Mouse Anti-BOLL Recombinant Antibody (CBYY-0718) (CBMAB-0721-YY)

Name: Mouse Anti-BOLL Recombinant Antibody (CBYY-0718)
SKU: CBMAB-0721-YY
Rating: 5 (1 reviews)

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Published Data

CBMAB-0721-YY in IF

The developed cultures were collected and examined for the presence of premeiotic cells (VASA), meiotic cells (BOULE) or postmeiotic cells (ACROSIN) by immunofluorescence staining using antibodies specific for each cell marker. ...View More

Sawaied, A., Arazi, E., AbuElhija, A., Lunenfeld, E., & Huleihel, M. (2021). The presence of colony-stimulating factor-1 and its receptor in different cells of the testis; it involved in the development of spermatogenesis in vitro. International Journal of Molecular Sciences, 22(5), 2325.

CBMAB-0721-YY in IHC

The micrographs represent typical histological features to illustrate the parameters for scoring of morphological characteristics in testicular xenografts. PAS stained sections were evaluated for tubular survival (A-D), tubular appearance (E-H), epithelial pattern of Sertoli cells (I-L) and most advanced germ cells (M-P). Blinded scoring of each graft section was performed as depicted in the table. Cellular identity was validated by immuno-histochemical staining. Cells showing intense expression for SOX9, MAGEA4, BOLL, and ACROSIN were identified, indicating the presence of Sertoli cells (I-M), spermatogonia (N), spermatocytes (O), and spermatids (P), respectively. Scale bar 20 μm. Triangles depict representative positive cells for the respective markers. ...View More

Sharma, S., Sandhowe-Klaverkamp, R., & Schlatt, S. (2018). Differentiation of testis xenografts in the prepubertal marmoset depends on the sex and status of the mouse host. Frontiers in Endocrinology, 9, 395226.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-0718

Antibody Isotype

IgG2b, κ

Application

WB, IP, IF, E

Basic Information

Immunogen

Amino acids 111-199 mapping within an internal region of BOULE of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 510-560

Target

Full Name

bol, boule-like (Drosophila)

Introduction

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia).

Entrez Gene ID

66037

UniProt ID

Q8N9W6

Alternative Names

BOULE

Function

Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation (By similarity).

Biological Process

3'-UTR-mediated mRNA stabilization Source: GO_Central
Cell differentiation Source: UniProtKB-KW
Meiotic cell cycle Source: MGI
Multicellular organism development Source: UniProtKB-KW
Positive regulation of translational initiation Source: UniProtKB
Spermatogenesis Source: UniProtKB-KW

Cellular Location

Cytoplasm

More Infomation

References

Kalwar, Q., Chu, M., Ahmad, A. A., Xiong, L., Zhang, Y., Ding, X., & Yan, P. (2021). Expressional Profiling of TEX11, ESRα and BOLL Genes in Yak under Different Feeding Conditions. Biology, 10(8), 731.

Nosková, A., Wurmser, C., Crysnanto, D., Sironen, A., Uimari, P., Fries, R., ... & Pausch, H. (2020). Deletion of porcine BOLL is associated with defective acrosomes and subfertility in Yorkshire boars. Animal Genetics, 51(6), 945-949.

Li, T., Wang, X., Zhang, H., Chen, Z., Zhao, X., & Ma, Y. (2019). Histomorphological comparisons and expression patterns of boll gene in sheep testes at different development stages. Animals, 9(3), 105.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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