Mouse Anti-C1QTNF4 Recombinant Antibody (CBLC198-LY) (CBMAB-C11305-LY)

The product is antibody recognizes CTRP4. The antibody CBLC198-LY immunoassay techniques such as: WB, IP, IF, ELISA.
See all C1QTNF4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

CBLC198-LY

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, E

Basic Information

Immunogen

Amino acids 35-169 mapping within an internal region of CTRP4 of human origin.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

C1q And TNF Related 4

Entrez Gene ID

Human	114900
Mouse	67445
Rat	311184

UniProt ID

Human	Q9BXJ3
Mouse	Q8R066
Rat	B5DF52

Function

May be involved in the regulation of the inflammatory network. Its role as pro- or anti-inflammatory seems to be context dependent (PubMed:21658842, PubMed:27086950).
Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 h post-injection (By similarity).

Biological Process

Positive regulation of interleukin-6-mediated signaling pathway Source: UniProtKB
Positive regulation of interleukin-6 production Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of tumor necrosis factor production Source: UniProtKB

Cellular Location

Secreted

References

Vester, S. K., Beavil, R. L., Lynham, S., Beavil, A. J., Graham, D. S. C., McDonnell, J. M., & Vyse, T. J. (2021). Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation. Journal of Biological Chemistry, 296.

Vester, S. (2021). Investigating C1QTNF4 in health and systemic lupus erythematosus (Doctoral dissertation, King's College London).

Ye, L., Jia, G., Li, Y., Wang, Y., Chen, H., Yu, L., & Wu, D. (2021). C1q/TNF-related Protein 4 Restores Leptin Sensitivity by Downregulating NF-κB Signaling and Microglial Activation.

Pakzad, B., Shirpour, R., Mousavi, M., Karimzadeh, H., Salehi, A., Kazemi, M., ... & Salehi, R. (2020). C1QTNF4 gene p. His198Gln mutation is correlated with early‐onset systemic lupus erythematosus in Iranian patients. International Journal of Rheumatic Diseases, 23(11), 1594-1598.

Li, Y., Ye, L., Jia, G., Chen, H., Yu, L., & Wu, D. (2020). C1q/TNF-related protein 4 induces signal transducer and activator of transcription 3 pathway and modulates food intake. Neuroscience, 429, 1-9.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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