play a regulatory role in keratinocyte proliferation and it may also be a regulator for rRNA transcription. This gene seems to have multiple alternatively spliced transcript variants, but their full-length nature is not known yet. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]

Entrez Gene ID

646

UniProt ID

Q01954

Alternative Names

BNC; BSN1; HsT19447

Function

Transcriptional activator (By similarity).
It is likely involved in the regulation of keratinocytes terminal differentiation in squamous epithelia and hair follicles (PubMed:8034748).
Required for the maintenance of spermatogenesis (By similarity).
It is involved in the positive regulation of oocyte maturation, probably acting through the control of BMP15 levels and regulation of AKT signaling cascade (PubMed:30010909).
May also play a role in the early development of embryos (By similarity).

Biological Process

Cell differentiation Source: UniProtKB-KW
Epidermis development Source: ProtInc
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of oocyte maturation Source: UniProtKB
Positive regulation of transcription by RNA polymerase I Source: ARUK-UCL
Regulation of transcription, DNA-templated Source: GO_Central
Regulation of transcription by RNA polymerase I Source: GO_Central
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus; Nucleoplasm. Relocates to the midpiece of the flagellum during late spermiogenesis in spermatids.

Involvement in disease

Premature ovarian failure 16 (POF16): An autosomal dominant form of premature ovarian failure, an ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

PTM

Phosphorylation on Ser-537 and Ser-541 leads to cytoplasmic localization.

More Infomation

References

Li, J. Y., Ying, Y. Y., Qian, Y. L., Chen, J. P., Huang, Y., Liu, J., ... & Zhang, D. (2021). BNC1 Promotes Spermatogenesis by Regulating Transcription of Ybx2 and Papolb via Direct Binding to Their Promotor Elements. Reproductive Sciences, 28(3), 785-793.

Li, J. Y., Liu, Y. F., Xu, H. Y., Zhang, J. Y., Lv, P. P., Liu, M. E., ... & Huang, H. F. (2020). Basonuclin 1 deficiency causes testicular premature aging: BNC1 cooperates with TAF7L to regulate spermatogenesis. Journal of molecular cell biology, 12(1), 71-83.

McManus, S. (2020). BNC1 regulates human epicardial heterogeneity and function (Doctoral dissertation, University of Cambridge).

Li, X. B., Ma, J., Liu, Z. W., He, W. F., Li, Z. Z., Cui, M., ... & Zhao, Y. P. (2019). Non-invasive detection of pancreatic cancer by measuring DNA methylation of Basonuclin 1 and Septin 9 in plasma. Chinese medical journal, 132(12), 1504.

Zhang, D., Liu, Y., Zhang, Z., Lv, P., Liu, Y., Li, J., ... & Huang, H. (2018). Basonuclin 1 deficiency is a cause of primary ovarian insufficiency. Human molecular genetics, 27(21), 3787-3800.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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