Mouse Anti-BRPF1 Recombinant Antibody (2093C5a) (CBMAB-0175-YC)

Name: Mouse Anti-BRPF1 Recombinant Antibody (2093C5a)
SKU: CBMAB-0175-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2093C5a

Application

IP, WB

Immunogen

Recombinant protein corresponding to a region near the C-terminus of Peregrin of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BRPF1

Introduction

BRPF1 (bromodomain and PHD finger containing, 1) is a bromodomain, PHD finger and chromo/Tudor-related Pro-Trp-Trp-Pro (PWWP) domain containing protein. The encoded protein is a component of the MOZ/MORF histone acetyltransferase complexes which function as a transcriptional regulators. BRPF1 is a multivalent chromatin regulator that recognizes different epigenetic marks and may play a role in stimulating acetyltransferase and transcriptional activity of the complex.

Entrez Gene ID

7862

UniProt ID

P55201

Alternative Names

BR140

Function

Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:24065767, PubMed:27939640).
Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PubMed:24065767).
Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation (PubMed:27939640).
Positively regulates the transcription of RUNX1 and RUNX2 (PubMed:18794358).

Biological Process

Histone H3 acetylation Source: UniProtKB
Histone H3-K14 acetylation Source: UniProtKB
Histone H3-K23 acetylation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome

Cellular Location

Nucleus; Cytoplasm; Chromosome. Localization to the nucleus depends on KAT6A, ING5 and MEAF6 (PubMed:18794358, PubMed:27939640). Localizes to transcription start sites (PubMed:24065767).

Involvement in disease

Intellectual developmental disorder with dysmorphic facies and ptosis (IDDDFP): An autosomal dominant neurodevelopmental disorder characterized by delayed psychomotor development, intellectual disability, delayed language, and facial dysmorphisms, most notably ptosis. Additional features may include poor growth, hypotonia, and seizures.

PTM

Acetylated by KAT6A.

Cheng, C. L. H., Tsang, F. H. C., Wei, L., Chen, M., Chin, D. W. C., Shen, J., ... & Wong, C. M. (2021). Bromodomain-containing protein BRPF1 is a therapeutic target for liver cancer. Communications Biology, 4(1), 1-14.

Obi, J. O., Lubula, M. Y., Cornilescu, G., Henrickson, A., McGuire, K., Evans, C. M., ... & Glass, K. C. (2020). The BRPF1 bromodomain is a molecular reader of di-acetyllysine. Current research in structural biology, 2, 104-115.

Klein, B. J., Cox, K. L., Jang, S. M., Côté, J., Poirier, M. G., & Kutateladze, T. G. (2020). Molecular basis for the PZP domain of BRPF1 association with chromatin. Structure, 28(1), 105-110.

Zhu, J., Zhou, C., & Caflisch, A. (2018). Structure-based discovery of selective BRPF1 bromodomain inhibitors. European journal of medicinal chemistry, 155, 337-352.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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