Mouse Anti-BRF1 Recombinant Antibody (CBYY-1788) (CBMAB-2137-YY)

Name: Mouse Anti-BRF1 Recombinant Antibody (CBYY-1788)
SKU: CBMAB-2137-YY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-1788

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, E

Basic Information

Immunogen

Amino acids 1-120 mapping at the N-terminus of TFIIIB90 -1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

BRF1, RNA Polymerase III Transcription Initiation Factor Subunit

Introduction

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforMouse, that function at different promoters transcribed by RNA polymerase III, have been identified.

Entrez Gene ID

2972

UniProt ID

Q92994

Alternative Names

BRF1, RNA Polymerase III Transcription Initiation Factor Subunit; TATA Bovinex Binding Protein (TBP)-Associated Factor, RNA Polymerase III, GTF3B Subunit 2; BRF1, RNA Polymerase III Transcription Initiation Factor 90 KDa Subunit; TFIIIB90; TAF3B2; GTF3B; TAF3C; BRF-1; HBRF; BRF; BRF1 Homolog, Subunit Of RNA Polymerase III Transcription Initiation Factor IIIB (S. Cerevisiae); BRF1 Homolog, Subunit Of RNA Polymerase III Transcription Initiation Factor IIIB; TATA Bovinex-Binding Protein-Associated Factor, RNA Polymerase III, Subunit 2;

Function

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Biological Process

DNA-templated transcription, initiation Source: GO_Central
Positive regulation of transcription by RNA polymerase III Source: Ensembl
rRNA transcription Source: ProtInc
Transcription by RNA polymerase III Source: GO_Central
Transcription initiation from RNA polymerase III promoter Source: ProtInc
Transcription preinitiation complex assembly Source: InterPro
tRNA transcription Source: ProtInc

Cellular Location

Nucleus

Involvement in disease

Cerebellofaciodental syndrome (CFDS): An autosomal recessive disorder characterized by cerebellar hypoplasia, delayed development and intellectual disability, as well as facial dysmorphic features, short stature, microcephaly, and dental anomalies.

More Infomation

References

Florencio-Martínez, L. E., Cano-Santiago, A., Mondragón-Rosas, F., Gómez-García, M., Flores-Pérez, C., Román-Carraro, F. C., ... & Martínez-Calvillo, S. (2021). Participation of TFIIIB Subunit Brf1 in Transcription Regulation in the Human Pathogen Leishmania major. Genes, 12(2), 280.

Peng, F., Zhou, Y., Wang, J., Guo, B., Wei, Y., Deng, H., ... & Deng, W. (2020). The transcription factor Sp1 modulates RNA polymerase III gene transcription by controlling BRF1 and GTF3C2 expression in human cells. Journal of Biological Chemistry, 295(14), 4617-4630.

Wang, F., Zhao, K., Yu, S., Xu, A., Han, W., & Mei, Y. (2019). RNF12 catalyzes BRF1 ubiquitination and regulates RNA polymerase III–dependent transcription. Journal of Biological Chemistry, 294(1), 130-141.

Khoo, S. K., Wu, C. C., Lin, Y. C., & Chen, H. T. (2018). The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation. Nucleic acids research, 46(3), 1157-1166.

Bellido, F., Sowada, N., Mur, P., Lázaro, C., Pons, T., Valdés-Mas, R., ... & Valle, L. (2018). Association between germline mutations in BRF1, a subunit of the RNA polymerase III transcription complex, and hereditary colorectal cancer. Gastroenterology, 154(1), 181-194.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-BRF1 Recombinant Antibody (CBYJT-1048)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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